High Frequency Stimulation Control of Axonal Conduction

轴突传导的高频刺激控制

• 批准号: 7498013
• 负责人: ALICIA L JENSEN
• 金额: $ 0.18万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-12-01 至 2008-04-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7498013
• 关键词: Alternative Therapies; Automobile Driving; Axon; Be++ element; Beryllium; Brain; Clinical; Computer Simulation; Condition; Conflict (Psychology); Data; Deep Brain Stimulation; Dendrites; Development; Electric Stimulation; Electrophysiology (science); Epilepsy; Extracellular Space; Frequencies; Generations; Goals; Hippocampus (Brain); Homeostasis; In Vitro; Ions; Lesion; Long-Term Effects; Mediating; Methods; Mind; Molecular; Nail plate; Neuraxis; Neurons; Outcomes Research; Population; Potassium; Potassium Channel; Preparation; Publishing; Pyramidal Cells; Rattus; Research; Role; Seizures; Slice; Structure; Technology; Testing; Therapeutic; Tissues; alveus; extracellular; in vivo; insight; multidisciplinary; nervous system disorder; neural circuit; neuronal cell body; novel; patch clamp; relating to nervous system; research study; tool

High frequency stimulation (HFS) is a promising method for the therapeutic control of epilepsy. Yet, the mechanisms for HFS mediated control of epilepsy are unknown. The ultimate goal of this research is to assist in the development of novel epilepsy therapies by investigating the mechanisms underlying the effect of HFS on neural tissue. Depolarization blockade, mediated through excess potassium in the extracellular space, has been postulated as one potential mechanism in HFS mediated control of epilepsy. Therefore, the overall objective of this proposal is to investigate relationship between HFS induced effects on neural tissue and potassium dynamics using multidisciplinary experimental approaches. Experiments will focus on the hippocampus as it is known for its' multiple, interrelated neural circuits as well as a foci for generation of epileptiform activity. Electrophysiology will be used to investigate the effects of HFS on neural tissue in vitro and in vivo, while patch clamping and ion-selective recording will explore underlying mechanisms for HFS mediated seizure control. A NEURON computer model will be developed to evaluate these mechanisms under conditions that would normally be difficult or impossible to test experimentally.

高频电刺激（HFS）是一种很有前途的方法，用于治疗控制癫痫。然而 HFS介导的癫痫控制机制尚不清楚。这项研究的最终目的是 通过研究潜在的作用机制，协助开发新的癫痫治疗方法。 对神经组织的影响去极化阻滞，通过细胞外过量钾介导 空间，已被假定为HFS介导的癫痫控制中的一种潜在机制。因此 本建议的总体目标是研究HFS诱导的神经组织效应之间的关系 和钾动力学使用多学科的实验方法。实验将集中在 海马体，因为它是众所周知的'多，相互关联的神经回路，以及一个焦点，为产生 癫痫样活动采用电生理学方法研究HFS对离体神经组织的影响 而膜片钳和离子选择性记录将探索HFS的潜在机制 介导的癫痫控制。将开发一个神经元计算机模型来评估这些机制 在通常难以或不可能通过实验测试的条件下。