Molecular Biology of Eukaryotic Viruses

描述(由申请人提供)：本提案是一项竞争性的续签申请，旨在继续支持1988年在加州大学欧文分校(UCI)建立的真核病毒分子生物学培训计划。这个项目的目标是在分子病毒学的基本方面培训博士研究生，因为分子病毒学与基因表达调控、病毒结构、病毒-宿主相互作用和致病机制有关。在过去的审查期间(9/02-8/07)，8名博士后研究生获得了该培训计划的支持。建议在未来五年在结构生物学/蛋白质组学、病毒-宿主相互作用和需要BSL-3遏制的病毒领域扩大跨学科研究和培训计划。该项目的教师导师人数已扩大到15人，代表UCI的四个学术部门。这些系的教员与生物科学学院和医学院其他系的教员一起参加UCI分子生物学、遗传学和生物化学研究生项目(MBGB)，该项目负责博士前学生的招生、录取和初步培训。所有NIH病毒学助学金学员都属于MBGB计划中的病毒学专业。这一轨道由在病毒学和相关学科中有共同研究兴趣的教职员工、学生、博士后研究员和实验室工作人员组成。病毒学培训计划包括病毒基因表达、病毒感染的分子发病机制和疾病的免疫致病机制等选修课。教员的研究项目包括病毒基因组复制、病毒特异性转录、RNA加工、病毒翻译和蛋白质加工、病毒结构蛋白的组装和运输以及病毒颗粒的结构。病毒学教授还在研究病毒与宿主的相互作用，包括宿主调控分子的改变、生长控制、细胞周期调控、病毒基因组整合到宿主细胞DNA的机制，以及宿主功能对病毒基因表达的颠覆。正在研究的病毒/病毒系统包括小鼠白血病病毒、绵羊逆转录病毒(JSRV)、艾滋病毒、酵母Ty3、脊髓灰质炎病毒、人鼻病毒、柯萨奇病毒、小鼠肝炎病毒、SARS冠状病毒、ArenaVirus、单纯疱疹病毒、Epstein-Barr病毒、人类乳头瘤病毒、牛痘病毒和一些含有RNA的植物病毒。

DESCRIPTION (provided by applicant): This proposal is a competing renewal application for continuing support of a training program in the Molecular Biology of Eukaryotic Viruses, established at the University of California, Irvine (UCI) in 1988. The goal of this program is to train Ph.D. graduate students in the fundamental aspects of molecular virology as they relate to the regulation of gene expression, virus structure, virus-host interactions, and pathogenesis. During the past review period (9/02-8/07), eight predoctoral students were supported by this training program. An expansion of interdisciplinary research and training programs in the areas of structural biology/proteomics, virus-host interactions, and viruses requiring BSL-3 containment is proposed for the next five years. The number of faculty mentors for this program has been expanded to 15, representing four academic departments at UCI. Faculty members from these departments, along with those from other departments in the School of Biological Sciences and the School of Medicine, participate in the Graduate Program in Molecular Biology, Genetics, & Biochemistry (MBGB) at UCI, which oversees recruitment, admission, and initial training of predoctoral students. All of the NIH Virology Grant trainees belong to the Virology Track in the MBGB program. This track is comprised of faculty, students, postdoctoral fellows, and laboratory staff who have shared research interests in virology and related disciplines. The Virology training program includes elective courses in viral gene expression, molecular pathogenesis of viral infections, and immunopathogenic mechanisms of disease. The research programs of faculty participants include the study of viral genome replication, viral-specific transcription, RNA processing, viral translation and protein processing, assembly and transport of viral structural proteins, and the structures of virus particles. The virology faculty are also studying virus-host interactions that include alteration of host regulatory molecules, growth control, cell cycle regulation, the mechanisms of integration of viral genomes into host cell DNA, and the subversion of host functions for virus gene expression. The viruses/viral systems being studied include murine leukemia virus, a sheep retrovirus (JSRV), HIV, yeast Ty3, poliovirus, human rhinovirus, coxsackievirus, mouse hepatitis virus, SARS coronavirus, arenaviruses, herpes simplex virus, Epstein-Barr virus, human papillomavirus, vaccinia virus, and a number of RNA-containing plant viruses.