DNA Analysis Facility

DNA分析设备

• 批准号: 7695929
• 负责人: Benoit de Crombrugghe
• 金额: $ 28.48万
• 依托单位: UNIVERSITY OF TX MD ANDERSON CAN CTR
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-28 至 2013-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7695929
• 关键词: Arts; Basic Science; Behavioral Sciences; Biological Assay; Biological Process; Biology; Bone Marrow Transplantation; Cancer Biology; Cancer Center; Cancer Center Support Grant; Cancer Immunology Science; Capillary Electrophoresis; Chemistry; Clinical; Committee Members; Communities; Complementary DNA; Computer software; Consultations; Core Facility; Custom; DNA; DNA Resequencing; DNA Sequence; DNA Sequencing Facility; DNA analysis; Daily; Data; Data Analyses; Detection; Development; Disease; Doctor of Medicine; Dyes; Epidemiology; Equipment; Feedback; Fees; Floor; Funding; Gene Expression; Gene Targeting; Genes; Genetic; Genomics; Genomics Shared Resource; Genotype; Goals; Grant; Growth; Head and Neck Cancer; High Pressure Liquid Chromatography; Hospitals; Housing; Individual; Institution; Label; Laboratories; Ligation; Loss of Heterozygosity; Maintenance; Malignant Neoplasms; Malignant neoplasm of brain; Malignant neoplasm of lung; Methods; Microsatellite Instability; Microsatellite Repeats; Molecular; Molecular Genetics; Monitor; Mus; Mutation Detection; Neoplasm Metastasis; Nucleic Acids; Numbers; Oligonucleotides; Operative Surgical Procedures; Output; Pattern; Peer Review; Performance; Plasmids; Polymerase Chain Reaction; Process; Protocols documentation; Quality Control; RNA; Radiation Oncology; Reaction; Reagent; Reporting; Research; Research Personnel; Resource Sharing; Reverse Transcription; Robotics; Room, Clean; Running; SNP genotyping; Sampling; Services; Standards of Weights and Measures; Support of Research; System; Tail; Technical Expertise; Techniques; Technology; Text; Time; Tissues; Today; Variant; Work; base; cancer immunology function; cancer pharmacology; cancer prevention; carcinogenesis; cost; day; density; design; drug development; ds-DNA; falls; gene therapy; instrument; instrumentation; malignant breast neoplasm; member; programs; research study; size

Since its inception in 1991, the goal of the DNA Analysis Facility (DAF) has been to provide MDACC investigators with reliable nucleic acid analysis using state-of-the-art technology to provide a high level of technical expertise in a centralized facility. Today, 7 highly-skilled technical staff operate 3 robotic workstations: a QiagenSOOO Biorobot, a Qiagen 8000 Biorobot, and a Beckman Biomek 2000. The staff also operate a Transgenomic WAVE mutation detection system, an Applied Biosystems 3730 XL genetic analyzer, an Applied Biosystems 3130 genetic analyzer, an Applied Biosystems 3100 genetic analyzer and 4 Applied Biosystems real-time PCR instruments; the 7000, 7300, 7500 and 7900 with low density array. The DAF performs automated DNA sequencing, gene resequencing, fluorescent fragment analysis, highthroughput SNP analysis, murine DNA extraction and genotyping by real-time PCR, quantitative gene expression analysis and real-time PCR assay design. Provision of these essential services has freed investigators to focus their efforts on characterizing the molecular and biological functions of their target genes. A shared resource director, co-director, and a 5-member oversight committee direct the development of the DAF. This committee meets annually to evaluate the scientific and financial performance of the facility. The co-director manages the daily activities of the DAF. In the current grant period, the institution invested $569,500 in the DAF for the purchase of new equipment, in addition to providing a new 1,600 sq ft lab at a cost of $876,000. Funding for the DAF is provided by the CCSG (38%) and user fees (62%). The DAF successfully processed 560,413 samples in the last 5 years, representing 280 investigators from 20 programs. The total number of samples processed increased 198% compared to the previous grant period. All services had more than 75% use by peer-reviewed investigators, with more than 90% of samples processed for peer-reviewed investigators. Output in DNA sequencing during the present grant period compared to the last showed an increase of 130%, while Fragment analysis increased 158%. Newer services have also shown significant growth. Murine genotyping increased 103%, gene resequencing increased 718% (2 years) and 14,600 SNPs were determined using the SNPlex service. The DAF recently introduced a quantitative gene expression analysis service. This service includes RNA quantification, RNA QC, reverse transcription and assay set-up. The facility plans to expand this service to include microRNA assays.

自1991年成立以来，DNA分析设施（DAF）的目标一直是提供MDACC 研究人员使用最先进的技术进行可靠的核酸分析，以提供高水平的 集中设施的技术专长。今天，7名高技能的技术人员操作3台机器人 工作站：QiagenSOOO Biorobot、Qiagen 8000 Biorobot和Beckman Biomek 2000。食堂工作人员也 操作转基因WAVE突变检测系统，Applied Biosystems 3730 XL遗传分析仪， 分析仪、Applied Biosystems 3130遗传分析仪、Applied Biosystems 3100遗传分析仪和4台 Applied Biosystems实时PCR仪器; 7000、7300、7500和7900低密度阵列。的 测序仪进行自动DNA测序、基因重测序、荧光片段分析、高通量 SNP分析，小鼠DNA提取和实时PCR基因分型，定量基因 表达分析和实时PCR测定设计。这些基本服务的提供， 研究人员集中精力描述其目标的分子和生物学功能 基因.一个共享资源主管、联合主管和一个5人监督委员会指导开发 的。该委员会每年举行一次会议，以评估该设施的科学和财务业绩。 联合主任负责管理联合调查组的日常活动。在当前的授予期内，该机构投资了 569，500元的额外拨款，除了在一个位于 费用876000元。该计划的资金由CCSG（38%）和用户费（62%）提供。的DAF 在过去5年中，成功处理了560，413个样本，代表了来自20个国家的280名调查员。 程序.与上一个赠款期相比，处理的样本总数增加了198%。 所有服务都有超过75%的使用同行评审的调查人员，超过90%的样本 为同行评审的研究者处理。本赠款期内DNA测序的产出 与上一次相比显示增加了130%，而片段分析增加了158%。较新 服务业也有显著增长。小鼠基因分型增加103%，基因重测序 增加了718%（2年）和14，600 SNPs使用SNPs服务确定。最近， 推出了定量基因表达分析服务。这项服务包括RNA定量，RNA QC、逆转录和试验设置。该设施计划将这项服务扩展到包括microRNA 分析。