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Midwest Center for Membrane Protein Structural Dynamics

中西部膜蛋白结构动力学中心

基本信息

批准号：
7498602
负责人：
Eduardo A Perozo
金额：
$ 7.68万
依托单位：
UNIVERSITY OF CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-03-01 至 2010-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7498602
关键词：
ATP phosphohydrolase Acetylcholine Active Sites Address Algorithms Amino Acids Amyloid beta-Protein Precursor Anisotropy Antibodies Applied Genetics Archaea Architecture Area Arts Asses Attention Award Bacteria Bacteriorhodopsins Baculoviruses Behavior Binding Binding Proteins Biochemical Biochemical Reaction Biochemistry Biogenesis Biological Biological Process Biology Biomass Biophysics Catalysis Cell Nucleus Cells Charge Chemicals Chemistry Chicago Childhood Chromosome Pairing Class Classification Code Collaborations Communication Communities Complement Complex Computational algorithm Computing Methodologies Condition Consensus Contracts Copper Core Facility Couples Coupling Crystallization Crystallography Cytokine Receptors DNA DNA Sequence DNA Sequence Rearrangement Data Databases Decompression Sickness Denmark Detergents Deterioration Development Discipline Disease Disease Association Drosophila genus Drug Design Dyes Ecology Ecosystem Electron Spin Resonance Spectroscopy Electron Transport Electrons Electrophysiology (science)Elements End Point Endopeptidases Endpoint Determination Energy Transfer Energy-Generating Resources Engineering Environment Equilibrium Event Evolution Family Figs - dietary Fluorescence Fluorescence Microscopy Fluorescence Resonance Energy Transfer Fluorescence Spectroscopy Fogs Fostering Fourier Transform Free Energy Freedom Funding Gated Ion Channel Gene Amplification Gene Expression Gene Proteins Generations Genetic Genome Genomics Goals Grant Growth Health Heart Heavy Metals Histidine Homeostasis Hormones Hour Human Human Genetics Human Genome Hybrids Hydrolysis Illinois Image Individual Infection Insecta Institutes Institution Integral Membrane Protein Internet Investigation Investments Ion Channel Ions Isotope Labeling Kidney Knockout Mice Knowledge Label Laboratories Lanthanoid Series Elements Lead Leadership Length Libraries Library Services Life Lifting Ligand Binding Ligands Link Liposomes Liquid substance Location Macromolecular Complexes Magnetic Resonance Mammalian Cell Maps Measurable Measurement Measures Mediating Membrane Membrane Biology Membrane Potentials Membrane Proteins Membrane Structure and Function Meta-Analysis Metagenomics Metals Methionine Methodology Methods Microscopic Modeling Modification Molecular Molecular Biology Molecular Chaperones Molecular Conformation Molecular Genetics Molecular Machines Molecular Weight Molecular and Cellular Biology Monitor Motion Movement Multienzyme Complexes Muscle Mutagenesis Myosin ATPase NMR Spectroscopy Na(+)-K(+)-Exchanging ATPase Nature Nerve Neurobiology Neurotransmitter Receptor Noise Nuclear Nuclear Magnetic Resonance Nuclear Receptors Numbers Oligonucleotides Oocytes Operative Surgical Procedures Optical Methods Optics Organism Oxygen PTEN gene Panthera onca Participant Pathway interactions Pediatrics Peptide Hydrolases Peptides Performance Phage Display Pharmacology Phase Phosphatidylinositols Phosphoric Monoester Hydrolases Physical Chemistry Physics Physiologic pulse Physiological Processes Physiology Planets Play Polymerase Chain Reaction Positioning Attribute Post-Translational Protein Processing Potassium Channel Preparation Principal Investigator Process Production Prolactin Receptor Property Protein Analysis Protein Biochemistry Protein Biosynthesis Protein Conformation Protein Dynamics Protein Engineering Protein Region Proteins Proteolysis Proteome Psychological Techniques Publishing Pulse taking Pump Purple Membrane Purpose Range Rate Reaction Reagent Receptor Activation Regulation Relative (related person)Relaxation Reporter Reporting Research Research Infrastructure Research Institute Research Personnel Resolution Resource Allocation Resources Retinal Rhodopsin Roentgen Rays Role Sampling Scaffolding Protein Science Screening procedure Secondary to Secure Series Serine Services Shapes Side Signal Pathway Signal Transduction Simulate Site Site-Directed Mutagenesis Skeletal Muscle Solid Solutions Solvents Somatotropin Source Specific qualifier value Spectroscopy, Fourier Transform Infrared Spectrum Analysis Speed Spin Labels Staging Standards of Weights and Measures Statistical Mechanics Stimulus Stream Structural Protein Structure Support of Research Surface Synapses Synaptic Transmission System Systems Biology Techniques Technology Terbium Tertiary Protein Structure Testing Time Travel United States National Institutes of Health Universities Vertebral column Water Weight Work X-Ray Crystallography Yeasts amyloid precursor protein processing anticancer research aqueous base cell growth cell motility chemical addition chemical bond chemical synthesis combinatorial computer studies computerized tools concept conformational conversion covalent bond cytokine day density design design and construction electric field electrical measurement electron donor expectation experience feeding improved in vivo innovation insight interest ligand gated channel luminescence resonance energy transfer macromolecule magnetic field member metagenomic sequencing microbial community microorganism milligram millisecond molecular dynamics molecular recognition multidisciplinary mutant nanodevice nanomachine nanosecond nitroxyl novel novel strategies parallel computing particle patch clamp peptide chemical synthesis phosphorescence presenilin-1 programs protein expression protein folding protein function protein protein interaction protein purification protein structure quantum quantum chemistry receptor receptor function reconstitution research study response rhomboid scaffold secretase sensor simulation single molecule steroid hormone structural biology structural genomics success tetramethylrhodamine theories three dimensional structure tool trafficking voltage voltage gated channel water channel yeast two hybrid system

