MODIFICATION OF CORNEAL TISSUE ENGINEERED SCAFFOLDS TO PROMOTE EPITHELIALIZATION

改良角膜组织工程支架以促进上皮化

• 批准号: 7609952
• 负责人: Ping-Fai Sit
• 金额: $ 5.36万
• 依托单位: LOUISIANA STATE UNIV A&M COL BATON ROUGE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7609952
• 关键词: Anterior; Biological; Biomimetics; Cellular biology; Chemistry; Computer Retrieval of Information on Scientific Projects Database; Cornea; Epithelial; Epithelial Cells; Extracellular Matrix Proteins; Extracellular Protein; Facility Construction Funding Category; Funding; Gelatin; Grant; Hydrogels; Institution; Laminin; Mechanics; Methods; Modification; Nature; Optics; Peptides; Permeability; Property; Proteins; Research; Research Personnel; Resources; Science; Source; Stromal Cells; Surface; Techniques; Tissue Engineering; United States National Institutes of Health; Wettability; biomaterial compatibility; cell growth; corneal epithelium; hydrophilicity; nanoengineering; nanofabrication; novel; research study; response; scaffold; size; solute

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our approach in developing biomimetic corneal material is to combine multiple material science strategies for the construction of a tissue engineered scaffold, which requires a minimum 2-layer microstructure that specifically separates the anterior epithelial layer from the stromal cell layer. Additionally, novel nanoengineering techniques will allow us to screen multiple extracellular proteins (ECMPs) and peptide ratios and concentrations to determine the optical surface for enhanced cellular response. Then, covalent tethering of ECMPs and peptides to the scaffold surfaces will increase the biomimetic nature of the surfaces and enhance specific cell growth. In this project, we are integrating novel chemistry, micro/nanofabrication, and cell biology methods to first understand and then control both corneal epithelial cell-material interactions. In the previous project period, a novel hydrogel scaffold material, PAH/GMA, was developed. In this study period, we plan to completely characterize this PAH/GMA material in terms of: 1) pore size and solute perturbation/permeability; 2) surface hydrophilicity/wettability; 3) physical integrity and mechanical properties; 4) optical clarity; and 5) biological stability. Additionally, preliminary cellular compatibility studies were performed with a non-biologically relevant protein, gelatin. We will repeat the cellular compatibility experiments using laminin, a corneal extracellular matrix protein, to confirm the epithelial cell biocompatibility of our novel material.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们开发仿生角膜材料的方法是将联合收割机多种材料科学策略结合起来，用于构建组织工程支架，这需要至少2层微结构，专门将前上皮层与基质细胞层分开。此外，新的纳米工程技术将使我们能够筛选多种细胞外蛋白（ECMPs）和肽的比例和浓度，以确定增强细胞反应的光学表面。然后，将ECMP和肽共价拴系到支架表面将增加表面的仿生性质并增强特异性细胞生长。在这个项目中，我们正在整合新的化学，微/纳米纤维和细胞生物学方法，首先了解然后控制角膜上皮细胞-材料相互作用。在上一个项目期间，开发了一种新型水凝胶支架材料PAH/GMA。在本研究期间，我们计划在以下方面对该PAH/GMA材料进行全面表征：1）孔径和溶质扰动/渗透性; 2）表面亲水性/润湿性; 3）物理完整性和机械性能; 4）光学透明度; 5）生物稳定性。此外，使用非生物学相关蛋白质明胶进行了初步细胞相容性研究。我们将使用层粘连蛋白（一种角膜细胞外基质蛋白）重复细胞相容性实验，以确认我们的新型材料的上皮细胞生物相容性。