Electrochemistry & Spectroscopy of Novel Protein/Nanomaterial-Based Bioelectrodes

电化学

• 批准号: 7596691
• 负责人: Hiroyasu Tachikawa
• 金额: $ 14.16万
• 依托单位: JACKSON STATE UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7596691
• 关键词: Atomic Force Microscopy; Behavior; Biochemistry; Biomedical Research; Biosensor; Calcium; Carboxy-Lyases; Cytochromes; Detection; Development; Dioxygen; Electrochemistry; Electrodes; Electron Transport; Enzymes; Fast Electron; Glucose; Grant; Histamine; Immobilization; Immobilized Enzymes; Lasers; Measures; Medical center; Metals; Methods; Mississippi; Myoglobin; Nanotubes; Nitric Oxide; Physiologic pulse; Polymers; Protein Chemistry; Proteins; Pulse taking; Rate; Silicon Dioxide; Solutions; Spectrum Analysis; Sulfhydryl Compounds; Surface; System; TNFRSF5 gene; Transducers; Universities; base; cofactor; monolayer; nanomaterials; nanoparticle; novel; professor; sensor; surfactant

Conducting polymers have been used to modify electrode surfaces and to immobilize enzymes to construct biosensors for the detection of biomedically important species such as nitric oxide(NO), glucose, histamine, etc. We will use other substrates such as nanomaterials including nanotubes and nanoparticles, silica solgel, surfactants, and self-assembled monolayers (SAMs) to modify electrode surfaces for the immobilization of biomolecules in order to develop sensors for biomedically important species such as NO, thiols, histamine, and calcium. During the current grant period we have successfully measured the formal potentials of myoglobin and cytochromes by direct electrochemistry at electrodes modified with those substrates. We will use this approach to study several novel proteins such as pirin, decarboxylase enzymes, and diheme proteins by collaborating with two biochemistry professors at the University of Mississippi Medical Center. The hypotheses for the study are as follows:(1) the electrodes modified with those substrates help to carry out direct electrochemistry of novel proteins, and (2) the novel proteins on (or in) those substrates maintain the native form or at least these proteins are not denatured. Electrochemical methods such as cyclic voltammetry (CV), differential pulse voltammetry (DPV), and square wave voltammetry (SWV) will be used to determine the formal potentials of those proteins on (or in) different substrates. Electrochemistry will also be used to probe the interaction of the metal center (e.g., Fe) with dioxygen, NO, and the related transcriptional cofactors such as P50. These studies will help to find protein/matrix systems which may provide a fast electron transfer rate between the protein molecule and the substrate for the development of more sensitive transducers. These electrochemical studies will also provide valuable information for the protein chemistry involved in biomedical research. Those protein molecules on the modified electrodes as well as in solutions will also be characterized by spectroscopic methods including laser-Raman, FT-IR, UV-vis, atomic force microscopy (AFM), TEM, and SEM. These studies will provide spectroscopic evidence for the interactions between enzyme proteins and the modified electrodes and may be able to explain the differences.in electrochemical behaviors of protein molecules on different substrates.

导电聚合物已用于修饰电极表面并固定酶以构建 用于检测生物医学重要物质的生物传感器，例如一氧化氮 (NO)、葡萄糖、组胺、 等。我们将使用其他基材，例如纳米材料，包括纳米管和纳米颗粒，二氧化硅溶胶凝胶， 表面活性剂和自组装单层 (SAM) 来修饰电极表面以进行固定 生物分子，以开发生物医学重要物种的传感器，例如一氧化氮、硫醇、组胺、 和钙。在当前的资助期内，我们已经成功地衡量了以下项目的正式潜力： 在用这些底物修饰的电极上通过直接电化学来检测肌红蛋白和细胞色素。我们将 使用这种方法研究几种新型蛋白质，例如吡林、脱羧酶和二血红素 与密西西比大学医学中心的两位生物化学教授合作研究蛋白质。 研究假设如下：（1）用这些底物修饰的电极有助于携带 新型蛋白质的直接电化学，以及（2）这些底物上（或内）的新型蛋白质维持 天然形式或至少这些蛋白质没有变性。电化学方法如循环法 伏安法（CV）、微分脉冲伏安法（DPV）和方波伏安法（SWV）将用于 确定这些蛋白质在不同基质上（或其中）的形式势。电化学也将 用于探测金属中心（例如 Fe）与双氧、NO 的相互作用以及相关的转录 辅因子如P50。这些研究将有助于找到可以提供快速 蛋白质分子和底物之间的电子转移速率，用于开发更灵敏的 换能器。这些电化学研究也将为蛋白质化学提供有价值的信息 参与生物医学研究。修饰电极上以及溶液中的那些蛋白质分子 还将通过光谱方法进行表征，包括激光拉曼、FT-IR、UV-vis、原子力 显微镜 (AFM)、TEM 和 SEM。这些研究将为相互作用提供光谱证据 酶蛋白和修饰电极之间的差异也许能够解释这些差异。 蛋白质分子在不同基质上的电化学行为。