The activation mechanism of the Bacillus subtilis stressosome signalling hub
枯草芽孢杆菌应激体信号中枢的激活机制
基本信息
- 批准号:BB/G001553/1
- 负责人:
- 金额:$ 54.64万
- 依托单位:
- 依托单位国家:英国
- 项目类别:Research Grant
- 财政年份:2008
- 资助国家:英国
- 起止时间:2008 至 无数据
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The survival of all organisms is dependent on their ability to adapt to changes in their environment and the stress response of Bacillus subtilis, and a number of its close Gram-positive relatives, provides these organisms with a protective mechanism against a wide range of physical and chemical stresses. This research proposal concerns the mechanism by which B. subtilis is able to respond to environmental stimuli. At the top of the stress-response pathway is a structure resembling a small virus that acts as a signalling hub, and has been named the stressosome. This stressosome is composed of a number of proteins that sense stress-inducing stimuli that are related to a protein called RsbR and another small protein, RsbS, that together act to sequester a protein kinase, RsbT, required to kick-start a cascade of interactions that ultimately leads to the activation of over two hundred genes whose products provide the cell with resistance to stress. The structure of the stressosome that we have recently determined, displays an unusual arrangement of protein components providing tantalising clues as to the mechanism of action in response to stress. We aim to use a blue-light responsive signalling protein to generate a stressosome that we can use in structural studies to determine any gross-structural changes in the complex that lead to the release of the protein kinase and thus the mechanism by which the stress response is initiated. Furthermore, we will investigate the role of the RsbS protein, the absence of which causes bacteria to develop as small and sickly cells, in stress-response by creating stressosomes that consist only of an RsbR-like protein. It is also apparent that the proteins related to RsbR may function as signalling modules. A full understanding of how these proteins work is dependent on the determination of their structures by X-ray crystallography, which provides a molecular model of the protein that can be used to assess potential activating signals that are recognised by their specific shapes. Finally, we will investigate the role of a number of other proteins that are known to mediate the length of the stress signal, as the requirement to turn off the stress response in a timely fashion is almost as important as the ability to respond to stress in the first place.
所有生物体的存活取决于其适应环境变化的能力,枯草芽孢杆菌及其许多革兰氏阳性近亲的应激反应为这些生物体提供了针对广泛的物理和化学应激的保护机制。这项研究计划涉及B.枯草芽孢杆菌能够对环境刺激作出反应。在应激反应途径的顶端是一个类似于小病毒的结构,它充当信号中枢,并被命名为应激体。这种应激体由许多蛋白质组成,这些蛋白质感知应激诱导刺激,这些刺激与一种称为RsbR的蛋白质和另一种小蛋白RsbS有关,它们共同作用以隔离蛋白激酶RsbT,该蛋白激酶RsbT需要启动级联相互作用,最终导致超过200个基因的激活,这些基因的产物为细胞提供抗应激能力。我们最近确定的应激体的结构显示了蛋白质组分的不寻常排列,为应激反应的作用机制提供了诱人的线索。我们的目标是使用一个蓝光响应信号蛋白,以产生一个应激体,我们可以使用在结构研究,以确定在复杂的,导致蛋白激酶的释放,从而应激反应的机制启动任何总结构的变化。此外,我们将研究RsbS蛋白的作用,该蛋白的缺乏会导致细菌发育为小而多病的细胞,通过创建仅由RsbR样蛋白组成的应激体来进行应激反应。同样明显的是,与RsbR相关的蛋白质可以作为信号模块起作用。对这些蛋白质如何工作的充分理解取决于X射线晶体学对其结构的确定,X射线晶体学提供了蛋白质的分子模型,可用于评估由其特定形状识别的潜在激活信号。最后,我们将研究一些其他已知的调节应激信号长度的蛋白质的作用,因为及时关闭应激反应的要求几乎与首先应对应激的能力一样重要。
项目成果
期刊论文数量(6)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Simulations of stressosome activation emphasize allosteric interactions between RsbR and RsbT.
- DOI:10.1186/1752-0509-7-3
- 发表时间:2013-01-15
- 期刊:
- 影响因子:0
- 作者:Liebal UW;Millat T;Marles-Wright J;Lewis RJ;Wolkenhauer O
- 通讯作者:Wolkenhauer O
The Vibrio vulnificus stressosome is an oxygen-sensor involved in regulating iron metabolism.
- DOI:10.1038/s42003-022-03548-w
- 发表时间:2022-06-27
- 期刊:
- 影响因子:5.9
- 作者:
- 通讯作者:
The Bacillus subtilis stressosome: A signal integration and transduction hub.
