INTERACTION OF RNA POLYMERASE WITH TRANS-ACTING FACTORS

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。在大肠杆菌中，转录是由一个单一的酶：RNA聚合酶(RNAP)驱动的。为了识别启动子、转录基因和终止转录，RNAP必须与反式作用因子相互作用。我们与Chait实验室合作的工作的一个方面是在细胞周期的不同阶段识别与大肠杆菌RNAP相关的因素，并对不同的刺激做出反应。以前未知的RNAP结合蛋白将使用遗传、生化和结构技术进行更详细的研究，以确定它们在转录周期中的作用。噬菌体为生物学的发育研究提供了一个极好而美丽的模型。在感染噬菌体后，E.ColiRNAP被噬菌体编码的蛋白定位，这些蛋白与宿主RNAP结合并使其适合于转录噬菌体编码的基因。因此，我们工作的第二个方面，也是与Chait实验室合作，将涉及从一些与E.ColiRNAP相关的不同噬菌体中鉴定蛋白质。这些噬菌体编码的RNAP结合蛋白的鉴定将是了解噬菌体发育的第一步，并可能作为一类抗菌剂。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In Escherichia coli (E. coli), transcription is driven by a single enzyme: RNA Polymerase (RNAP). In order to recognize promoters, transcribe genes, and terminate transcription, RNAP must interact with trans-acting factors. One aspect of our work, in collaboration with the Chait laboratory, is to identify those factors that associate with E. coli RNAP at different stages of the cell cycle, and in response to different stimuli. Previously unidentified RNAP-binding proteins will be studied in greater detail using genetic, biochemical, and structural techniques to determine their contribution to the transcription cycle. Bacteriophage offer an excellent and beautiful model for the study of development in biology. Upon infection with phage, E. coli RNAP is targeted by phage encoded proteins that bind to and appropriate the host RNAP to transcribe phage encoded genes. Thus, the second aspect of our work, again in collaboration with the Chait laboratory, will involve the identification of proteins from a number of different bacteriophage that associate with E. coli RNAP. Identification of these phage encoded RNAP-binding proteins will be the first step in understanding the development of the bacteriophage, and may serve as a class of antimicrobial.