RNASE P

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心机构，不一定是研究者机构。酵母核RNase P负责前体tRNA的5'末端的成熟。有几条证据使我们相信RNase P有额外的底物，我们最近已经确定了几个候选者。目前正在努力确定核糖核酸酶P已知核心之外的相互作用伙伴。使用新的亲和标签，我们能够快速分离全酶，并期望找到相互作用的蛋白质，这些蛋白质在最初的艰难纯化过程中丢失，导致RNase P核心的鉴定。这些相互作用的合作伙伴将调查他们的作用，在处理新的基板候选人最近在我们的实验室。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The yeast nuclear RNase P is responsible for the maturation of the 5' end of precursor tRNAs. There are several lines of evidence that lead us to believe that RNase P has additional substrates and we have recently identified several candidiates. Work to attempt the identification of interacting partners outside the known core of RNase P is being carried out. Using novel affinity tags we are able to rapidly isolate the holoenzyme and expect to find interacting proteins that were lost during the initiual arduous purification that led to the identification of the RNase P core. These interacting partners will be investigated for their roles in the processing of novel substrate candidates recently identified in our lab.