Core--Cryopreservation and Embryo Derivation

冷冻保存和胚胎衍生核心的功能是：a)快速转移突变体 日本大阪小组创造的小鼠到La Jolla小组。B)重新提取从洛杉矶运来的冷冻胚胎 霍拉去大阪。C)产生ES细胞来源的嵌合体小鼠，用于建立基因敲除小鼠 小鼠囊胚内显微注射ES细胞并植入。D)将突变小鼠品系提交给 MMRRC分发给科学界。一代又一代的老鼠模型已经取得了重大进展 对先天免疫领域最新进展的贡献。在拟议的研究抗病毒的项目中 正向和反向遗传学的免疫反应，La Jolla小组之间的密切合作 (进行小鼠正向遗传学)和大阪小组(进行小鼠反向遗传学)将 对于成功来说是必不可少的。我们将交换突变小鼠，并使用不同的病毒对它们进行平行研究。我们 我还将根据ENU诱导突变的实验结果来分析信号通路 和基因敲除突变结合在一起。一种快速安全的交换手段，绕过检疫和 允许快速评估，将由此核心提供。核心还将负责 将ES细胞显微注射到小鼠囊胚中并从胚胎中重新衍生产生基因敲除 老鼠。最后，Core还将把在大阪产生的突变小鼠品系送到MMRRC进行分发 对科学界来说，允许在世界各地的许多实验室加速研究。

The functions of the Cryopreservation and Embryo Derivation Core are: A) to expeditiously transfer mutant mice created by the Osaka group in Japan to the La Jolla group. B) to rederive frozen embryos shipped from La Jolla to Osaka. C) to generate ES cell-derived chimeric mice for the establishment of knockout mice by microinjection of ES cells into mouse blastocysts and implantation. D) to submit mutant mouse strains to the MMRRC for distribution to the scientific community. Generation of mouse models have made major contributions to recent advances in the field of innate immunity. In the proposed project to investigate anti-viral immune responses by both forward and reverse genetics, close collaboration between the La Jolla group (conducting mouse forward genetics) and the Osaka group (conducting mouse reverse genetics) will be essential for success. We will exchange mutant mice and will study them in parallel using different viruses. We will also analyze signaling pathways based on the outcome of experiments in which ENU-induced mutations and knockout mutations are combined. A rapid and safe means of exchange, circumventing quarantine and allowing quick assessments, will be provided by this Core. The Core will also be responsible for the microinjection of ES cells into mouse blastocysts and re-derivation from the embryos for generating knockout mice. Finally, the Core will also send mutant mouse strains generated in Osaka to the MMRRC for distribution to the scientific community, permitting accelerated research in many laboratories worldwide.