A tissue engineered corneal epithelium replacement for animal testing using human stem cells

使用人类干细胞进行动物试验的组织工程角膜上皮替代物

基本信息

  • 批准号:
    BB/F019742/1
  • 负责人:
  • 金额:
    $ 42.15万
  • 依托单位:
  • 依托单位国家:
    英国
  • 项目类别:
    Research Grant
  • 财政年份:
    2008
  • 资助国家:
    英国
  • 起止时间:
    2008 至 无数据
  • 项目状态:
    已结题

项目摘要

The use of human stem cells in the treatment of serious disease or injury is currently undergoing a phase of rapid development. This intensive research has already produced new treatments for blindness. UK and Japanese scientists are now growing adult stem cells taken from donor corneas (the transparent tissue at the front of the eye) on specialised membranes to create new functional tissue. These regenerated tissues are then transplanted onto the surface of diseased or injured eyes leading to a restoration of the patients' sight. The use of human stem cells need not be limited to clinical theraputic use i.e. aimed at re-establishing vision in humans, they can aslo be used to significantly reduce the number of animals used in medical research. Presently, thousands of rabbits are needed every year to test for the toxic potential of new drugs and cosmetics. The method used in testing on rabbits eyes has changed little over 50 years. Now, by employing the scientific advancements recently made in therapeutic stem cell research to the manufacture of a human tissue based replica in culture, we aim to develop a human stem cell based non-animal alternative to drug and nanomaterial testing. The advantages of this are primarily that the effect of new chemicals/materials can be assessed without any animals being harmed and since the replica tissue is formed from human stem cells the results are also more representative. Furthermore, our non-animal model can assess water insoluble materials such as nanomaterials, something the animal models can not do well. To develop a human stem cell based non-animal alternative to drug testing we will essentially recreate the surface of the human eye in a plastic dish. Using adult stem cells isolated from adult corneal donor tissue and building upon the techniques sucessfully employed for corneal stem cell transplantation, we will identify a suitable protocol and substrate capable of both supporting the formation of a functional corneal epithelium in vitro and being easily sourced. The suitability of the newly developed human stem cell based non-animal alternative to drug testing will be established by vigorous testing of the model's response to known toxic and non-toxic agents. The model's response to toxicity testing will be robustly assessed by microscopy and established toxicity assays.
利用人类干细胞治疗严重疾病或损伤目前正处于快速发展阶段。这项密集的研究已经产生了治疗失明的新方法。英国和日本的科学家现在正在培养从捐赠者的角膜(眼睛前面的透明组织)中提取的成体干细胞,在特制的膜上培养,以创造新的功能组织。这些再生的组织然后被移植到患病或受伤的眼睛表面,从而恢复患者的视力。人类干细胞的使用不必局限于临床治疗用途,即旨在重建人类视力的用途,它们也可以用于显著减少医学研究中使用的动物数量。目前,每年需要数千只兔子来测试新药和化妆品的毒性潜力。在兔眼上进行测试的方法在过去50年里几乎没有变化。现在,通过利用最近在治疗性干细胞研究中取得的科学进步来制造基于人类组织的培养复制品,我们的目标是开发一种基于人类干细胞的非动物替代药物和纳米材料测试。这样做的主要好处是,可以在不伤害任何动物的情况下评估新化学品/材料的效果,而且由于复制组织是由人类干细胞形成的,结果也更具代表性。此外,我们的非动物模型可以评估不溶于水的材料,如纳米材料,这是动物模型做不到的。为了开发一种基于人类干细胞的非动物药物测试替代方案,我们将基本上在塑料盘子中重建人类眼睛的表面。利用从成人角膜供体组织中分离的成人干细胞,并在成功应用于角膜干细胞移植的技术基础上,我们将确定一种合适的方案和底物,既能支持在体外形成有功能的角膜上皮,又能容易获得来源。新开发的基于人类干细胞的非动物替代药物测试的适用性将通过对该模型对已知有毒和无毒药物的反应进行严格测试来确定。该模型对毒性测试的反应将通过显微镜和已建立的毒性测试进行强有力的评估。

