Cell, Animal and Imaging Core

细胞、动物和成像核心

• 批准号: 7728884
• 负责人: THERESA A GUISE
• 金额: $ 12.23万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7728884
• 关键词: American Type Culture Collection; Animal Experiments; Animal Model; Animals; Behavior; Biological Assay; Bladder; Bone neoplasms; Cell Line; Cell physiology; Cells; Cellular Structures; Common Neoplasm; Cultured Cells; Data; Data Quality; Databases; Detection; Documentation; Endothelial Cells; Equipment; Fingerprint; Firefly Luciferases; Freezing; Genetic screening method; Goals; Guidelines; Histology; Housing; Human Cell Line; Human Resources; Image; Image Analysis; Injection of therapeutic agent; Instruction; Laboratories; Lead; Life; Luciferases; Malignant neoplasm of prostate; Manuscripts; Metastatic Neoplasm to the Bone; Microscopy; Modality; Morphology; Mus; Mycoplasma; Nature; Neoplasm Metastasis; Orchiectomy; Organ Culture Techniques; Osteoblasts; Osteoclasts; Osteolytic; Phenotype; Procedures; Process; Prostate; Reagent; Reporter; Research; Research Personnel; Resources; Risk; Sampling; Source; Standardization; Standards of Weights and Measures; System; Techniques; Testing; Tissues; Training Programs; Tumor-Derived; Xenograft Model; Xenograft procedure; angiogenesis; base; bone; experience; immortalized cell; improved; in vivo; macromolecule; member; mouse model; neoplastic cell; programs; promoter; repository; single cell analysis; subcutaneous; tissue culture; tumor

The Program makes extensive use of human cell lines and animal models of prostate cancer. The overall goal of this Core is to provide materials, instructional support, technical aid,/n vivo imaging of mouse models of prostate cancer metastases, and ex-vivo assays of tumor-bone interactions to Project investigators. This will encompass the storage and distribution of well-characterized prostate cell lines and xenografts; the storage and distribution of reporter and inducible cell lines; and instruction, guidelines and assistance with xenograft studies, Xenogen and Faxitron imaging and assays for osteoblast, osteoclast and angiogenesis. Such standardization of cell culture reagents and animal techniques will facilitate research by improving the quality of the data, reducing redundancy and allowing comparison of results between the all Projects. This Core will have 5 Aims: 1) Provide wellcharacterized prostate cancer and normal prostate cell lines to investigators and house a repository of all common tumor and immortalized cell lines. In addition, the Core will carry out mycoplasma testing and genetic fingerprinting (SNP analysis and/or VNTRs) of all cell lines received by Program investigators from outside sources. Thus, any cell line received by an investigator will first be processed by the Core in this way and a sample frozen in the repository. The Core will also serve as a repository of new cell lines derived by Program investigators such as those with inducible promoters (S. Parsons, Weber) or with imaging capability such as those containing transfected luciferase constructs (Theodorescu). 2) Provide live instruction and guidelines for investigators using xenograft models such as those derived after subcutaneous, intracardiac, intrafemoral and intraprostatic injection of tumor cells and with procedures such as orchiectomy. Instructional videos will be made of these procedures and saved on a central server database (PROSTA, Core A) accessible by all Program members via standard networked PCs. 3) Support for in vivo fluorescent imaging of tumors and Faxitron radiographic imaging and quantitation of bone metastases will be provided by the Core. Technical help will involve providing instruction as well as taking animals to the UVA SAMMIC core for MS (Xenogen Corp.) imaging of tumors expressing luciferase. 4) Assessment of tumor-bone interactions will be performed using wellcharacterized assays of osteoblast, osteoclast and endothelial cell function. 5) Microscopy Imaging: a) Resources for the routine analysis and documentation of cell structure and morphology and the detection of macromolecules in fixed, live cells and tissues; b) resources for single cell analysis; and c) training for Program personnel and overall oversight of Core equipment.

该计划广泛使用人类细胞系和前列腺癌的动物模型。的总目标是 这个核心是提供材料，教学支持，技术援助，/n活体成像的小鼠模型的前列腺 癌症转移，以及项目研究人员肿瘤-骨相互作用的体外分析。这将包括 特性良好的前列腺细胞系和异种移植物的储存和分配； 报告和诱导细胞系；以及异种移植研究的指导、指南和协助，Xenogen和 Faxitron成像和成骨细胞、破骨细胞和血管生成的检测。这种细胞培养的标准化 试剂和动物技术将通过提高数据质量、减少冗余来促进研究 以及允许比较所有项目之间的结果。此核心将有5个目标：1)提供良好的特性 前列腺癌和正常前列腺细胞系的研究人员和所有常见的 肿瘤和永生化细胞系。此外，核心将进行支原体测试和基因 项目调查员从外部收到的所有细胞系的指纹(SNP分析和/或VNRR) 消息来源。因此，由调查员接收的任何细胞系将首先由核心以这种方式进行处理，并且 保存在储存库中的样本。Core还将作为由Program派生的新细胞系的储存库 具有诱导性启动子的研究人员(S.Parsons，Weber)或具有成像能力的研究人员 含有转基因的荧光素酶构建体(Theodresu)。2)提供现场指导和指导 研究人员使用异种移植模型，如皮下、心内、股内和 前列腺内注射肿瘤细胞，并配合切除术等程序。将制作教学视频 并保存在所有程序均可访问的中央服务器数据库(PROSTA，核心A)上 会员通过标准的联网PC。3)支持肿瘤和Faxitron的体内荧光成像 骨转移的放射成像和定量将由Core提供。技术帮助将涉及 为MS(Xenogen Corp.)提供指导并将动物带到UVA SAMMIC核心。成像 表达荧光素酶的肿瘤。4)将使用Well Characteristic进行肿瘤-骨相互作用的评估 成骨细胞、破骨细胞和内皮细胞功能检测。5)显微成像：A)资源 用于细胞结构和形态的常规分析和记录以及大分子的检测 在固定、活细胞和组织方面；b)单细胞分析资源；c)培训方案人员和 全面监督核心设备。