COBRE: U OF KANSAS MEDICAL CTR: CORE B: MOLECULAR BIOLOGY CORE

COBRE：堪萨斯大学医学 CTR：核心 B：分子生物学核心

• 批准号: 7382247
• 负责人: GUOLI DAI
• 金额: $ 7.15万
• 依托单位: UNIVERSITY OF KANSAS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-06-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7382247
• 关键词: bacteria; experience; molecular biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Molecular Biology Core will provide all COBRE scientists with technical support, state-of-the-art equipment, and essential molecular biology reagents, so that experiments which require a molecular biology approach can be carried out in a cost-effective way. The Core will provide a central DNA-sequencing facility, real-time PCR services to quantify mRNA expression, high-throughput PCR and genotyping services, and a central bank of vectors, plasmids, and bacteria for sharing. The Core will provide a central location for essential equipment, including an ABI Prism 3100 Genetic Analyzer (requested in the current application), a real-time PCR machine (in place), a Biomek FX Assay Work Station (liquid dispenser, in place), and 5 thermocyclers (in place), and other commonly used molecular biology equipment, such as an imaging system with the capability for densitometry analysis, bacteria incubators, and ultracentrifuges. The Core w II be staffed with experienced molecular biologists, who not only have hands-on experience in using this equipment, but also a proven track record in managing a core facility.

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。分子生物学核心将为所有Cobre科学家提供技术支持、最先进的设备和必要的分子生物学试剂，以便以经济高效的方式进行需要分子生物学方法的实验。该中心将提供中央DNA测序设施、量化信使核糖核酸表达的实时聚合酶链式反应服务、高通量聚合酶链式反应和基因分型服务，以及用于共享的载体、质粒和细菌的中央银行。核心中心将为基本设备提供一个中心位置，包括一台ABI Prism 3100遗传分析仪(在当前应用中需要)、一台实时聚合酶链式反应机器(就位)、一台Biomek FX分析工作站(就位)和5台热循环仪(就位)，以及其他常用的分子生物学设备，如具有密度分析能力的成像系统、细菌培养箱和超速离心机。Core W II配备了经验丰富的分子生物学家，他们不仅在使用这种设备方面有实践经验，而且在管理核心设施方面也有经过证明的记录。