DNA Analysis Facility

自1991年成立以来，DNA分析设施(DAF)的目标一直是提供MDAcc 调查人员使用最先进的技术进行可靠的核酸分析，提供高水平的 集中设施中的技术专长。今天，7名高技能的技术人员操作着3台机器人 工作站：一台QiagenSOOO Biorobot、一台Qiagen 8000 Biorobot和一台Beckman Biomek 2000。工作人员还 操作转基因波突变检测系统，应用BiosSystems 3730 XL基因 应用生物系统3130遗传分析仪、应用生物系统3100遗传分析仪和4 应用BiosSystems实时PCR仪；7000、7300、7500和7900低密度阵列。这个 DAF执行自动DNA测序、基因重新测序、荧光片段分析、高通量 SNP分析、小鼠DNA提取及实时定量聚合酶链式反应基因分型 表达分析和实时荧光定量聚合酶链式反应的设计。这些基本服务的提供 研究人员将重点放在表征他们目标的分子和生物功能上 基因。一名共享资源主任、联席主任和一个五人监督委员会指导开发工作 是DAF的成员。该委员会每年举行一次会议，评估该设施的科学和财务绩效。 联席董事负责管理数码港发展框架的日常活动。在本授权期内，该机构投资了 569,500美元，用于购买新设备，此外还提供一个占地1,600平方英尺的新实验室 成本为876,000美元。发展援助框架的资金由CCSG(38%)和用户费用(62%)提供。DAF 在过去5年中，成功处理了560,413个样本，代表了来自20个国家的280名调查人员 程序。与上一批赠款相比，处理的样本总数增加了198%。 所有服务都有超过75%的被同行评议的调查人员使用，超过90%的样本 为同行评议的调查人员处理。本赠款期间DNA测序的产出 与上次相比，片段分析增加了130%，而片段分析增加了158%。更新的 服务业也出现了显著增长。小鼠基因分型增加103%，基因重新测序 使用SNPlex服务确定了718%(2年)和14,600个SNP。最近的DAF 推出了定量基因表达分析服务。这项服务包括RNA量化， QC、逆转录和检测装置。该机构计划将这项服务扩展到包括microRNA 化验。

Since its inception in 1991, the goal of the DNA Analysis Facility (DAF) has been to provide MDACC investigators with reliable nucleic acid analysis using state-of-the-art technology to provide a high level of technical expertise in a centralized facility. Today, 7 highly-skilled technical staff operate 3 robotic workstations: a QiagenSOOO Biorobot, a Qiagen 8000 Biorobot, and a Beckman Biomek 2000. The staff also operate a Transgenomic WAVE mutation detection system, an Applied Biosystems 3730 XL genetic analyzer, an Applied Biosystems 3130 genetic analyzer, an Applied Biosystems 3100 genetic analyzer and 4 Applied Biosystems real-time PCR instruments; the 7000, 7300, 7500 and 7900 with low density array. The DAF performs automated DNA sequencing, gene resequencing, fluorescent fragment analysis, highthroughput SNP analysis, murine DNA extraction and genotyping by real-time PCR, quantitative gene expression analysis and real-time PCR assay design. Provision of these essential services has freed investigators to focus their efforts on characterizing the molecular and biological functions of their target genes. A shared resource director, co-director, and a 5-member oversight committee direct the development of the DAF. This committee meets annually to evaluate the scientific and financial performance of the facility. The co-director manages the daily activities of the DAF. In the current grant period, the institution invested $569,500 in the DAF for the purchase of new equipment, in addition to providing a new 1,600 sq ft lab at a cost of $876,000. Funding for the DAF is provided by the CCSG (38%) and user fees (62%). The DAF successfully processed 560,413 samples in the last 5 years, representing 280 investigators from 20 programs. The total number of samples processed increased 198% compared to the previous grant period. All services had more than 75% use by peer-reviewed investigators, with more than 90% of samples processed for peer-reviewed investigators. Output in DNA sequencing during the present grant period compared to the last showed an increase of 130%, while Fragment analysis increased 158%. Newer services have also shown significant growth. Murine genotyping increased 103%, gene resequencing increased 718% (2 years) and 14,600 SNPs were determined using the SNPlex service. The DAF recently introduced a quantitative gene expression analysis service. This service includes RNA quantification, RNA QC, reverse transcription and assay set-up. The facility plans to expand this service to include microRNA assays.