Xenogen IVIS Imaging System 200

描述（由申请人提供）：威斯康星大学麦迪逊分校迫切需要对小动物进行无创实时体内成像。这种需要是围绕着宿主-微生物相互作用的研究。执行这些研究所需的设备是Xenogen IVIS成像系统200。这种实时体内成像的最大好处是使用的小鼠要少得多，因为该仪器允许科学家在整个实验过程中跟踪同一只小鼠，而不是在不同的时间点使用和牺牲几只小鼠。当D-荧光素或腔肠素被注射并分别被萤火虫或海肾藻毒素酶用作底物时，光从活体动物内发射。光谱成像使用来自在420 nm至720 nm范围内的不同波长下过滤的图像序列的测量数据，以确定生物发光报告物的深度和位置。IVIS成像系统200包括灵敏的CCD照相机、使入射光最小化的暗成像室以及允许生物发光和荧光成像的22滤光轮。转塔安装的定制透镜提供5个级别的放大率，从26厘米到2.9厘米，允许高分辨率（60微米）的详细成像。Living Image专业软件分析和量化结果，甚至可以执行3D扩散断层扫描重建以可视化内部器官。所有这些功能-荧光和生物发光成像与多个报告，高分辨率放大，和3D重建-将给我们很大的灵活性与我们的实验。在这组用户中共享这个特定的工具是充分利用技术灵活性的一个很好的例子。该仪器的成像能力将使用户能够可视化小鼠/微生物的相互作用，以及1）夏威夷鱿鱼Euprymna spectroopes及其发光细菌伙伴Vibrio fischeri，2）舞毒蛾中肠的正常微生物植物群和3）线虫之间的三方相互作用之间的模型共生相互作用。（Steinernema carpocapsae）、幼虫期昆虫和γ-变形杆菌Xellobdus cycloophila。威斯康星大学麦迪逊分校以领导宿主-微生物相互作用领域而闻名;我们打算为我们的研究开发这些体内成像模型和试剂，并将其提供给全国各地的其他研究人员。

DESCRIPTION (provided by applicant): There is a dramatic need for noninvasive real-time in vivo imaging of small animals at the University of Wisconsin-Madison. This need is centered around the study of host- microbe interactions. The equipment required to perform these investigations is the Xenogen IVIS Imaging System 200. The most significant benefit of this real-time in vivo imaging is far fewer mice will be used because the instrument allows a scientist to follow the same mouse throughout an experiment instead of using and sacrificing several mice at various time points. Light is emitted from within a living animal when D-Luciferin or Coelenterazine is injected and used as a substrate by firefly or renilla luciferases, respectively. Spectral imaging uses measurement data from a sequence of images filtered at different wavelengths ranging from 420 nm to 720 nm, to determine the depth and location of the bioluminescent reporter. The IVIS Imaging System 200 includes a sensitive CCD camera, a dark imaging chamber to minimize incident light, and a 22-filter wheel that permits both bioluminescent and fluorescent imaging. Turret-mounted custom lens provide 5 levels of magnification, from 26 cm to 2.9 cm, allowing high-resolution (60 ¿m) detailed imaging. Living Image specialized software analyzes and quantifies the results, and can even perform 3D diffuse tomography reconstructions to visualize internal organs. All of these features - fluorescent and bioluminescent imaging with multiple reporters, high-resolution magnification, and 3D reconstruction - will give us great flexibility with our experiments. Sharing this particular instrument among this group of users is a beautiful example of exploiting the flexibility of a technology to its fullest. This instrument's imaging capabilities will enable users to visualize mouse/microbe interactions as well as the model symbiotic interactions between 1) the Hawaiian squid Euprymna scolopes and its luminous bacterial partner Vibrio fischeri, 2) the normal microbial flora of the gypsy moth midgut, and 3) the tripartite interaction between a nematode (Steinernema carpocapsae), larval stage insects, and a gamma-proteobacterium, Xenorhabdus nematophila. The University of Wisconsin-Madison is well known for leading the field of host-microbe interactions; it is our intent to develop these in vivo imaging models and reagents for our studies, and to make them available to other investigators across the country.