CDC14 PHOSPHORYLATION

CDC14磷酸化

• 批准号: 7355106
• 负责人: RAYMOND J DESHAIES
• 金额: $ 0.12万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7355106
• 关键词: CDC14; PHOSPHORYLATION

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We use our newly developed method for the isolation of GFP-tagged proteins to study phosphorylation of the phosphatase, Cdc14,required for cell cycle progression. GFP-tagged Cdc14, present at endogenous levels, was isolated from 16 L of yeast culture using magnetic beads coated with GFP Ab. Following digestion with trypsin (using various digestion conditions), several predicted sites of phosphorylation were analyzed by MS, MS/MS analyses and hypothesis driven mass spectrometry analysis.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是中心的机构，不一定是研究者的机构。我们使用新开发的分离 GFP 标记蛋白的方法来研究细胞周期进展所需的磷酸酶 Cdc14 的磷酸化。使用 GFP Ab 包被的磁珠从 16 L 酵母培养物中分离出内源水平存在的 GFP 标记的 Cdc14。用胰蛋白酶消化（使用不同的消化条件）后，通过 MS、MS/MS 分析和假设驱动的质谱分析来分析几个预测的磷酸化位点。