Hybrid Complete Protection Flourescence Molecular Tomography and X-ray CT

混合完全保护荧光分子断层扫描和 X 射线 CT

• 批准号: 7415088
• 负责人: RALPH WEISSLEDER, MD, PHD
• 金额: $ 36.74万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7415088
• 关键词: Accounting; Algorithms; Animals; Arts; Attention; Basic Science; Cell physiology; Chimeric Proteins; Collection; Data; Data Set; Dependence; Depth; Detection; Development; Diffuse; Dimensions; Disease; Engineering; Feasibility Studies; Fiber; Fluorescence; Fluorescent Dyes; Fluorochrome; Generations; Genomics; Goals; Heterogeneity; Hybrids; Image; Imagery; Imaging Techniques; Imaging technology; Lighting; Liquid substance; Malignant Neoplasms; Methods; Modality; Molecular; Molecular Probes; Numbers; Optical Tomography; Optics; Performance; Photons; Principal Investigator; Procedures; Property; Proteins; Proteomics; Publications; Reporting; Resolution; Sampling; Scheme; Science; Signal Transduction; Small Animal Imaging Systems; Source; Surface; System; Technology; Testing; Three-Dimensional Image; Time; Tissues; Weight; X-Ray Computed Tomography; X-Ray Tomography; animal tissue; base; charge coupled device camera; design; drug discovery; fluorescence imaging; gene function; improved; in vivo; interest; molecular imaging; mouse model; next generation; novel; novel strategies; optical imaging; physical model; pre-clinical; preclinical study; programs; prototype; reconstruction; tissue processing; tomography; treatment effect

DESCRIPTION (provided by applicant): The increasing availability of powerful fluorescent agents for reporting on functional and molecular tissue processes in-vivo opens new ways for detecting disease, characterizing cellular and sub-cellular processes and for assessing the effects of treatments. With this increased capacity for visualization conies a pressing need for highly advanced imaging technology that can accurately resolve in three dimensions and quantify the bio-distribution of such fluorochromes through dense tissues. Fluorescence macroscopy of entire animals and tissues is a most challenging and still largely unexplored field of imaging sciences, whose full potential is far from being reached. This proposal offers to develop a hybrid FMT system that combines the most advanced current developments on optical tomography with high-resolution X-ray Computed Tomography to offer unprecedented imaging capacity for small animal imaging. The system proposed is a fundamentally new design that assumes complete projection (360 degrees) illumination and detection, similarly to geometrical practices seen in X-ray CT. A CCD camera is used for obtaining high spatial sampling photon collection in the absence of previously utilized matching fluids or fibers in physical contact with tissue. As such, the proposed design and methods are not simply an incremental step forward compared to previous implementations but offer a new generation in terms of performance and experimental simplicity. While we expect the technology to find many small animal imaging applications, we are particularly interested in applying it for improving imaging in mouse models of cancer. We hypothesize that the proposed developments will yield a practical and highly efficient system that can become a method of choice in many preclinical studies involving in-vivo imaging of entire animals.

描述（由申请人提供）：强大的荧光剂的可用性增加用于报告功能性和分子组织过程的体内，为检测疾病，表征细胞和亚细胞过程的新方法和评估治疗的影响。随着可视化能力的增加，对高级成像技术的紧迫需求可以在三个维度上准确解析，并通过致密的组织量化此类荧光染料的生物分布。整个动物和组织的荧光宏观检查是成像科学领域最具挑战性的，但仍未开发的领域，其全部潜力远未达到。该建议提供了一个混合FMT系统，该系统将光学断层扫描上最先进的当前开发与高分辨率X射线计算机断层扫描相结合，为小动物成像提供前所未有的成像能力。该系统提出的是一种从根本上进行的新设计，它假定完全投影（360度）照明和检测，类似于X射线CT中看到的几何实践。 CCD摄像头用于在没有先前使用的匹配流体或与组织物理接触的匹配流体或纤维的情况下获得高空间采样光子收集。因此，与以前的实现相比，提出的设计和方法不仅是向前迈进的一步，而且在性能和实验简单方面提供了新一代。尽管我们希望该技术能够找到许多小型动物成像应用，但我们特别有兴趣将其应用于癌症的小鼠模型中。我们假设所提出的发展将产生一个实用且高效的系统，在许多临床前研究中，该系统可以成为一种涉及整个动物体内成像的临床前研究方法。