项目成果

期刊论文数量(10)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Investigation of K14/K5 as a stem cell marker in the limbal region of the bovine cornea.
  • DOI:
    10.1371/journal.pone.0013192
  • 发表时间:
    2010-10-06
  • 期刊:
  • 影响因子:
    3.7
  • 作者:
    Chen B;Mi S;Wright B;Connon CJ
  • 通讯作者:
    Connon CJ
The formation of a tissue-engineered cornea using plastically compressed collagen scaffolds and limbal stem cells.
  • DOI:
    10.1007/978-1-62703-432-6_9
  • 发表时间:
    2013
  • 期刊:
  • 影响因子:
    0
  • 作者:
    S. Mi;C. Connon
  • 通讯作者:
    S. Mi;C. Connon
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Che Connon其他文献

Che Connon的其他文献

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{{ truncateString('Che Connon', 18)}}的其他基金

Development of Brillouin Spectroscopy for Mechanotransduction Research
用于力传导研究的布里渊光谱学的发展
  • 批准号:
    BB/N021576/1
  • 财政年份:
    2016
  • 资助金额:
    $ 42.15万
  • 项目类别:
    Research Grant
Bioprosthetic cornea: using polymeric templates for directed stem cell growth
生物假体角膜:使用聚合物模板进行干细胞定向生长
  • 批准号:
    BB/I008187/2
  • 财政年份:
    2014
  • 资助金额:
    $ 42.15万
  • 项目类别:
    Research Grant
Modulation of limbal niche stiffness to regulate stem cell differentiation
调节角膜缘生态位硬度以调节干细胞分化
  • 批准号:
    MR/K017217/2
  • 财政年份:
    2014
  • 资助金额:
    $ 42.15万
  • 项目类别:
    Research Grant
Investigation of optimal gel conditions for stem cell preservation at room temperature and scaling up of selected methodology
研究室温下保存干细胞的最佳凝胶条件并扩大所选方法的规模
  • 批准号:
    BB/K011111/2
  • 财政年份:
    2014
  • 资助金额:
    $ 42.15万
  • 项目类别:
    Research Grant
Modulation of limbal niche stiffness to regulate stem cell differentiation
调节角膜缘生态位硬度以调节干细胞分化
  • 批准号:
    MR/K017217/1
  • 财政年份:
    2013
  • 资助金额:
    $ 42.15万
  • 项目类别:
    Research Grant
Investigation of optimal gel conditions for stem cell preservation at room temperature and scaling up of selected methodology
研究室温下保存干细胞的最佳凝胶条件并扩大所选方法的规模
  • 批准号:
    BB/K011111/1
  • 财政年份:
    2013
  • 资助金额:
    $ 42.15万
  • 项目类别:
    Research Grant
Bioprosthetic cornea: using polymeric templates for directed stem cell growth
生物假体角膜:使用聚合物模板进行干细胞定向生长
  • 批准号:
    BB/I008187/1
  • 财政年份:
    2011
  • 资助金额:
    $ 42.15万
  • 项目类别:
    Research Grant
Cold chain storage and distribution of therapeutic mammalian cell cultures including stem cells using sol-gel technology
使用溶胶凝胶技术冷链储存和分配包括干细胞在内的治疗性哺乳动物细胞培养物
  • 批准号:
    BB/I00985X/1
  • 财政年份:
    2011
  • 资助金额:
    $ 42.15万
  • 项目类别:
    Research Grant
Therapeutic corneal stem cell delivery using hydrogels without the need for ex vivo expansion
使用水凝胶进行治疗性角膜干细胞递送,无需离体扩增
  • 批准号:
    G0900877/1
  • 财政年份:
    2010
  • 资助金额:
    $ 42.15万
  • 项目类别:
    Research Grant

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重复荷载作用下ECC材料的疲劳性能及力学模型研究
  • 批准号:
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A human tissue-engineered three-layer cornea (hTEC) supplemented with macrophages as a biomaterial for in vitro studies
补充有巨噬细胞的人体组织工程三层角膜(hTEC)作为体外研究的生物材料
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  • 批准号:
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Urothelial Stem Cells
尿路上皮干细胞
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    9383628
  • 财政年份:
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Controlling Cell Phenotype in a Tissue-Engineered Corneal Model
控制组织工程角膜模型中的细胞表型
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    8812691
  • 财政年份:
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    $ 42.15万
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MODIFICATION OF CORNEAL TISSUE ENGINEERED SCAFFOLDS TO PROMOTE EPITHELIALIZATION
改良角膜组织工程支架以促进上皮化
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  • 财政年份:
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    $ 42.15万
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MODIFICATION OF CORNEAL TISSUE ENGINEERED SCAFFOLDS TO PROMOTE EPITHELIALIZATION
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