?/ANTI-? COMPLEXES: STAPHYLOCOCCAL AUREUS PHAGE G1 ORF67
?/ANTI-? COMPLEXES: STAPHYLOCOCCAL AUREUS PHAGE G1 ORF67
批准号:
8169306
负责人:
Seth A. Darst
金额:
$0.2万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2010
资助国家:
美国
项目状态:
已结题
起止时间:
2010-04-01 至 2011-03-31
关键词:
BacteriaBacteriophagesBindingBiologyC-terminalCell physiologyComplexComputer Retrieval of Information on Scientific Projects DatabaseCuesEnvironmentFundingGenetic TranscriptionGoalsGrantGrowthInstitutionProteinsRegulationRegulonResearchResearch PersonnelResourcesResponse ElementsRoleSignal TransductionSourceStaphylococcus aureusStructureUnited States National Institutes of Healthhigh throughput screeninginhibitor/antagonistpathogenresponse
中文摘要
这个子项目是众多研究子项目之一
英文摘要
This subproject is one of many research subprojects utilizing the
resources provided by a Center grant funded by NIH/NCRR. The subproject and
investigator (PI) may have received primary funding from another NIH source,
and thus could be represented in other CRISP entries. The institution listed is
for the Center, which is not necessarily the institution for the investigator.
Bacterial transcription depends on a primary (group 1) ¿ that is essential for viability. In addition, most bacteria contain alternative ¿'s that control regulons in response to environmental cues. Regulation of ¿ activity is a major mechanism by which bacteria respond to their environment. Many alternative ¿'s are regulated by anti-¿ factors. Despite the structural conservation of ¿'s, anti-¿'s are structurally and functionally diverse, serving as the key response elements to sense and signal environmental cues to the core transcriptional apparatus via the alternative ¿'s.
In addition to modulation of ¿ function by cellular anti-¿ factors, many bacteriophages have evolved ingenious mechanisms to inhibit and/or appropriate the host transcription apparatus. The primary ¿ (¿A) of the gram-positive pathogen Staphylococcus aureus (Sau) is targeted by phage proteins that inhibit bacterial growth. Phage G1 ORF67 was identified in a high-throughput screen as a transcriptional inhibitor that binds to the C-terminal domain (domain 4) of Sau ¿A (¿A4). Our goal is to determine the co-crystal structure of G1 ORF67 with Sau ¿A4, and to explore the functional mechanism through which ORF67 inhibits Sau transcription as well as it's role in phage G1 biology.
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Structure, function, and regulation of the bacterial transcription cycle
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批准号:10607993
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项目类别:
-
资助金额:$83.56万
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财政年份:2016
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负责人:Seth A. Darst
-
依托单位:
Structure, function, and regulation of the bacterial transcription cycle
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批准号:10394344
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项目类别:
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资助金额:$83.56万
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财政年份:2016
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负责人:Seth A. Darst
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依托单位:
Structure, function, and regulation of the bacterial transcription cycle
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批准号:10388954
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项目类别:
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资助金额:$5.36万
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财政年份:2016
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负责人:Seth A. Darst
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依托单位:
Structure, function, and regulation of the bacterial transcription cycle
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批准号:9921406
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项目类别:
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资助金额:$81.12万
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财政年份:2016
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负责人:Seth A. Darst
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依托单位:
Structure, function, and regulation of the bacterial transcription cycle
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批准号:9071516
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项目类别:
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资助金额:$79.1万
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财政年份:2016
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负责人:Seth A. Darst
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依托单位:
Structure, function, and regulation of the bacterial transcription cycle
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批准号:9271202
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项目类别:
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资助金额:$81.12万
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财政年份:2016
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负责人:Seth A. Darst
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依托单位:
Structural studies of RNA polymerase regulation by RNA
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批准号:8238020
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项目类别:
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资助金额:$34.02万
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财政年份:2012
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负责人:Seth A. Darst
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依托单位:
Structural studies of RNA polymerase regulation by RNA
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批准号:8794441
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项目类别:
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资助金额:$32.98万
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财政年份:2012
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负责人:Seth A. Darst
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依托单位:
Structural studies of RNA polymerase regulation by RNA
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批准号:8431355
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项目类别:
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资助金额:$31.83万
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财政年份:2012
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负责人:Seth A. Darst
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依托单位:
Structural studies of RNA polymerase regulation by RNA
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批准号:8608542
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项目类别:
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资助金额:$32.98万
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财政年份:2012
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负责人:Seth A. Darst
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依托单位:
STRUCTURAL STUDIES OF BACTERIAL SIGNALLING: SPORULATION CONTROL
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批准号:8169240
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项目类别:
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资助金额:$0.2万
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财政年份:2010
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负责人:Seth A. Darst
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依托单位:
A GENERIC METHOD TO STUDY BACTERIOPHAGE/HOST INTERACTIONS
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批准号:8169128
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项目类别:
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资助金额:$0.12万
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财政年份:2010
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负责人:Seth A. Darst
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依托单位:
STRUCTURAL BASIS FOR MICROTUBULE CROSSLINKING BY THE MAP65 PROTEIN FAMILY
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批准号:8169310
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项目类别:
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资助金额:$0.2万
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财政年份:2010
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负责人:Seth A. Darst
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依托单位:
Bacterial RNAP sigma factor structure and function
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批准号:8055634
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项目类别:
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资助金额:$2.26万
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财政年份:2010
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负责人:Seth A. Darst
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依托单位:
CRYSTAL STRUCTURES OF THE GLYCOPEPTIDE SULFOTRANSFERASE TEG12
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批准号:8169309
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项目类别:
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资助金额:$0.2万
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财政年份:2010
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负责人:Seth A. Darst
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依托单位:
STRUCTURE OF A PAUSED TRANSCRIPTION ELONGATION COMPLEX
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批准号:8169308
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项目类别:
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资助金额:$0.2万
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财政年份:2010
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负责人:Seth A. Darst
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依托单位:
BACTERIAL RNA POLYMERASE ? FACTOR INTERACTIONS WITH THE PROMOTER -10 ELEMENT
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批准号:8169305
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项目类别:
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资助金额:$0.2万
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财政年份:2010
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负责人:Seth A. Darst
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依托单位:
DETERMINE THE STRUCTURAL BASIS FOR ?N INTERACTIONS WITH ITS PROMOTER DNA
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批准号:8169307
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项目类别:
-
资助金额:$0.2万
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财政年份:2010
-
负责人:Seth A. Darst
-
依托单位:
A GENERIC METHOD TO STUDY BACTERIOPHAGE/HOST INTERACTIONS
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批准号:7954085
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项目类别:
-
资助金额:$0.59万
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财政年份:2009
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负责人:Seth A. Darst
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依托单位:
STRUCTURAL STUDIES OF BACTERIAL SIGNALLING: SPORULATION CONTROL
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批准号:7955130
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项目类别:
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资助金额:$2.5万
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财政年份:2009
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负责人:Seth A. Darst
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依托单位:
海外基金