COBRE: U OF KANSAS MEDICAL CTR: CORE D: PHENOTYPING CORE

COBRE：堪萨斯大学医学 CTR：核心 D：表型核心

• 批准号: 7382249
• 负责人: CURT H. HAGEDORN
• 金额: $ 5.88万
• 依托单位: UNIVERSITY OF KANSAS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-06-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7382249
• 关键词: conditioning; diet; fatty liver; fibrogenesis; gene expression; immunocytochemistry; in situ hybridization; injury; liver; phenotype; proteins; serum; tissues

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Phenotyping Core will support the COBRE projects by facilitating the rapid phenotypic analysis of transgenic or null mice. This Core will do histopathological and immunocytochemistry analysis of livers and serum chemistry of null mice, compared to control mice, as well as under different conditions (diet, drugs, etc). The functions of the Core will include determining gross histological characteristics of tissue, measuring and localizing protein and gene expression in liver or other tissues by immunocytochemistry or in situ hybridization techniques, and performing serum chemistry analysis to follow liver injury and repair during studies of fibrogenesis or fatty liver.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。表型核心将通过促进转基因或无效小鼠的快速表型分析来支持COBRE项目。该核心将对无效小鼠的肝脏和血清化学进行组织病理学和免疫细胞化学分析，与对照小鼠以及不同条件（饮食、药物等）下的分析进行比较。核心的功能包括确定组织的大体组织学特征，通过免疫细胞化学或原位杂交技术测量和定位肝脏或其他组织中的蛋白质和基因表达，以及在纤维化或脂肪肝研究期间进行血清化学分析以跟踪肝损伤和修复。