ROLE OF CU, ZN SOD IN A. ACTINOMYCETEMCOMITANS

铜、锌、SOD 在伴放线菌中的作用

• 批准号: 7413259
• 负责人: Nataliya V Balashova
• 金额: $ 6.24万
• 依托单位: UNIV OF MED/DENT OF NJ-NJ DENTAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7413259
• 关键词: Actinobacillus actinomycetemcomitans; Antibodies; Bacteria; Binding; Cells; Class; Clinical; Cuprozinc Superoxide Dismutase; Drug Metabolic Detoxication; Environment; Enzymes; Erythrocytes; Family; Goals; Heme; Heme Iron; Human; In Vitro; Infective endocarditis; Inflammatory; Knowledge; Lead; Leukocytes; Localized; Pathogenesis; Periodontitis; Physiology; Play; Production; Proteins; Reactive Nitrogen Species; Reactive Oxygen Species; Role; Superoxide Dismutase; Superoxides; Systemic disease; Virulence; Virulence Factors; Work; antimicrobial; bactericide; extracellular; factor A; free radical oxygen; in vivo; killings; leukotoxin; mutant; oral pathogen; oxidation; pathogen; research study

Actinobacillus actinomycetemcomitans is a Gram-negative oral pathogen that is the etiologic agent of localized aggressive periodontitis. This bacterium can also cause several systemic diseases including infective endocarditis. As one of virulence factors, A. actinomycetemcomitans produces leukotoxin that kills human leukocytes and can also destroy erythrocytes. Our recent work strongly suggests that leukotoxin interacts with Cu, Zn superoxide dismutase (Cu, Zn SOD) from A. actinomycetemcomitans. Bacterial Cu, Zn SODs have a role in protecting pathogens from the oxidative damage caused by host inflamatory cells. Cu, Zn SOD has also been shown to bind heme and potentially play a role in iron and heme acquisition by bacteria. The proposed studies seek to understand the role of Cu, Zn SOD in A. actinomycetemcomitans. 1. I will study the interaction between Cu, Zn SOD and leukotoxin from A. actinomycetemcomitans. 2. I will characterize Cu, Zn SOD function in A. actinomycetemcomitans. This study will expand our knowledge of the physiology and pathogenesis of A. actinomycetemcomitans and may lead to a better understanding of the interplay between different putative virulence factors of an important oral pathogen.

Actinobacillus actinomycetemcomitans 是一种革兰氏阴性口腔病原体，是下列疾病的病原体： 局部侵袭性牙周炎。这种细菌还会引起多种全身性疾病，包括 感染性心内膜炎。作为毒力因子之一，A. actinomycetemcomitans 产生的白细胞毒素可杀死 人类白细胞，也可以破坏红细胞。我们最近的工作强烈表明白细胞毒素 与来自 A. actinomycetemcomitans 的 Cu, Zn 超氧化物歧化酶 (Cu, Zn SOD) 相互作用。细菌铜、锌 SOD 具有保护病原体免受宿主炎症细胞引起的氧化损伤的作用。铜, Zn SOD 还被证明可以结合血红素，并可能在铁和血红素的获取中发挥作用 细菌。 拟议的研究旨在了解 Cu、Zn SOD 在 A. actinomycetemcomitans 中的作用。 1. 我将研究 Cu、Zn SOD 与来自 A. actinomycetemcomitans 的白细胞毒素之间的相互作用。 2. 我将表征 A. actinomycetemcomitans 中的 Cu、Zn SOD 功能。 这项研究将扩大我们对 A. actinomycetemcomitans 的生理学和发病机制的了解， 可能会导致更好地理解不同假定毒力因子之间的相互作用 重要的口腔病原体。