APPLIED BIOSYSTEMS-3730 DNA ANALYZER (48 capillary)

APPLIED BIOSYSTEMS-3730 DNA 分析仪（48 个毛细管）

• 批准号: 7221640
• 负责人: Benoit de Crombrugghe
• 金额: $ 27.4万
• 依托单位: UNIVERSITY OF TX MD ANDERSON CAN CTR
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7221640
• 关键词: Arts; Back; Blood capillaries; Cancer Center Support Grant; Cancer Patient; Cells; Core Facility; Cost Savings; DNA; DNA Resequencing; DNA Sequence; DNA analysis; Doctor of Medicine; Funding; Genetic; Genetic Polymorphism; Genomics; Genotype; Goals; Human; Institution; Laboratory Research; Medicine; Mus; Nature; Peer Review Grants; Principal Investigator; Process; Publications; Reagent; Reporting; Research; Research Personnel; Running; SNP genotyping; Sampling; Services; Technology; Time; United States National Institutes of Health; University of Texas M D Anderson Cancer Center; Variant; anticancer research; capillary; cost; experience; instrument; member

DESCRIPTION (provided by applicant): The DNA Analysis Core Facility (DAF) at the University of Texas M. D. Anderson Cancer Center requests funds for the purchase of a 3730 DNA Genetic Analyzer (48capillary instrument) to perform DNA sequencing, resequencing, fluorescent fragment analysis and SNPlex analysis (for identifying polymorphisms and sequence variants). The DAF is a CCSG supported centralized Core facility that provides services to 187 of principal investigators in 44 departments at M.D. Anderson; 74% hold peer-reviewed grants. When NIH investigators are not using these services, they are made available to other members of the institution. The DAF which opened in 1992 has become an integral part of the research laboratories at M.D. Anderson. Members of the institution have come to rely heavily on the facility to provide fast and accurate DNA services, allowing researchers to progress more rapidly with their research goals. The facility is currently supervised by Ms. Erika Thompson M.S. Ms. Thompson has 11 years experience in DNA analysis and has been managing the facility since 2003. The requested instrument will: 1) increase sequencing throughput, processing 48 samples in 2.5hour for DNA sequencing, versus only 16 samples on the 3100 instrument which the 3730 Analyzer will replace, 2) provide significant cost savings e.g. running costs on 3100, $0.60 per sample versus $0.10 on 3730, 3) permit the use of the latest SNPlex technology to rapidly identify up to 48 SNP genotypes per sample in one run. This is ability to multiplex 48 SNPs per sample is especially important in the analysis of small amounts of DNA from cancer patients as SNPlex requires less than 0.8ng of genomic DNA per genotype. The 3730 genetic analyzer will allow the facility to meet the growing demands for existing services and also allow the latest technology in the form of VariantSEQr and SNPlex to be made available to NIH supported investigators at M.D Anderson. At this time the facility cannot provide these additional services as the 3730 XL Genetic analyzer is being used solely for DNA sequencing and the remaining 3100 for genotyping human and mouse samples using fluorescent fragment analysis technology. The DAF has supported the completion of several hundred publications including studies reported in Nature Medicine, Nature Genetics, and Cell. The purchase of a 3730, would allow the facility to increase throughput, reduce turnaround time, provide PIs access to the latest technology (SNPlex and VariantSEQr) and reduce reagent cost. This instrument would also serve as a back up to the 3730XL instrument (the 96 capillary instrument). The 3730 XL, which was purchased by M.D. Anderson in 2002, is currently the workhorse of the facility, and is now reaching capacity. It is critical that the DAF obtain a state of the art, 3730 Genetic Analyzer, so that the core can continue to provide NIH funded investigators with the latest technology to support cancer research.

描述（由申请人提供）：得克萨斯大学M。D.安德森癌症中心申请资金购买3730型DNA遗传分析仪（48毛细管仪器），用于进行DNA测序、重测序、荧光片段分析和SNPs分析（用于识别多态性和序列变异）。该中心是CCSG支持的集中核心设施，为MD 44个部门的187名主要研究者提供服务。安德森; 74%持有同行评审的赠款。当NIH研究人员不使用这些服务时，他们可以提供给机构的其他成员。该实验室于1992年开放，现已成为医学博士研究实验室的一个组成部分。安德森。该机构的成员已经严重依赖该设施提供快速准确的DNA服务，使研究人员能够更快地实现他们的研究目标。该设施目前由Erika Thompson女士监督。汤普森女士拥有11年的DNA分析经验，自2003年以来一直管理该设施。所要求的文书将：1）增加测序通量，在2.5小时内处理48个样本进行DNA测序，而3730分析仪将取代的3100仪器上仅处理16个样本，2）提供显著的成本节约，例如，3100上的运行成本为每个样本0.60美元，而3730上的运行成本为0.10美元，3）允许使用最新的SNP技术在一次运行中快速鉴定每个样品多达48个SNP基因型。这是多重48个SNP/样品的能力在分析来自癌症患者的少量DNA中特别重要，因为SNP/基因型需要少于0.8ng的基因组DNA。3730遗传分析仪将使该设施能够满足对现有服务日益增长的需求，并使最新技术以VariantSEQ r和SNESTs的形式提供给NIH支持的M.D安德森研究人员。目前，该机构无法提供这些额外服务，因为3730 XL遗传分析仪仅用于DNA测序，其余3100台使用荧光片段分析技术对人类和小鼠样本进行基因分型。该基金会支持完成了数百篇出版物，包括在《自然医学》、《自然遗传学》和《细胞》上报道的研究。购买3730将使该机构能够提高吞吐量，缩短周转时间，为PI提供最新技术（SNARY和VariantSEQ r）并降低试剂成本。该仪器也可用作3730 XL仪器（96毛细管仪器）的备份。3730 XL由M.D.安德森在2002年，目前是该设施的主力，现在正在达到能力。至关重要的是，该中心获得最先进的3730遗传分析仪，以便该中心能够继续为NIH资助的研究人员提供最新技术，以支持癌症研究。