项目摘要

DESCRIPTION (provided by applicant): Membrane proteins play an essential role in controlling the movement of material and information in and out of the cell, in determining the flow and use of energy, as well as in triggering the initiation of numerous signaling pathways. To fulfill these roles, conformational and interaction dynamics exert a dominant influence on their functional behavior, for it is the interplay between structure and dynamics what ultimately defines their function. The Midwest Center for Membrane Protein Structural Dynamics (MMPSD) is proposed as a highly interactive, tightly integrated and multidisciplinary effort focused on elucidating the relationship between structure, free energy landscapes, dynamics and function. The MMPSD will be organized around multidisciplinary project teams with investigators from institutions clustered geographically inn [sic] the Midwest to maximize true interactive collaborations and an efficient exchange of ideas. These teams will study major mechanistic questions associated with membrane protein function as it relates to three major areas: energy transduction in signaling (ion channels and receptors) energy interconversion (transporters and pumps) and chemo-transduction pathways (membrane-embedded proteases and phosphatases). Our ultimate goals is to decode the general mechanistic principles that govern protein movement and its associated fluctuation dynamics by dissecting and analyzing the molecular and dynamical bases of these functions at an unprecedented and quantitative level, as well as exploiting this information to engineer altered and novel activities into membrane protein frameworks to rationally evolve new functions. To accomplish its goals, the MMPSD will develop in parallel a set of tools, concepts and reagents to: 1) Determine time-averaged structures of "Archetype" membrane proteins using Chaperone-assisted crystallization methods; 2) Apply state of the art spectroscopic methods (Magnetic Resonance, Fluorescence) to follow conformational changes and dynamics of the determined structures; and 3) Design and implement novel computational approaches to link static and dynamic data with function. Four core facilities will feed and interconnect with the individual projects in a highly interactive way. The cores will support the research in the Center by providing service and expertise in four critical areas: Membrane protein expression, the establishment of chemical synthesis capabilities for probes and detergents, the generation of a large variety of crystallization chaperones and other target binders, and generation of a pipeline of novel membrane targets through metagenomics approaches. All of the information, tools and new reagents/targets will be shared with the research community at large through the "membrane protein dynamics gateway", a state of the art web page and a series of scientific meetings open to the public.

描述（由申请人提供）：膜蛋白在控制物质和信息进出细胞的运动，决定能量的流动和使用以及触发许多信号通路的启动方面起着至关重要的作用。为了完成这些角色，构象动力学和相互作用动力学对它们的功能行为施加了主要影响，因为结构和动力学之间的相互作用最终决定了它们的功能。中西部膜蛋白结构动力学中心（MMPSD）是一个高度互动，紧密集成和多学科的努力，专注于阐明结构，自由能景观，动力学和功能之间的关系。