Matrix Proteins of the plant ER-Golgi interface

植物内质网-高尔基体界面的基质蛋白

• 批准号: BB/F008147/1
• 负责人: Christopher Hawes
• 金额: $ 36.45万
• 依托单位: Oxford Brookes University
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2008
• 资助国家: 英国
• 起止时间: 2008 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FF008147%2F1
• 关键词: Matrix; Proteins; plant; ER; Golgi

The Golgi apparatus is a key component within cells which is involved in the processing and manufacture of proteins, carbohydrates and membranes which are targeted to other compartments of the cells of secreted to the external environment. We have previously shown that in plant cells the Golgi apparatus is unique consisting of numerous stacks of membrane-bounded discs called cisternae distributed within the cell. In many cell types these Golgi stacks are also very closely associated with another membrane network, the endoplasmic reticulum that passes biosynthetic cargo to the Golgi for processing. One remarkable feature of the plant Golgi, which distinguishes it from that in animal and fungal cells, is that the stacks are highly motile and in many tissues including leaves, move over the surface of the endoplasmic reticulum. The key questions that we are going to address in this research programme are how does the Golgi stay attached to the ER and how do the individual stacks of cisternae remain intact when they are moving through the cytoplasm? Over recent years a number of proteins have been discovered that may potentially act as anchors or tethers that help hold the Golgi together and which may also act as linkers for transport vesicles produced by the endoplasmic reticulum. We have recently identified a number of proteins that are good candidates for the plant tethering proteins including one novel protein. Using the technology of fusing these to fluorescent marker proteins we have shown these to locate to the part of the Golgi nearest to the endoplasmic reticulum. The work proposed in this grant will involve investigating the interaction of these proteins with each other and the regulation of these proteins. Their role in organising the Golgi stack will be assessed by expressing in leaves mutant forms of the proteins and looking for both morphological and biochemical effects on the Golgi apparatus and the secretion of proteins. We will also investigate the role these proteins may have in the biogenesis of new Golgi stacks from the endoplasmic reticulum.

高尔基体是细胞内的关键组成部分，参与蛋白质、碳水化合物和膜的加工和制造，这些蛋白质、碳水化合物和膜的目标是分泌到外部环境的细胞的其他区室。我们之前已经证明，在植物细胞中，高尔基体是独特的，由分布在细胞内的许多堆叠的膜边界盘（称为池）组成。在许多细胞类型中，这些高尔基体堆叠也与另一个膜网络（将生物合成物质传递到高尔基体进行处理的内质网）密切相关。植物高尔基体与动物和真菌细胞的区别在于，植物高尔基体的一个显着特征是高尔基体高度活动，并且在包括叶子在内的许多组织中，在内质网表面移动。我们在这个研究项目中要解决的关键问题是高尔基体如何保持附着在内质网上，以及当它们穿过细胞质时，各个池如何保持完整？近年来，人们发现了许多蛋白质，它们可能充当锚或系绳，帮助将高尔基体固定在一起，也可能充当内质网产生的运输囊泡的连接物。我们最近鉴定了许多蛋白质，它们是植物束缚蛋白的良好候选者，其中包括一种新蛋白质。利用将它们与荧光标记蛋白融合的技术，我们已经证明它们能够定位到高尔基体最靠近内质网的部分。这笔资助中提出的工作将涉及研究这些蛋白质之间的相互作用以及这些蛋白质的调节。它们在组织高尔基体堆栈中的作用将通过在叶子中表达突变形式的蛋白质并寻找对高尔基体和蛋白质分泌的形态和生化影响来评估。我们还将研究这些蛋白质在内质网新高尔基体堆叠的生物发生中可能发挥的作用。

项目成果

Golgi membrane dynamics after induction of a dominant-negative mutant Sar1 GTPase in tobacco.

• DOI: 10.1093/jxb/erp315
• 发表时间: 2010
• 期刊: Journal of experimental botany
• 影响因子: 6.9
• 作者: A. Osterrieder;E. Hummel;Claudine M Carvalho;C. Hawes

Endomembrane and Golgi traffic in plant cells.

• DOI: 10.1016/b978-0-12-417164-0.00005-7
• 发表时间: 2013
• 期刊: METHODS IN CELL BIOLOGY
• 作者: Stefano, Giovanni;Osterrieder, Anne;Hawes, Chris;Brandizzi, Federica
• 通讯作者: Brandizzi, Federica

Stacks off tracks: a role for the golgin AtCASP in plant endoplasmic reticulum-Golgi apparatus tethering.

• DOI: 10.1093/jxb/erx167
• 发表时间: 2017-06-15
• 期刊: Journal of experimental botany
• 影响因子: 6.9
• 作者: Osterrieder A;Sparkes IA;Botchway SW;Ward A;Ketelaar T;de Ruijter N;Hawes C
• 通讯作者: Hawes C

Sequential depletion and acquisition of proteins during Golgi stack disassembly and reformation.

高尔基体堆叠拆卸和重组过程中蛋白质的连续消耗和获取。

• DOI: 10.1111/j.1600-0854.2010.01106.x
• 发表时间: 2010
• 期刊: Traffic (Copenhagen, Denmark)
• 作者: Schoberer J