Study of gamma interferon agonists/mimetics

γ干扰素激动剂/模拟物的研究

• 批准号: 7638699
• 负责人: HOWARD M JOHNSON
• 金额: $ 10.08万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-06-01 至 2010-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7638699
• 关键词: Agonist; Antiviral Agents; Biological; C-terminal; CDKN1A gene; Cell physiology; Cells; Chemicals; Communicable Diseases; Development; Down-Regulation; Health; Histocompatibility Antigens Class II; Ifni; Immunotherapy; Interferon Type II; Interferons; Malignant Neoplasms; Mediating; Mediation; Methods; Modification; Molecular; Peptides; Proto-Oncogenes; Signal Transduction; System; Testing; Tumor Suppressor Genes; Up-Regulation; Virus Diseases; base; cancer cell; cytokine; mimetics; mouse model; oncoprotein p21

Gamma interferon (IFN_() is an essential cytokine for mediation of functions that are obligatory for good health, particularly that mediated through or maintained through CD4 T- helper 1 (Thl) cells. Further, IFN_, possesses direct antiviral and anticellular (antitumor) activity. Thus, the development of IFN1, mimetics has important implications for immune- therapy in infectious diseases and cancer. The Specific Aims listed below directly test both the rational development of such mimetics as well as their use in the mediation of IFN_, activity. 1. Synthesis of IFN1, peptide mimetics based on sequence and structural similarity to biologically active IFNI, C-terminal peptides. 2. Compare several methods for delivery of IFN_, peptides intracellularly, including chemical modification and use of molecular expression systems. 3. Determine signaling function of IFN_, peptide mimetics in terms of JAK/STAT activation, upregulation of tumor suppressor gene p21 wAF/cIP, and down regulation of neu/HER-2 protooncogene in cancer cells. 4. Determine cellular function of IFN_, peptide mimetics in terms of antiviral activity, anticellular (antitumor) activity, and upregulation of MHC class II molecules. 5. Determine biological activity of IFN_, mimetic peptides in mouse models of virus infection and cancer.

γ干扰素（IFN_()）是一种重要的细胞因子，可介导以下功能