CORE--LIGAND ASSAY AND ANALYSIS FACILITY

核心——配体测定和分析设施

• 批准号: 6744676
• 负责人: DANIEL J. HAISENLEDER
• 金额: $ 12.24万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-23 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6744676
• 关键词: biomedical facility; cooperative study; immunologic assay /test; immunologic substance development /preparation; method development; polycystic ovary syndrome; training

The Ligand Assay and Analysis Core will serve an essential role in this U54 Center. First, the Core will measure peptide and steroid reproductive hormones in blood and tissue samples by immunoassay. Second, develop new state-of-the-art assay methods to accommodate the needs of the U54 investigators. Third, prepare labeled ligands (peptides, antibodies) for use in immunoassays, immunocytochemistry or binding studies. Fourth, the Core will assist in the transfer of technology relating to hormone measurement systems or ligand preparation to participating investigators. The accurate, precise and dependable measurement of reproductive hormones is an essential component in the investigative mission of this U54 Center. The Justification for establishing a Core facility relates to the savings in cost, resources and personnel to the U54 investigators, as they will not need to develop and maintain these methods in each individual laboratory. The technical expertise and equipment required to perform high volume or diverse testing by each investigator would be inefficient and divert effort and resources from important research questions. Also, a central Core facility can implement uniform standards for assay performance, quality controls and data reduction. The implementation of new methods or enhancement of current methods require technical expertise that would be costly and inefficient to establish in-house by each project investigator. One of the major rationales for establishing the Core is to provide state of the art assay services in a cost efficient manner. This mission will include development of assays with enhanced sensitivity and specificity, transferring methods from radioactive to nonradioactive systems, implementation of automation equipment (for high volume tests). Assay automation can enhance assay performance by improving assay precision and reducing costs by increasing the number of tests that can be performed by the technologist. Finally, the Core facility has and will continue to provide services to other U54 Centers and other reproductive scientists within the University of Virginia, promoting a spirit of cooperation that may lead to future collaborations between investigators.

配体检测和分析核心将在U54中心发挥重要作用。首先，Core将通过免疫测定法测量血液和组织样本中的肽和类固醇生殖激素。其次，开发新的最先进的检测方法，以满足U54研究者的需求。第三，制备标记的配体（肽，抗体）用于免疫测定，免疫细胞化学或结合研究。第四，核心将协助向参与的研究者转让与激素测量系统或配体制备有关的技术。 准确、精确和可靠地测量生殖激素是U54中心调查使命的重要组成部分。建立核心机构的理由与U54研究者的成本、资源和人员节省有关，因为他们不需要在每个实验室开发和维护这些方法。每个研究者进行大量或多样化测试所需的技术专长和设备将是低效的，并将精力和资源从重要的研究问题上转移。此外，中央核心设施可以实施用于测定性能、质量控制和数据简化的统一标准。实施新方法或加强现有方法需要技术专门知识，而建立这些专门知识的成本高，效率低 由每个项目调查员在内部进行。建立核心的主要理由之一是以具有成本效益的方式提供最先进的分析服务。这一使命将包括开发具有更高灵敏度和特异性的化验，将方法从放射性系统转移到非放射性系统，实施自动化设备（用于高容量测试）。分析自动化可以通过提高分析精度来增强分析性能，并通过增加技术人员可以执行的测试数量来降低成本。最后，核心设施已经并将继续为弗吉尼亚大学的其他U54中心和其他生殖科学家提供服务，促进合作精神，这可能会导致研究人员之间的未来合作。