Long Term Storage and Precision Dispensing of Precious Liquids

贵重液体的长期储存和精确分配

• 批准号: 7496476
• 负责人: GORDON Ian ROBERTSON
• 金额: $ 42.19万
• 依托单位: ARCHIVEX, LLC
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7496476
• 关键词: Aliquot; Archives; Area; Arts; Biological Sciences; Characteristics; Collaborations; Complement; Complementary DNA; Complex; Computer software; DNA; Data; Databases; Development; Disadvantaged; Ensure; Equipment; Equipment and supply inventories; Forensic Medicine; Frequencies; Funding; Genetic; Genomics; Genotype; Goals; Injection of therapeutic agent; Label; Laboratories; Laboratory Research; Lead; Liquid substance; Manuals; Marketing; Methods; Molds; Operative Surgical Procedures; Performance; Phase; Plastics; Preparation; Prevention; Price; Printing; Process; Productivity; Proteins; Public Health; Qualifying; Rare Diseases; Rate; Research; Research Personnel; Resistance; Resources; Risk; Robot; Sampling; Secure; Site; Solid; Source; Standards of Weights and Measures; Sterilization for infection control; System; Technology; Testing; Time; Touch sensation; Update; aqueous; base; cost; density; design; disease phenotype; evaporation; experimental analysis; hospital laboratories; human disease; improved; innovation; microbial alkaline proteinase inhibitor; novel; prospective; prototype; research study; sample collection; user-friendly

DESCRIPTION (provided by applicant): Precious liquid samples such as aqueous genomic DNA are the bedrock for immense efforts currently underway to better understand associations between disease phenotype and genotype. These efforts promise to pave the way to better prevention and treatment of human disease. Many laboratories maintain archives of thousands of such precious samples for years at a time while they pursue better understanding of both common and rare diseases. Current methods for managing precious liquid samples are fraught with problems, including poor inventory control, poor ability to search inventory without directly accessing samples, constant sample concentration changes due to evaporation, excessive time required to aliquot samples, and samples becoming lost, mislabeled, or contaminated with significant frequency. The goal of this project is to develop and commercialize a system for long term storage of precious liquids that eliminates or significantly reduces all of these problems. This system stores liquids in standardized containers with machine-readable tags for unambiguous identification. An automated precision dispenser which does not require pipette intrusion ensures sample integrity while dispensing microliter aliquots of precious liquid. An interface between the dispensing equipment and an archived-liquid database application will allow laboratories to conveniently maintain accurate inventory control and facilitate improved experiment planning. This workflow-based system will increase the efficiency and reduce the error rate of genomic research by ensuring that the precious liquid samples are less time consuming to inventory and access, are contamination-free, maintain their concentration levels, and are correctly and positively identified. When completed, the system will be commercialized by marketing it to life- science laboratories, forensic laboratories and hospitals. In Phase 1 of this project, key inventions were built and tested with excellent results proving feasibility: (1) the compact long-term storage and dispensing container maintains precious liquid in an evaporation and contamination resistant manner; (2) the user-friendly dispensing mechanism can deliver 1-1500¿l aliquots touch-free with state-of-the-art precision; and (3) a user- friendly archived-liquid sample tracking database application was planned with the input of prospective customers using Agile Software methods. During Phase 2 of the project, the technology risk of manufacturing this novel liquid storage container with unique and permanent identification will be reduced (Specific Aim 1), commercial versions of the semi- and fully-automatic Precision Dispensing System will be designed and built (Specific Aim 2), and the workflow-based liquid sample tracking database will be built and integrated with the storage cartridges and precision dispensing equipment (Specific Aim 3).These early versions of the commercial equipment will be Beta tested in a nationally known genetics core resource laboratory. During Phase 3 using other funds this fully developed novel system will be introduced to the commercial marketplace. Research laboratories in the U.S. and worldwide store thousands of precious liquid DNA samples but use inefficient and error-prone ways to manage them. Archivex is developing an integrated system for storing, dispensing and inventorying precious liquids which will be convenient and affordable, will pay for itself through reduced labor costs, and will lead to improved public health by increasing the productivity and effective collaboration of laboratories. The hardware will ensure the integrity of the stored liquids and enable contamination-free precision dispensing, while the database application will allow researchers to better take advantage of their full sample collection, including more efficient analysis of experimental outliers

描述（由适用提供）：诸如水性基因组DNA之类的宝贵液体样品是目前正在进行的巨大努力的基石，以更好地了解疾病表型与基因型之间的关联。这些努力有望为更好的预防和治疗人类疾病铺平道路。许多实验室在多年来都保留了数千个这样的宝贵样本的档案，同时他们对常见和罕见疾病都有更好的了解。当前用于管理宝贵液体样品的方法是有问题的，包括库存控制差，搜索库存的能力差而无需直接访问样品，由于蒸发而导致的持续样品浓度变化，等分试样样品所需的时间过多以及样本变得丢失，丢失，标签错误或污染了很大的频率。该项目的目的是开发和商业化一种用于长期存储珍贵液体的系统，以消除或大大减少所有这些问题。该系统将液体存储在标准化容器中，并带有可读的机器可读标签，以进行明确的识别。不需要移液侵入的自动精度分配器可确保样品完整性，同时分配珍贵液体的微量液等分试样。分配设备与存档数据库应用程序之间的接口将使实验室可以方便地维护准确的库存控制并促进改进的实验计划。这种基于工作流的系统将通过确保珍贵的液体样品减少库存和获取的时间来提高效率并降低基因组研究的错误率，无污染，保持其浓度水平，并正确鉴定出来。完成后，该系统将通过向生命科学实验室，法医实验室和医院进行营销。在该项目的第一阶段中，建立和测试了主要的发明，并提供了可行性的出色结果：（1）紧凑的长期存储和分配容器在经济和抵抗污染的方式中保持宝贵的液体； （2）用户友好的分配机制可以通过最先进的精度提供1-1500 liefle-Feal-Fire触摸； （3）计划使用敏捷软件方法的潜在客户的输入来计划用户友好的存档示例跟踪数据库应用程序。在该项目的第二阶段中，将减少制造具有独特和永久识别的新型液体存储容器的技术风险（特定AIM 1），在全国已知的遗传学核心资源实验室中测试了beta，将在商业设备的早期版本中进行半自动和完全自动的精确分配系统的商业版本。在第3阶段使用其他资金的情况下，该全面开发的新型系统将被引入商业市场。美国和全球的研究实验室存储了数千种宝贵的液体DNA样品，但使用无效且容易出错的方法来管理它们。 Archivex正在开发一种集成系统，用于存储，分配和库存珍贵的液体，这些液体将方便且负担得起，将通过降低的人工成本来为自己付费，并通过提高实验室的生产力和有效的协作来改善公共卫生。硬件将确保储存的液体的完整性并实现无污染的精度分配，而数据库应用程序将使研究人员能够更好地利用其完整的样品收集，包括对实验异常值的更有效分析