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BsrE/SR5: a novel type I toxin-antitoxin system in Bacillus subtilis: Localization of the toxin and identification of its cellular target(s), mechanism(s) of antitoxin action, and biological functions of the system

BsrE/SR5：枯草芽孢杆菌中的新型 I 型毒素-抗毒素系统：毒素的定位及其细胞靶标的鉴定、抗毒素作用机制以及系统的生物学功能

基本信息

批准号：
276973612
负责人：
Privatdozentin Dr. Sabine Brantl
金额：
--
依托单位：
Lehrstuhl für Genetik
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2015
资助国家：
德国
起止时间：
2014-12-31 至 2019-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/276973612?language=en
关键词：
BsrE SR5 novel type toxin

项目摘要

Toxin/antitoxin systems (TA systems) are pairs of adjacent genes, one of which encodes a stable toxic peptide that is neutralized by an unstable antitoxin coded by the neighbouring gene. Currently, 5 types of TA systems are known. In type I TA systems, the antitoxin is a small regulatory RNA, which interacts with the toxin mRNA, and interaction results in inhibition of toxin translation or/and degradation of the toxin mRNA. Whereas the function of plasmid-encoded TA systems has been investigated and elucidated a long time ago (they are post-segregational killing systems, i.e. they cause the death of plasmid free cells), the biological function and importance of chromosomal type I TA systems is still a matter of speculation. In this project, we plan to investigate a new chromosome-encoded type I TA system from Bacillus subtilis, bsrE/SR5, with regard to three aspects: 1) Cellular localization of the toxic peptide, its cellular target(s) and its/their biochemical activities. As a novelty, we will use cutting-edge techniques from cell biology to identify toxin targets. 2) Mechanism of action of the RNA antitoxin 3) Biological function/importance of this system for Bacillus subtilis.

毒素/抗毒素系统（TA系统）是一对相邻的基因，其中一个编码一个稳定的毒性肽，该肽被相邻基因编码的不稳定的抗毒素中和。目前，已知5种类型的TA系统。在I型TA系统中，抗毒素是小的调节RNA，其与毒素mRNA相互作用，并且相互作用导致毒素翻译的抑制或/和毒素mRNA的降解。尽管质粒编码的TA系统的功能在很久以前就已经被研究和阐明（它们是分离后杀伤系统，即它们引起无质粒细胞的死亡），但染色体I型TA系统的生物学功能和重要性仍然是推测的问题。本课题主要从三个方面对枯草芽孢杆菌（Bacillussubtilis）染色体编码的新型Ⅰ型TA系统bsrE/SR 5进行研究：1）毒性肽的细胞定位、细胞靶点及其生物活性。作为一项新奇，我们将使用细胞生物学的尖端技术来识别毒素目标。2)RNA抗毒素的作用机制3）该系统对枯草芽孢杆菌的生物学功能/重要性。