Sequencing the 'gene space' of potato chromosome IV comparative analysis with tomato and development of a gene-based mapping platform

马铃薯 IV 号染色体“基因空间”测序与番茄比较分析及基于基因的作图平台开发

• 批准号: BB/F013280/1
• 负责人: Gerard Bishop
• 金额: $ 3.74万
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2008
• 资助国家: 英国
• 起止时间: 2008 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FF013280%2F1
• 关键词: Sequencing; gene; space; potato; chromosome

This proposal will allow the UK to participate in an international collaboration to determine the DNA sequence of the 'gene-rich' regions of the potato genome, by sequencing chromosome IV. The other chromosomes are being sequenced by groups in other countries. Potato, the world's fourth most important food crop, is the most important member of the Solanaceae, which also includes tomato, pepper, aubergine and many medically relevant plants. The potato genome is very similar to tomato and, as the UK is also sequencing tomato chromosome IV, we will be able to perform a highly detailed comparative sequence analysis of these two crop species. Moreover, by studying gene and genome sequence data we will be able to create a 'genetic marker' system, based on small DNA variations between different varieties. This will allow us to readily examine a large number of genes and to see how they vary between varieties with different characteristics. This will be extremely useful for efforts to breed potato crops that can be grown in a more environmentally friendly and sustainable manner. All of the data generated will be placed in the public domain in a user-ready format with the identification of putative genes and their function. The project will also be used to bring the UK research community working on Solanaceous plants together each year for the purposes of communicating the results of their research.

这项提议将使英国能够参与一项国际合作，通过对第四号染色体进行测序，确定马铃薯基因组“基因丰富”区域的DNA序列。其他国家的小组正在对其他染色体进行测序。马铃薯是世界上第四大粮食作物，是茄科最重要的成员，茄科还包括番茄、胡椒、茄子和许多医学相关植物。马铃薯基因组与番茄非常相似，由于英国也在对番茄IV号染色体进行测序，我们将能够对这两种作物物种进行非常详细的比较序列分析。此外，通过研究基因和基因组序列数据，我们将能够创建一个“遗传标记”系统，基于不同品种之间的小DNA变异。这将使我们能够容易地检查大量的基因，并了解它们在具有不同特征的品种之间的变化。这对于培育能够以更环保和可持续的方式种植的马铃薯作物的努力将是非常有用的。所有生成的数据将以用户准备好的格式放置在公共领域，并识别推定的基因及其功能。该项目还将用于每年将英国研究茄科植物的研究团体聚集在一起，以交流他们的研究成果。

项目成果

Construction of reference chromosome-scale pseudomolecules for potato: integrating the potato genome with genetic and physical maps.

• DOI: 10.1534/g3.113.007153
• 发表时间: 2013-11-06
• 期刊: G3 (Bethesda, Md.)
• 作者: Sharma SK;Bolser D;de Boer J;Sønderkær M;Amoros W;Carboni MF;D'Ambrosio JM;de la Cruz G;Di Genova A;Douches DS;Eguiluz M;Guo X;Guzman F;Hackett CA;Hamilton JP;Li G;Li Y;Lozano R;Maass A;Marshall D;Martinez D;McLean K;Mejía N;Milne L;Munive S;Nagy I;Ponce O;Ramirez M;Simon R;Thomson SJ;Torres Y;Waugh R;Zhang Z;Huang S;Visser RG;Bachem CW;Sagredo B;Feingold SE;Orjeda G;Veilleux RE;Bonierbale M;Jacobs JM;Milbourne D;Martin DM;Bryan GJ
• 通讯作者: Bryan GJ