Histology/Immunohistology

组织学/免疫组织学

• 批准号: 7728855
• 负责人: DAVID V GOLD
• 金额: $ 6.58万
• 依托单位: CTR FOR MOLECULAR MEDICINE/IMMUNOLOGY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7728855
• 关键词: Acetic Acid; Acetic Acids; Address; Aftercare; Aliquot; Animals; Antibodies; Antigen-Presenting Cells; Antigens; Apoptosis; Applications Grants; Avidin; B lymphoid malignancy; B-Cell Neoplasm; B-Lymphocytes; Binding; Biological Assay; Biological Markers; Biological Preservation; Biotin; Bispecific Antibodies; Blood; Blood Platelets; Blood specimen; Budgets; Buffers; CD19 gene; CD22 antigen; CD22 gene; Carbon Dioxide; Cell Fraction; Cell Line; Cell Lineage; Cells; Centrifugation; Characteristics; Class; Clinical; Clinical Pathology; Collection; Colon Carcinoma; Color; Computer Assisted; Computer software; Cooperative Human Tissue Network; Core Facility; Cultured Cells; Cytolysis; Data; Dendritic Cells; Densitometry; Digestion; Disruption; Drug or chemical Tissue Distribution; Emulsions; Epithelial Cells; Epitopes; Epratuzumab; Equipment; Equus caballus; Erythrocytes; Ethanol; Exhibits; Ficoll; Fixatives; Flow Cytometry; Fluorescein-5-isothiocyanate; Formalin; Fox Chase Cancer Center; Freezing; Fresh Water; Frozen Sections; Genus Capra; Glass; Glycerol; Goat; Gold; Growth; HLA-DR Antigens; Hairy Cell Leukemia; Haptens; Heating; Hematoxylin; Hematoxylin and Eosin Staining Method; Heterogeneity; High temperature of physical object; Histologic; Histology; Hospitals; Housing; Hydrogen Peroxide; Image; Immunoassay; Immunoglobulin G; Immunoperoxidase Technics; Incubated; Incubators; Individual; Institution; Label; Laboratories; Light; Light Microscope; Link; Liquid substance; Lung; Lymphocyte; Lymphoid; Lymphoma; MS4A1 gene; Malignant - descriptor; Malignant Neoplasms; Manufacturer Name; Marrow; Mature B-Lymphocyte; Measures; Mechanics; Methanol; Methods; Microscopy; Microtome - medical device; Modification; Molecular; Monoclonal Antibodies; Morphology; Mucin-1 Staining Method; Multiple Myeloma; Mus; Myelogenous; N-hydroxysuccinimide; Normal Horse Serum; Normal tissue morphology; Numbers; Operative Surgical Procedures; Osmotic Shocks; Paraffin Embedding; Paraffin Tissue; Patients; Pattern; Performance; Peroxidase; Peroxidases; Population; Preparation; Procedures; Program Research Project Grants; Program Reviews; Protocols documentation; Purpose; Radioimmunoconjugate; Radioimmunotherapy; Radiolabeled; Range; Reagent; Relative (related person); Reporting; Research Personnel; Retrieval; Rhodamine; Rhodamines; Roswell Park Cancer Institute; SCID Mice; Serum; Services; Slide; Sodium Chloride; Solid Neoplasm; Solutions; Source; Specimen; Staining method; Stains; Stem cells; Structure of thyroid parafollicular cell; Study Section; Sum; Surveys; Suspension substance; Suspensions; Techniques; Technology; Temperature; Testing; Time; Tissue Banks; Tissue Embedding; Tissue Procurements; Tissue Stains; Tissues; Tracer; Transcriptional Activation; Tube; Tumor Markers; Up-Regulation; Water; Week; Work; Xenograft procedure; animal tissue; cancer type; charge coupled device camera; collagenase; cryogenics; cryostat; digital; fluorescence microscope; follow-up; granulocyte; instrument; interest; invariant chain; kidney cell; light microscopy; macrophage; member; microautoradiography; monoclonal antibody LL2; monocyte; neoplastic cell; paraform; peripheral blood; plasma cell differentiation; precursor cell; pressure; programs; radiotracer; rituximab; sarcoma; sodium phosphate; tissue fixing; tositumomab; tumor; vector

CMMI currently maintains an active Histology Laboratory that has provided various histologic staining imtnunoassay procedures as a service for in-house investigators. This Core Facility will be responsible for tissue procurement, preparation, sectioning, and the performance of immunoenzyme and immunofluorescent assays, microautoradiography, and specialized histochemical procedures. Examination of stained specimens can be carried out by light microscopy, fluorescent microscopy or video densitometer. For the purposes of this grant proposal Dr. Gold, (Core Leader) will review all slides. Additional assistance in the review of stained slides is available from our collaborators in this Program Project, Drs. Ely and Knowles from NY Hospital (NYC, NY). A majority of the specimens for histology services will be provided by members of the Program Project, either from experimental animal and cell culture studies or clinical pathology material to be obtained from Drs. Ely and Knowles of the NY Hospital (NYC, NY). As a yearly average we expect 1250 specimens a year for histology services.

CMMI目前拥有一个活跃的组织学实验室，提供各种组织学染色免疫测定 作为一项服务，为内部调查人员。该核心机构将负责组织采购， 制备、切片和免疫酶和免疫荧光测定的性能，显微放射自显影， 和专门的组织化学程序。染色标本的检查可通过光学显微镜进行， 荧光显微镜或视频密度计。为了这项赠款提案的目的，黄金博士（核心领导人）将 查看所有幻灯片。我们的合作者可在此项目中提供染色切片审查方面的额外帮助 项目，来自纽约医院（NYC，NY）的伊利和诺尔斯博士。大部分用于组织学服务的标本将 由计划项目的成员提供，无论是从实验动物和细胞培养研究或临床 病理学材料从纽约医院（NYC，NY）的伊利和诺尔斯博士处获得。平均每年，我们 预计每年有1250个标本用于组织学服务。