Detection and Diagnosis of Pancreatic Carcinoma

胰腺癌的检测和诊断

• 批准号: 8465742
• 负责人: DAVID V GOLD
• 金额: $ 24.82万
• 依托单位: CTR FOR MOLECULAR MEDICINE/IMMUNOLOGY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-09 至 2015-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8465742
• 关键词: Abdominal Pain; Affinity; Antibodies; Antibody Specificity; Antigens; Benign; Biochemical; Biological Assay; Biological Markers; Blood; Body Weight decreased; Cancer Etiology; Carcinoma; Cessation of life; Clinical; Clinical Trials; Data; Data Correlations; Detection; Development; Diagnosis; Diagnostic; Discrimination; Disease; Disease Progression; Early Diagnosis; Enzyme Immunoassay; Epitopes; Evaluation; Funding; Generations; Glycoproteins; Goals; Grant; Hepatobiliary; Histocompatibility Testing; Histologic; Icterus; Image; Immunoassay; In Vitro; Individual; Investigation; Laboratories; Lesion; Liquid substance; Location; Malignant - descriptor; Malignant Neoplasms; Malignant neoplasm of pancreas; Mass Spectrum Analysis; Measures; Medical Assistance; Mind; Modification; Monoclonal Antibodies; Mucins; Mus; Nature; Normal tissue morphology; Observational Study; Operative Surgical Procedures; Outcome; Pancreas; Pancreatic Diseases; Pancreatic Intraepithelial Neoplasia; Pancreatic carcinoma; Pancreatitis; Patients; Peptides; Performance; Phage Display; Population; Predictive Value; Procedures; Property; Publications; Publishing; Relapse; Reporting; Research; Research Proposals; Role; Sampling; Serum; Specificity; Specimen; Staging; Study Section; Surveillance Program; Symptoms; Systemic Therapy; Testing; Therapeutic Intervention; Time; Tissues; Translating; United States; Woman; Work; base; cancer diagnosis; cell type; diagnostic accuracy; disease diagnosis; follow-up; high risk; improved; men; mortality; outcome forecast; pancreatic neoplasm; programs; prospective; public health relevance; response; screening; statistics; tumor; tumor growth

DESCRIPTION (provided by applicant): The objective of this research proposal is the development and evaluation of immunoassays having clinical utility for the "early" detection and diagnosis of pancreatic carcinoma. In the year 2009, an estimated 42,000 new cases of pancreatic carcinoma will be diagnosed in the United States. Pancreatic carcinoma is the tenth most common form of cancer in men and women today, yet it is the fourth leading cause of cancer deaths. The most common symptoms of the disease, jaundice, abdominal pain, and weight loss, together with other presenting factors are nonspecific in nature. Thus, diagnosing pancreatic carcinoma at an early stage of tumor growth is difficult at best, requiring considerable suspicion and extensive diagnostic work-up, up to and including exploratory surgery. Our laboratory has developed and characterized the PAM4 monoclonal antibody (murine, chimeric and humanized versions), providing evidence as to its potential for clinical detection, imaging and therapy of pancreatic carcinoma. Our recent developments employing an in vitro enzyme immunoassay to quantitate PAM4-reactive antigen in the blood of patients, appears quite promising for detection of pancreatic carcinoma and its discrimination from pancreatitis, as well as other cancers and normal individuals. Early detection and diagnosis of pancreatic carcinoma, as well as appropriate staging of the disease, would almost certainly provide a survival advantage. With this in mind, we intend to further develop and assess the ability of the PAM4-based immunoassays, both immunohistochemical detection in tissue specimens and enzyme immunoassay to detect and quantitate the antigen in both serum and pancreatic fluids, to provide "early" detection and diagnosis at a time-point when therapeutic intervention may have better opportunity for successful outcome. The specific aims of this project are: 1. To evaluate the performance profile of the PAM4-Immunoassay for diagnostic accuracy; 2. To evaluate the clinical utility of the PAM4-Immunoassay for a-accurate diagnosis of patients presenting with symptoms suggestive of pancreatic cancer; b- early detection of pancreatic carcinoma in asymptomatic individuals at high risk for pancreatic cancer; c- assessment of tumor response or early detection of relapse; 3. To evaluate the nature of the antigen to which PAM4 is reactive.

描述（由申请人提供）：本研究计划的目的是开发和评估具有临床实用性的免疫测定法，用于“早期”检测和诊断胰腺癌。 2009年，美国估计将诊断出42,000例新的胰腺癌病例。胰腺癌是当今男性和女性中第十常见的癌症，但它是癌症死亡的第四大原因。该疾病最常见的症状、黄疸、腹痛和体重减轻以及其他表现因素本质上是非特异性的。因此，在肿瘤生长的早期阶段诊断胰腺癌充其量是困难的，需要相当多的怀疑和广泛的诊断检查，直至并包括探查手术。我们的实验室开发并表征了 PAM4 单克隆抗体（鼠源、嵌合和人源化版本），为其用于胰腺癌临床检测、成像和治疗的潜力提供了证据。我们最近的发展采用体外酶免疫测定法来定量患者血液中的 PAM4 反应性抗原，对于检测胰腺癌及其与胰腺炎以及其他癌症和正常个体的区分似乎很有希望。胰腺癌的早期发现和诊断以及疾病的适当分期几乎肯定会提供生存优势。考虑到这一点，我们打算进一步开发和评估基于 PAM4 的免疫测定的能力，包括组织标本中的免疫组织化学检测和酶免疫测定来检测和定量血清和胰液中的抗原，以便在治疗干预可能有更好的机会获得成功结果的时间点提供“早期”检测和诊断。该项目的具体目标是： 1. 评估 PAM4-Immunoassay 的性能概况以实现诊断准确性； 2. 评估PAM4免疫测定法对出现胰腺癌症状的患者进行准确诊断的临床实用性； b- 在胰腺癌高危无症状个体中早期发现胰腺癌； c-评估肿瘤反应或早期检测复发； 3.评价与PAM4反应的抗原的性质。