枯草芽孢杆菌应激体:信号整合和转导中心。
- DOI:10.4161/cib.1.2.7225
- 发表时间:2008
- 期刊:
- 影响因子:0
- 作者:Marles-Wright J
- 通讯作者:Marles-Wright J
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Richard Lewis其他文献
The C@merata task at MediaEval 2016: Natural Language Queries Derived from Exam Papers, Articles and Other Sources against Classical Music Scores in MusicXML
MediaEval 2016 上的 C@merata 任务:针对 MusicXML 中的古典音乐乐谱从试卷、文章和其他来源导出的自然语言查询
- DOI:
- 发表时间:
2016 - 期刊:
- 影响因子:0
- 作者:
R. Sutcliffe;Tom Collins;E. Hovy;Richard Lewis;C. Fox;Deane L. Root - 通讯作者:
Deane L. Root
Exploring information retrieval, semantic technologies and workflows for music scholarship: the Transforming Musicology project
探索音乐学术的信息检索、语义技术和工作流程:转变音乐学项目
- DOI:
- 发表时间:
2015 - 期刊:
- 影响因子:0
- 作者:
Richard Lewis;T. Crawford;David Lewis - 通讯作者:
David Lewis
Parental Occupation and the Gender Math Gap: Examining the Social Reproduction of Academic Advantage among Elementary and Middle School Students
父母职业与性别数学差距:审视中小学生学业优势的社会再生产
- DOI:
10.3390/socsci7010006 - 发表时间:
2017 - 期刊:
- 影响因子:0
- 作者:
Monica Bowden;J. Bartkowski;Xiaohe Xu;Richard Lewis - 通讯作者:
Richard Lewis
NP36 - Using the Child as Change Agent for Preventing Childhood Obesity in Rural Georgia: Home Environment Results
- DOI:
10.1016/j.jneb.2018.04.272 - 发表时间:
2018-07-01 - 期刊:
- 影响因子:
- 作者:
Marsha Davis;Courtney Brown;Andrea Scarrow;Frances McCarty;Richard Christiana;Richard Lewis;Rebecca Mullis - 通讯作者:
Rebecca Mullis
Artificial intelligence in chemistry and drug design
- DOI:
10.1007/s10822-020-00317-x - 发表时间:
2020-05-29 - 期刊:
- 影响因子:3.100
- 作者:
Nathan Brown;Peter Ertl;Richard Lewis;Torsten Luksch;Daniel Reker;Nadine Schneider - 通讯作者:
Nadine Schneider
Richard Lewis的其他文献
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{{ truncateString('Richard Lewis', 18)}}的其他基金
NSF-SSRC: Reducing Vaccine Hesitancy Through Interactive Decision Aids
NSF-SSRC:通过交互式决策辅助工具减少疫苗犹豫
- 批准号:
2241963 - 财政年份:2023
- 资助金额:
$ 54.64万 - 项目类别:
Continuing Grant
Is the GpsB:PBP1 interaction an Achilles' heel for Gram-positive pathogens?
GpsB:PBP1 相互作用是革兰氏阳性病原体的致命弱点吗?
- 批准号:
BB/R012520/1 - 财政年份:2018
- 资助金额:
$ 54.64万 - 项目类别:
Research Grant
Architecture of the bacterial divisome.
细菌分裂体的结构。
- 批准号:
BB/M001180/1 - 财政年份:2015
- 资助金额:
$ 54.64万 - 项目类别:
Research Grant
Phosphotransferases in bacterial cell wall biosynthesis
细菌细胞壁生物合成中的磷酸转移酶
- 批准号:
BB/J015016/1 - 财政年份:2012
- 资助金额:
$ 54.64万 - 项目类别:
Research Grant
Language Processing as Boundedly Optimal Control of Memory, Perception, and Action
语言处理作为记忆、感知和行动的有限最优控制
- 批准号:
1152819 - 财政年份:2012
- 资助金额:
$ 54.64万 - 项目类别:
Continuing Grant
Modelling carbon core metabolism in Bacillus subtilis - exploring the contribution of protein complexes in core carbon and nitrogen metabolism
模拟枯草芽孢杆菌的碳核心代谢 - 探索蛋白质复合物在核心碳和氮代谢中的贡献
- 批准号:
BB/I004572/1 - 财政年份:2010
- 资助金额:
$ 54.64万 - 项目类别:
Research Grant
Functional studies of the stressosome
应激体的功能研究
- 批准号:
BB/F001533/1 - 财政年份:2008
- 资助金额:
$ 54.64万 - 项目类别:
Research Grant
Structural analysis of the interaction networks
交互网络的结构分析
- 批准号:
BB/F003404/1 - 财政年份:2007
- 资助金额:
$ 54.64万 - 项目类别:
Research Grant
RUI: Establishment of a high-density event-related potential laboratory at an undergraduate college for the study of cognitive neuroscience
RUI:在本科院校建立高密度事件相关电位实验室,进行认知神经科学研究
- 批准号:
0116836 - 财政年份:2001
- 资助金额:
$ 54.64万 - 项目类别:
Standard Grant
Laboratory Equipment to Improve Undergraduate Instruction inPhysiological Psychology
改善生理心理学本科教学的实验室设备
- 批准号:
8750744 - 财政年份:1987
- 资助金额:
$ 54.64万 - 项目类别:
Standard Grant
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