COBRE: LSU HSC: CELL & MOLECULAR CORE

COBRE：路易斯安那州立大学 HSC：细胞

• 批准号: 7610598
• 负责人: Emel Songu-Mize
• 金额: $ 11.2万
• 依托单位: LSU HEALTH SCIENCES CENTER
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-01 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7610598
• 关键词: Animal Model; Cardiac; Cell Line; Cells; Centers of Research Excellence; Computer Retrieval of Information on Scientific Projects Database; Core Facility; Cultured Cells; Doctor of Philosophy; Embryo; Feeds; Fibroblasts; Fishes; Freezing; Funding; Gel; Grant; Image; Institution; Mass Spectrum Analysis; Mesentery; Mission; Modification; Molecular; Mouse Cell Line; Mus; Neonatal; Numbers; Peptides; Protein Analysis; Proteins; Proteomics; Rattus; Reporting; Research; Research Personnel; Resources; Smooth Muscle Myocytes; Source; Tissue Sample; United States National Institutes of Health; Week; base; instructor; macrophage; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Cell & Molecular Core Emel Songu-Mize, Ph.D. - Director This core has three major support missions: Cell Culture, Proteomics, and Imaging. The facility is staffed by Jesse Guidry, Instructor and Olha Nichols, M.S. CELL CULTURE The core is continuing to supply primary and passaged cells for COBRE investigators. This includes rat aortic smooth muscle cells (users: Drs. Catling, Lucchesi, Wu, Songu-Mize), mouse macrophage cultures (user: Dr. Boulares), rat neonatal cardiac cell cultures (user: Dr. Wu), HUVEC cultures (user: Dr. Boulares). In addition to the above, the Cell Culture core facility developed additional cell lines of mouse mesenteric smooth muscle cells (Drs. Lucchesi, Catling, Songu-Mize), embryonic fibroblasts (Dr. Boulares). The COBRE investigators are provided a weekly report of available cell lines, passage numbers, both the frozen supplies and thriving cultures. They in turn make their request for the following week. Ms. Nichols delivers to each investigator what they need. PROTEOMICS AND IMAGING The Proteomics Core is actively involved in analysis and identification of proteins through several different projects. This core provides a 2DE gel based analysis of protein samples from tissue and cell based experiments. This includes interactome analysis; fishing out proteins that interact with bait proteins (Drs. Varner, Lucchesi, Catling, Lanier and Wu), differential analysis of cell culture treatments (Drs. Boulares, Lanier, Wu) and differential analysis of animal model treatments (Drs. Boulares, Park). The Proteomics Core feeds directly into the LSHHSC Proteomics Core which provides separate and distinct functions through the utilization of mass spectrometry. Through this close association, pure mass spectrometry applications are handled by Dr. Chau-Wen Chou. These include separation and analysis of peptides (Dr. Lazartigues) and modification analysis of proteins and enzymatic targets (Dr. Lanier).

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 细胞和分子核心 Emel Songu-Mize博士- 主任 该核心有三个主要的支持任务：细胞培养，蛋白质组学和成像。该设施的工作人员是杰西Guidry，教练和Olha尼科尔斯，M.S. 细胞培养 核心继续为COBRE研究人员提供原代和传代细胞。 这包括大鼠主动脉平滑肌细胞（用户：Catling、Lucchesi、Wu、Songu-Mize博士）、小鼠巨噬细胞培养物（用户：Boulares博士）、大鼠新生儿心脏细胞培养物（用户：Wu博士）、HUVEC培养物（用户：Boulares博士）。除上述外，细胞培养核心机构还开发了小鼠肠系膜平滑肌细胞（Lucchesi、Catling、Songu-Mize博士）和胚胎成纤维细胞（Boulares博士）的其他细胞系。 每周向COBRE研究者提供一份关于可用细胞系、传代次数、冷冻供应和生长旺盛的培养物的报告。 他们反过来提出下一周的要求。 尼科尔斯女士向每个调查员提供他们需要的东西。 蛋白质组学和成像 蛋白质组学核心通过几个不同的项目积极参与蛋白质的分析和鉴定。该核心提供了基于2DE凝胶的蛋白质样品分析，这些蛋白质样品来自基于组织和细胞的实验。 这包括相互作用组分析; 钓鱼 蛋白质与 诱饵 蛋白质（Varner，Lucchesi，Catling，Lanier和Wu博士），细胞培养处理的差异分析（Boulares，Lanier，Wu博士）和动物模型处理的差异分析（Boulares，Park博士）。 蛋白质组学核心直接输入LSHHSC蛋白质组学核心，后者通过利用质谱法提供单独和独特的功能。 通过这种紧密的联系，纯质谱应用由Chau-Wen Chou博士负责。 这些包括肽的分离和分析（Lazartigues博士）以及蛋白质和酶靶的修饰分析（Lanier博士）。