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Interactions Between Agrobacterium and Host Plants

农杆菌与宿主植物之间的相互作用

基本信息

批准号：
7455782
负责人：
Stephen C. Winans
金额：
$ 44.19万
依托单位：
CORNELL UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1989
资助国家：
美国
起止时间：
1989-07-01 至 2011-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7455782
关键词：
Aeromonas Agrobacterium Animals Antibiotics Apoproteins Bacteria Binding Biochemical Biochemical Genetics Bioinformatics Biology Bioluminescence Blur Burkholderia Cell Communication Cells Chemicals Chromosomes Collaborations Communication Complex DNA-Directed RNA Polymerase Development Diffuse Ecology Enterococcus faecalis Erwinia Family Family member Fruit Functional RNA Gene Expression Regulation Gene Targeting Genes Genetic Goals Health Homologous Gene Human In Vitro Laboratories Leadership Life Ligands Mediating Microbe Microbial Biofilms Modeling Molecular Conformation Myxococcus xanthus Numbers Nutrient Pheromone Pheromone Receptors Physiological Plant Tumor-Inducing Plasmids Plants Plasmids Play Population Density Positioning Attribute Production Property Protein Binding Proteins Proteobacteria Proteolysis Pseudomonas Pseudomonas aeruginosa RNA Ralstonia Reporting Research Personnel Resistance Rhizobium radiobacter Ribosomes Role Signal Transduction Small RNA Sorting - Cell Movement Streptomyces Structure System Testing Thinking Vibrio Vibrio fischeri Virulence Virulence Factors Yersinia Yersinia enterocolitica Yersinia pestis base cell envelope density fascinate homoserine lactone luxR protein mutant octopine pathogen promoter quorum sensing receptor reconstitution response transcription factor tumor

项目摘要

DESCRIPTION (provided by applicant): The goal of this laboratory is continue to the develop the Agrobacterium-plant pathosystem as a leading model for host-microbe interactions and for pheromone-mediated bacterial communication. In the past 15 years, cell-cell communication via diffusible chemical signals has come to be appreciated as an important property of many bacteria, especially those associated with plant, animal, or human hosts. Among these signals, acyl-homoserine lactones (AHLs) are especially widespread among the proteobacteria. AHLs are generally synthesized by proteins resembling the Luxl protein of Vibrio fischeri, and detected by transcription factors resembling the V. ficsheri LuxR protein. My lab focuses on genetic and biochemical studies of several LuxR-type proteins. One of these systems, Tral and TraR of Agrobacterium tumefaciens regulate genes required for the conjugation and vegetative replication of a large tumor-inducing plasmid. Cepl and CepR of Burkholderia cenocepacia regulate many genes on several chromosomes and plasmid of this bacterium, while Yenl and YenR of Yersinia enterocolitica regulate an unknown number of genes, including yenS, which appears to encode a small regulatory RNA. We will continue to test the hypothesis that TraR and CepR require AHL for correct folding, and must bind this pheromone during their own synthesis on ribosomes. We will also study how the activating region or regions of TraR interact with RNA polymerase. We will identify target genes activated by CepR and YenR. YenR is unusual in that it is active as an apo-protein and its activity is inhibited by AHLs. We will try to reconstitute this inhibition in vitro. Finally, we will test the idea that yenS encodes a small non-coding RNA and determine whether this sRNA regulates the stability of target mRNAs by forming heteroduplexes. Direct targets of YenR and YenS will be identified and assessed for their roles in the biology and ecology of Y. enterocolitica and Y. pestis.

描述（由申请人提供）：该实验室的目标是继续开发农杆菌-植物病理系统，作为宿主-微生物相互作用和信息素介导的细菌通讯的主要模型。在过去 15 年中，通过可扩散化学信号进行的细胞间通讯已被视为许多细菌的重要特性，尤其是与植物、动物或人类宿主相关的细菌。在这些信号中，酰基高丝氨酸内酯（AHL）在变形菌中尤其普遍。 AHL 通常由类似于费氏弧菌 Luxl 蛋白的蛋白质合成，并由类似于费氏弧菌 LuxR 蛋白的转录因子检测。我的实验室专注于几种 LuxR 型蛋白质的遗传和生化研究。其中一个系统，根癌农杆菌的 Tral 和 TraR，调节大型肿瘤诱导质粒的接合和营养复制所需的基因。新洋葱伯克霍尔德菌的 Cep1 和 CepR 调节该细菌的几条染色体和质粒上的许多基因，而小肠结肠炎耶尔森氏菌的 Yenl 和 YenR 调节未知数量的基因，包括 YenS，它似乎编码一个小的调节 RNA。我们将继续测试这样的假设：TraR 和 CepR 需要 AHL 才能正确折叠，并且在核糖体上自身合成过程中必须结合这种信息素。我们还将研究 TraR 的激活区域如何与 RNA 聚合酶相互作用。我们将鉴定 CepR 和 YenR 激活的靶基因。 YenR 的不同寻常之处在于它作为脱辅基蛋白具有活性，并且其活性被 AHL 抑制。我们将尝试在体外重建这种抑制作用。最后，我们将测试yenS编码小非编码RNA的想法，并确定该sRNA是否通过形成异源双链体来调节靶mRNA的稳定性。将确定 YenR 和 YenS 的直接靶标，并评估它们在小肠结肠炎耶尔森氏菌和鼠疫耶尔森氏菌的生物学和生态学中的作用。