BD FACSAria Flow Cytometer

BD FACSAria 流式细胞仪

• 批准号: 7218353
• 负责人: Laurent Brossay
• 金额: $ 40.3万
• 依托单位: BROWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-22 至 2008-03-21
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7218353
• 关键词: Arts; Biology; Cell Separation; Cells; Color; Communities; Development; Direct Costs; Flow Cytometry; Funding; Grant; Hospitals; Immune response; Malignant Neoplasms; Pharmaceutical Preparations; Pliability; Postdoctoral Fellow; Purpose; Range; Research; Research Personnel; Research Project Grants; Rhode Island; Running; Speed; Sterility; Students; United States National Institutes of Health; Universities; instrument; pathogen

DESCRIPTION (provided by applicant): The purpose of this application is to request funding for a FACSAria cell sorter at Brown University. This FACSAria has been chosen because it is a versatile instrument that can do both cell analysis and cell sorting. This is critical for the Brown scientific community because there is only one outdated and limited cell sorter available and no machine that can analyze more than four colors simultaneously. Over 40 post-doctoral fellows, graduate students, and technicians perform studies that are dependent on the current FACSCalibur and autoMACS for analysis, isolation of cells and cell sorting. The new sorter will be configured for maximal spectral range, flexibility and speed of cell sorting, and sterility of sorted cells. This instrument will greatly benefit the Rhode Island scientific community because i) sophisticated cell sorting and analysis is critical for modern biology; ii) the current cell sorter is not reliable and the flow cytometry instruments are already running beyond their capacity; and iii) the current flow cytometry facility limits the progress of many NIH funded research projects at Brown University. A group of ten major users within Brown University and affiliated hospitals has been identified. These ten major users project 70% of the total use of the FACSAria cytometer and hold, as PI, NIH grants with around $5,000,000 in annual direct costs. In addition, there are over 100 PIs in the community who could benefit from this instrument as non-main users. The acquisition of a state of the art cell sorter is critical for many Rhode Island investigators who study cancer or immune response to pathogens. It will impact their research immediately and help to promote the development of new drugs.

描述（由申请人提供）：本申请的目的是申请资助布朗大学的FACSAria细胞分选仪。之所以选择FACSAria，是因为它是一种多功能仪器，可以进行细胞分析和细胞分选。这对布朗科学界来说至关重要，因为只有一台过时且有限的细胞分选机可用，而且没有一台机器可以同时分析四种以上的颜色。超过40名博士后研究员，研究生和技术人员进行研究，这些研究依赖于当前的FACSCalibur和autoMACS进行分析，细胞分离和细胞分选。新的分选机将被配置为最大的光谱范围，灵活性和细胞分选的速度，以及分选细胞的无菌性。该仪器将极大地有利于罗得岛科学界，因为i）复杂的细胞分选和分析对于现代生物学至关重要; ii）当前的细胞分选仪不可靠，并且流式细胞仪仪器已经超出其能力运行;以及iii）当前的流式细胞仪设施限制了布朗大学的许多NIH资助的研究项目的进展。已经确定了布朗大学及其附属医院的十个主要用户。这10个主要用户预计占FACSAria细胞仪总使用量的70%，并作为PI持有NIH赠款，每年直接费用约为5，000，000美元。此外，社会上有超过100名专业督察可以作为非主要用户从这一工具中受益。对于研究癌症或对病原体的免疫反应的许多罗得岛研究人员来说，获得最先进的细胞分选仪至关重要。这将立即影响他们的研究，并有助于促进新药的开发。

项目成果

Decreased lung carcinoma cell functions on select polymer nanometer surface features.

• DOI: 10.1002/jbm.a.33217
• 发表时间: 2012
• 期刊: Journal of biomedical materials research. Part A
• 作者: Lijuan Zhang;T. Webster

Measuring the activity of BioBrick promoters using an in vivo reference standard.

• DOI: 10.1186/1754-1611-3-4
• 发表时间: 2009-03-20
• 期刊: Journal of biological engineering
• 影响因子: 5.6
• 作者: Kelly JR;Rubin AJ;Davis JH;Ajo-Franklin CM;Cumbers J;Czar MJ;de Mora K;Glieberman AL;Monie DD;Endy D
• 通讯作者: Endy D

Poly-lactic-glycolic-acid surface nanotopographies selectively decrease breast adenocarcinoma cell functions.

聚乳酸乙醇酸表面纳米拓扑结构选择性地降低乳腺腺癌细胞功能。

• DOI: 10.1088/0957-4484/23/15/155101
• 发表时间: 2012
• 期刊: Nanotechnology
• 影响因子: 3.5
• 作者: Zhang,Lijuan;Webster,ThomasJ
• 通讯作者: Webster,ThomasJ

Cross-activating invariant NKT cells and kupffer cells suppress cholestatic liver injury in a mouse model of biliary obstruction.

跨激活不变的NKT细胞和库普弗细胞在胆道阻塞的小鼠模型中抑制胆汁淤积性肝损伤。

• DOI: 10.1371/journal.pone.0079702
• 发表时间: 2013
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Duwaerts CC;Sun EP;Cheng CW;van Rooijen N;Gregory SH
• 通讯作者: Gregory SH

Contrasting responses of Kupffer cells and inflammatory mononuclear phagocytes to biliary obstruction in a mouse model of cholestatic liver injury.

• DOI: 10.1111/liv.12048
• 发表时间: 2013-02
• 期刊: Liver international : official journal of the International Association for the Study of the Liver
• 作者: Duwaerts CC;Gehring S;Cheng CW;van Rooijen N;Gregory SH
• 通讯作者: Gregory SH