Utilisation of iron from transferrin and lactoferrin by campylobacters

弯曲杆菌利用转铁蛋白和乳铁蛋白中的铁

• 批准号: BB/F022328/1
• 负责人: Julian Ketley
• 金额: $ 49.53万
• 依托单位: University of Leicester
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2008
• 资助国家: 英国
• 起止时间: 2008 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FF022328%2F1
• 关键词: Utilisation; iron; transferrin; lactoferrin; campylobacters

Iron is a nutrient that bacteria need for many essential processes in the cell. As part of the response to infection by bacteria, animals restrict the amount of iron available. In mammals lactoferrin (in mucosal secretions) and transferrin (in serum) tightly bind free iron to restrict its availability. Unfortunately, successful disease-causing bacteria have found ways to grab iron back from the host. The bacterial mechanisms involved in acquiring iron in the host usually consist of a specific pore on the outer surface of the bacterial cell and an associated transport system that makes the iron available inside the cell. The most important food-associated bacterium that infects humans is Campylobacter and as such is responsible for much misery and economic loss in the UK. Campylobacters normally live in the intestine of many animals, notably chickens, where they do not always cause disease. However, when humans eat food contaminated with campylobacters, an highly unpleasant cramping diarrhoea can follow. Food producers strive to minimise the risks of food poisoning for consumers, but if Campylobacter could be eliminated from the intestines of farm animals, particularly chickens, it would have a significant impact on human health by preventing many thousands of cases of food borne disease each year. If control is to be achieved it is important that we better understand how Campylobacter colonises the intestine. Several components of campylobacters that are essential for growth in the intestine are involved in acquiring iron within the host. In our preliminary work, we have identified a system in campylobacters that can grab iron directly from lactoferrin and transferrin to support bacterial growth. This work has identified candidates for the specific pore on the outer surface of the bacterial cell and the associated transport system that moves the iron to the inside of the cell. Our project objectives are to verify the identify of the components of the system and determine the methods by which iron is removed from lactoferrin/transferrin to allow bacterial growth. With a better understanding of the iron acquisition system we will be able to determine if it would be an suitable target for intervention on the farm in order to block growth in the animal gut and reduce food contamination.

铁是细菌在细胞中许多基本过程所需的营养素。作为对细菌感染的反应的一部分，动物限制了铁的可用量。在哺乳动物中，乳铁蛋白（粘膜分泌物中）和转铁蛋白（血清中）紧密结合游离铁以限制其可用性。不幸的是，成功的致病细菌已经找到了从宿主身上夺回铁的方法。在宿主体内获得铁的细菌机制通常由细菌细胞外表面上的特定孔和使铁在细胞内可用的相关运输系统组成。感染人类的最重要的食物相关细菌是弯曲杆菌，因此在英国造成了许多痛苦和经济损失。弯曲杆菌通常生活在许多动物的肠道中，特别是鸡，它们并不总是引起疾病。然而，当人类食用被弯曲杆菌污染的食物时，会出现非常不愉快的痉挛性腹泻。食品生产商努力将消费者食物中毒的风险降至最低，但如果弯曲杆菌可以从农场动物，特别是鸡的肠道中消除，它将通过预防每年数千例食源性疾病对人类健康产生重大影响。如果要实现控制，重要的是我们要更好地了解弯曲杆菌如何在肠道中定植。弯曲杆菌的几种成分对肠道中的生长至关重要，它们参与了宿主体内铁的获取。在我们的初步工作中，我们已经确定了弯曲杆菌中的一个系统，该系统可以直接从乳铁蛋白和转铁蛋白中捕获铁以支持细菌生长。这项工作已经确定了细菌细胞外表面特定孔隙的候选者以及将铁移动到细胞内部的相关运输系统。我们的项目目标是验证系统组件的识别，并确定从乳铁蛋白/转铁蛋白中去除铁以允许细菌生长的方法。通过更好地了解铁的获取系统，我们将能够确定它是否是农场干预的合适目标，以阻止动物肠道的生长并减少食品污染。

项目成果

Investigation of the function of Campylobacter jejuni glyceraldehyde-3-phosphate dehydrogenase in iron acquisition from lactoferrin

空肠弯曲杆菌甘油醛-3-磷酸脱氢酶在从乳铁蛋白中获取铁中的功能研究

• 发表时间: 2018
• 作者: Fadel Mohamed-Elamen Mohamed Mahmoud