Core--Cryopreservation and Embryo Derivation

核心--冷冻保存与胚胎衍生

• 批准号: 7481093
• 负责人: Shizuo Akira
• 金额: $ 31.13万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7481093
• 关键词: Collaborations; Communities; Cryopreservation; Derivation procedure; Embryo; Freezing; Generations; Genetic; Immune response; Induced Mutation; Institutes; Japan; Knock-out; Knockout Mice; Laboratories; Microinjections; Mouse Strains; Mus; Mutant Strains Mice; Mutation; Natural Immunity; Outcome; Purpose; Quarantine; Research; Research Institute; Shipping; Ships; Signal Pathway; Universities; Viral; Viral Pathogenesis; Virus; base; blastocyst; embryonic stem cell; gene interaction; mouse model; mutant; natural Blastocyst Implantation; positional cloning; research study; success

The functions of the Cryopreservation and Embryo Derivation Core are: A) to expeditiously transfer mutant mice created by the Osaka group in Japan to the La Jolla group. B) to rederive frozen embryos shipped from La Jolla to Osaka. C) to generate ES cell-derived chimeric mice for the establishment of knockout mice by microinjection of ES cells into mouse blastocysts and implantation. D) to submit mutant mouse strains to the MMRRC for distribution to the scientific community. Generation of mouse models have made major contributions to recent advances in the field of innate immunity. In the proposed project to investigate anti-viral immune responses by both forward and reverse genetics, close collaboration between the La Jolla group (conducting mouse forward genetics) and the Osaka group (conducting mouse reverse genetics) will be essential for success. We will exchange mutant mice and will study them in parallel using different viruses. We will also analyze signaling pathways based on the outcome of experiments in which ENU-induced mutations and knockout mutations are combined. A rapid and safe means of exchange, circumventing quarantine and allowing quick assessments, will be provided by this Core. The Core will also be responsible for the microinjection of ES cells into mouse blastocysts and re-derivation from the embryos for generating knockout mice. Finally, the Core will also send mutant mouse strains generated in Osaka to the MMRRC for distribution to the scientific community, permitting accelerated research in many laboratories worldwide.

冷冻保存和胚胎衍生核心的功能是：A）快速转移突变体 将日本大坂小组制造的小鼠转移到拉霍亚小组。B）将从洛杉矶运来的冷冻胚胎重新衍生 Jolla到大坂。C）通过以下步骤产生ES细胞衍生的嵌合小鼠用于建立敲除小鼠： 将ES细胞显微注射到小鼠胚泡中并植入。D）将突变小鼠品系提交给 MMRRC分发给科学界。小鼠模型的产生已经使主要的 对先天免疫领域最新进展的贡献。在研究抗病毒药物的拟议项目中， 免疫反应的正向和反向遗传学，密切合作之间的拉霍亚小组 （进行小鼠正向遗传学）和大坂组（进行小鼠反向遗传学）将在 成功的关键。我们将交换突变小鼠，并使用不同的病毒进行平行研究。我们 还将根据ENU诱导突变的实验结果分析信号通路， 和敲除突变的组合。一种快速安全的交换方式，绕过检疫， 允许快速评估，将由该核心提供。核心还将负责 将ES细胞显微注射到小鼠囊胚中并从胚胎中再衍生以产生敲除 小鼠最后，核心还将把在大坂产生的突变小鼠品系送到MMRRC进行分发 科学界，允许在世界各地的许多实验室加速研究。