RNASE P

核糖核酸酶P

• 批准号: 8171431
• 负责人: DAVID R ENGELKE
• 金额: $ 0.24万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171431
• 关键词: Affinity; Computer Retrieval of Information on Scientific Projects Database; Funding; Grant; Holoenzymes; Institution; Lead; Nuclear; Process; Proteins; RNase P; Research; Research Personnel; Resources; Ribonucleases; Role; Source; United States National Institutes of Health; Work; Yeasts; novel

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The yeast nuclear RNase P is responsible for the maturation of the 5' end of precursor tRNAs. There are several lines of evidence that lead us to believe that RNase P has additional substrates and we have recently identified several candidiates. Work to attempt the identification of interacting partners outside the known core of RNase P is being carried out. Using novel affinity tags, we are able to rapidly isolate the holoenzyme and expect to find interacting proteins that were lost during the initial arduous purification that led to the identification of the RNase P core. These interacting partners will be investigated for their roles in the processing of novel substrate candidates recently identified in our lab.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 酵母核RNase P负责前体tRNA的5'末端的成熟。有几条证据使我们相信RNase P有额外的底物，我们最近已经确定了几个候选者。 目前正在努力确定核糖核酸酶P已知核心之外的相互作用伙伴。使用新型亲和标签，我们能够快速分离全酶，并期望找到在最初的艰难纯化过程中丢失的相互作用蛋白，从而鉴定RNase P核心。这些相互作用的合作伙伴将调查他们的作用，在处理新的基板候选人最近在我们的实验室。