MULTISCALE MODELS FOR PACKAGING VIRAL RNAS

用于包装病毒 RNA 的多尺度模型

• 批准号: 7358855
• 负责人: STEPHEN C. HARVEY
• 金额: $ 5.52万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-01 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7358855
• 关键词: MULTISCALE; MODELS; PACKAGING; VIRAL; RNAS

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The crystal structure of pariacoto virus (PaV) reveals about 35% of the RNA genome. This RNA forms a dodecahedral cage just under the capsid (Tang et al., 2001). In last year¿s progress report, we reported development a model for the phosphate positions of the remaining 65% of the RNA. The double-helical regions of the dodecahedral cage in our model are essentially identical to those in the crystal structure. The three-way junctions are disordered in the crystal structure, suggesting structural heterogeneity at the threefold crystallographic axes. Our model includes a mixture of three-way junctions, two-way junctions with a stem-loop on the third arm, and four-way junctions. The latter occur when three double helices meet at the threefold axis and the RNA drops down into the interior of the virus from that axis, forming the fourth arm of the junction.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是中心的机构，不一定是研究者的机构。 pariacoto 病毒 (PaV) 的晶体结构揭示了约 35% 的 RNA 基因组。这种 RNA 在衣壳下方形成十二面体笼 (Tang et al., 2001)。在去年的进展报告中，我们报告了针对剩余 65% RNA 磷酸盐位置的开发模型。我们模型中十二面体笼的双螺旋区域与晶体结构中的双螺旋区域基本相同。三向连接在晶体结构中是无序的，表明三重晶体轴处的结构异质性。我们的模型包括三路连接点、第三臂上有茎环的双向连接点和四路连接点的混合。后者发生在三个双螺旋在三重轴处相遇时，RNA 从该轴落入病毒内部，形成连接点的第四臂。