High Frequency Stimulation Control of Axonal Conduction

轴突传导的高频刺激控制

• 批准号: 7156509
• 负责人: ALICIA L JENSEN
• 金额: $ 3.27万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-12-01 至 2008-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7156509
• 关键词: axon; electrophysiology; epilepsy; extracellular; model; potassium; suppression; tissues

DESCRIPTION (provided by applicant): High frequency stimulation (HFS) is a promising method for the therapeutic control of epilepsy. Yet, the mechanisms for HFS mediated control of epilepsy are unknown. The ultimate goal of this research is to assist in the development of novel epilepsy therapies by investigating the mechanisms underlying the effect of HFS on neural tissue. Depolarization blockade, mediated through excess potassium in the extracellular space, has been postulated as one potential mechanism in HFS mediated control of epilepsy. Therefore, the overall objective of this proposal is to investigate relationship between HFS induced effects on neural tissue and potassium dynamics using multidisciplinary experimental approaches. Experiments will focus on the hippocampus as it is known for its' multiple, interrelated neural circuits as well as a foci for generation of epileptiform activity. Electrophysiology will be used to investigate the effects of HFS on neural tissue in vitro and in vivo, while patch clamping and ion-selective recording will explore underlying mechanisms for HFS mediated seizure control. A NEURON computer model will be developed to evaluate these mechanisms under conditions that would normally be difficult or impossible to test experimentally.

描述(申请人提供)：高频刺激(HFS)是一种很有前途的癫痫治疗控制方法。然而，HFS介导的癫痫控制机制尚不清楚。这项研究的最终目的是通过研究HFS对神经组织影响的潜在机制来帮助开发新的癫痫治疗方法。去极化阻断是HFS介导癫痫控制的一种潜在机制，它是通过细胞外间隙中过量的钾介导的。因此，这项建议的总体目标是通过多学科的实验方法来研究HFS对神经组织的影响与钾动力学之间的关系。实验将集中在海马体上，因为它以其多个相互关联的神经回路以及癫痫样活动的产生中心而闻名。电生理学将被用来研究HFS在体外和体内对神经组织的影响，而膜片钳和离子选择性记录将探索HFS介导的癫痫控制的潜在机制。将开发一个神经元计算机模型，在通常难以或不可能进行实验测试的条件下评估这些机制。