Characterization of a Kingella kingae leukotoxin

Kingella kingae 白细胞毒素的表征

基本信息

项目摘要

DESCRIPTION (provided by applicant): Kingella kingae is a fastidious gram-negative coccobacillus of the Neisseriaceae family and is a normal inhabitant of the human oropharyngeal flora. This bacterium belongs to HACEK group and can cause infective endocarditis. Recently, as the result of improved isolation and identification techniques, an increasing number on invasive K. kingae infections have been reported throughout the world, suggesting that the organism is an important cause of heart and skeletal infections in pediatric and adult patients. Despite the emerging body of information on the clinical and diagnostic aspects of K.kingae infections, the virulence mechanisms of this pathogen remain largely unknown. Our results suggest that K. kingae produces a potent proteinaceious leukotoxin (Ktx) that is associated with cell membranes. This is the first evidence of a K. kingae leukotoxin that may be an important virulence factor for evasion of the host immune response during infection. The goal of this research is identify and characterize Ktx. In the proposed research we will: - Study distribution of Ktx in different K. kingae clinical isolates; - Sequence K. kingae genome; - Purify Ktx from K. kingae followed by the protein identification; - Identify ktx gene and generate ktx mutants using random and site-directed mutagenesis approaches; - Characterize Ktx toxicity to different human cells. Identification of K. kingae virulence factors will be essential to understanding how the pathogen causes disease. The results obtained in this study will have significance for the prevention and treatment of K. kingae infections. PUBLIC HEALTH RELEVANCE: Kingella kingae is a human oral bacterium that can cause infections of heart and skeletal system. We propose to characterize K. kingae toxin that can destroy human white blood cells. The results obtained in this study will have significance for the prevention and treatment of K. kingae infections.
描述(由申请人提供):Kingella kingae是奈瑟氏球菌科的一种苛养革兰氏阴性球杆菌,是人类口咽植物群的正常居民。该细菌属于HACEK组,可引起感染性心内膜炎。近年来,随着分离鉴定技术的不断提高,越来越多的入侵克氏原杆菌被发现。全世界都有关于金氏感染的报道,这表明该生物体是儿童和成人患者心脏和骨骼感染的重要原因。尽管出现了关于K.kingae感染的临床和诊断方面的信息,但该病原体的毒力机制在很大程度上仍然未知。我们的结果表明K. Kingae产生与细胞膜相关的强效蛋白质型白细胞毒素(Ktx)。这是K的第一个证据。Kingae白细胞毒素可能是逃避宿主免疫反应的重要毒力因子。本研究的目的是识别和表征Ktx。在拟议的研究中,我们将:-研究Ktx在不同K。Kingae临床分离株; -序列K。kingae基因组; kingae,然后进行蛋白质鉴定; -鉴定ktx基因并使用随机和定点诱变方法产生ktx突变体; -表征Ktx对不同人类细胞的毒性。鉴定K. Kingae毒力因子对于了解病原体如何引起疾病至关重要。本研究结果对克螟的防治具有重要意义。金戈感染。 公共卫生相关性:Kingella kingae是一种人类口腔细菌,可导致心脏和骨骼系统感染。我们建议刻画K.一种能破坏人类白色血细胞的Kingae毒素。本研究结果对克螟的防治具有重要意义。金戈感染。

项目成果

期刊论文数量(2)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Genome sequence of Kingella kingae septic arthritis isolate PYKK081.
Kingella kingae 化脓性关节炎分离株 PYKK081 的基因组序列。
  • DOI:
    10.1128/jb.00421-12
  • 发表时间:
    2012
  • 期刊:
  • 影响因子:
    3.2
  • 作者:
    Kaplan,JeffreyB;Lo,Chienchi;Xie,Gary;Johnson,ShannonL;Chain,PatrickSG;Donnelly,Robert;Kachlany,ScottC;Balashova,NataliyaV
  • 通讯作者:
    Balashova,NataliyaV
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Nataliya V Balashova其他文献

Nataliya V Balashova的其他文献

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{{ truncateString('Nataliya V Balashova', 18)}}的其他基金

Characterization of a Kingella kingae leukotoxin
Kingella kingae 白细胞毒素的表征
  • 批准号:
    7739090
  • 财政年份:
    2009
  • 资助金额:
    $ 7.8万
  • 项目类别:
ROLE OF CU, ZN SOD IN A. ACTINOMYCETEMCOMITANS
铜、锌、SOD 在伴放线菌中的作用
  • 批准号:
    7230520
  • 财政年份:
    2006
  • 资助金额:
    $ 7.8万
  • 项目类别:
ROLE OF CU, ZN SOD IN A. ACTINOMYCETEMCOMITANS
铜、锌、SOD 在伴放线菌中的作用
  • 批准号:
    7413259
  • 财政年份:
    2006
  • 资助金额:
    $ 7.8万
  • 项目类别:
ROLE OF CU, ZN SOD IN A. ACTINOMYCETEMCOMITANS
铜、锌、SOD 在伴放线菌中的作用
  • 批准号:
    7155129
  • 财政年份:
    2006
  • 资助金额:
    $ 7.8万
  • 项目类别:

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