MULTISCALE MODELS FOR PACKAGING VIRAL RNAS

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。Pariacoto病毒(PAV)的晶体结构揭示了约35%的RNA基因组。该RNA在衣壳下形成一个十二面体笼子(Tang等人，2001年)。在去年的S进度报告中，我们报道了剩余65%的RNA的磷酸位置的开发模型。在我们的模型中，十二面体笼子的双螺旋区域与晶体结构中的区域基本相同。三向结在晶体结构上是无序的，这表明在三重结晶轴上的结构不均匀。我们的模型包括三向连接、在第三臂上带有茎环的双向连接和四向连接的混合。当三个双螺旋在三重轴相遇，RNA从三重轴下降到病毒内部，形成连接的第四个臂时，就会发生后一种情况。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The crystal structure of pariacoto virus (PaV) reveals about 35% of the RNA genome. This RNA forms a dodecahedral cage just under the capsid (Tang et al., 2001). In last year¿s progress report, we reported development a model for the phosphate positions of the remaining 65% of the RNA. The double-helical regions of the dodecahedral cage in our model are essentially identical to those in the crystal structure. The three-way junctions are disordered in the crystal structure, suggesting structural heterogeneity at the threefold crystallographic axes. Our model includes a mixture of three-way junctions, two-way junctions with a stem-loop on the third arm, and four-way junctions. The latter occur when three double helices meet at the threefold axis and the RNA drops down into the interior of the virus from that axis, forming the fourth arm of the junction.