COBRE: U OF KANSAS MEDICAL CTR: CORE D: PHENOTYPING CORE

COBRE：堪萨斯大学医学 CTR：核心 D：表型核心

• 批准号: 7382249
• 负责人: CURT H. HAGEDORN
• 金额: $ 5.88万
• 依托单位: UNIVERSITY OF KANSAS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-06-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7382249
• 关键词: conditioning; diet; fatty liver; fibrogenesis; gene expression; immunocytochemistry; in situ hybridization; injury; liver; phenotype; proteins; serum; tissues

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Phenotyping Core will support the COBRE projects by facilitating the rapid phenotypic analysis of transgenic or null mice. This Core will do histopathological and immunocytochemistry analysis of livers and serum chemistry of null mice, compared to control mice, as well as under different conditions (diet, drugs, etc). The functions of the Core will include determining gross histological characteristics of tissue, measuring and localizing protein and gene expression in liver or other tissues by immunocytochemistry or in situ hybridization techniques, and performing serum chemistry analysis to follow liver injury and repair during studies of fibrogenesis or fatty liver.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构适用于该中心，这不一定是调查员的机构。表型核心将通过促进转基因或无效小鼠的快速表型分析来支持毛茸茸的项目。与对照小鼠以及不同条件（饮食，药物等）相比，该核心将对肝脏和无效小鼠的血清化学分析进行组织病理学和免疫细胞化学分析。核心的功能将包括确定组织的总体组织学特征，通过免疫细胞化学或原位杂交技术在肝脏或其他组织中测量和定位蛋白质和基因表达，以及进行血清化学分析，以遵循纤维化或脂肪发生期间的肝损伤和修复。