DETERMINE THE STRUCTURAL BASIS FOR ?N INTERACTIONS WITH ITS PROMOTER DNA

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 <$N（或<$54）家族的<$因子与<$70家族（大多数<$因子属于该家族）在进化上没有关系。N在序列和功能上是不同的。虽然…70家族的结构/功能关系已经建立在坚实的基础上，关于N的结构信息仅限于与启动子片段结合的7 kDa C-末端“RpoN”结构域的NMR结构（ 24元件）和参与核心RNAP结合的N-末端结构域。更完整的结构表征是很有意义的。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The ¿N (or ¿54) family of ¿ factors is not evolutionarily related to the ¿70 family (to which most ¿ factors belong). The ¿N is distinct in sequence and function. While the ¿70 family structure/function relationship has been placed on a firm basis, structural information on ¿N is limited to NMR structures of the 7 kDa C-terminal 'RpoN' domain bound to a promoter fragment (the 24 element) and an N-terminal domain involved in core RNAP binding. More complete structural characterization of ¿N is of great interest.