Tissue Culture Biomechanical Core

组织培养生物力学核心

基本信息

批准号：
7407794
负责人：
VISWANATHAN NATARAJAN
金额：
$ 22.41万
依托单位：
UNIVERSITY OF CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-02-01 至 2013-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7407794
关键词：
Actins Adherens Junction Annona muricata coronin Atomic Force Microscopy Biomechanics CD44 gene Code Computer software Core Facility Cultured Cells Cytoskeletal Proteins Cytoskeleton Endothelial Cells Engineering Ensure Environment Equipment Focal Adhesions Fostering Functional disorder Genetic Polymorphism Genetically Engineered Mouse Goals Growth Factor Human Human Resources Hyperoxia Individual Inflammatory Laboratory Personnel Lung MAP Kinase Gene MYLK gene Measurement Measures Mechanical Stress Mediating Membrane Microdomains Microscopic Modeling Mus NADPH Oxidase Pathology Pathway interactions Permeability Phospholipase D Phosphorylation Phosphotransferases Process Protein Isoforms Protein Overexpression Proteins Proto-Oncogene Protein pp60 (c-src)RNA Splicing Regulation Research Personnel Resources Role Services Signal Transduction Stretching Structure Techniques Tight Junctions Training Transgenic Organisms Variant Vascular Endothelial Growth Factors Work caveolin 1 coronin protein design edg-1 Protein human EMS1 protein in vivo instrumentation lung injury paxillin polymerization programs protein kinase A kinase pulmonary artery endothelial cell research study rho software systems tissue culture

项目摘要

The Tissue Culture/Biomechanical Core (Core B) will provide critical support to Program Project Investigators while serving as a resource center and mechanism of integration between the five Projects. The Core facility will serve as a centralized resource unit providing laboratory personnel, expertise, and equipment to meet all human and murine endothelial cell needs for projects in the program. As all the five projects and Core D share a common theme of endothelial barrier regulation by the cytoskeleton, the major emphasis of this core will be to culture primary human pulmonary artery endothelial cells (HPAECs), human lung microvascular endothelial cells (HLMVECs), and isolate lung microvascular endothelial cells from various transgenic and genetically-altered murine models. The core has well trained personnel to process all the cell culture work and isolate lung microvascular ECs from murine models. The expertise and training we have developed during the last two cycles of this program project have defined the most effective mechanisms for culturing endothelial cells to assess permeability changes and/or pathologies. Another critical function of this Core will be facilitate experiments with cyclic stretch and atomic force microscopy (Core D) using dedicated instrumentation, computers and software systems. The close proximity of the tissue culture, biophysical and atomic force microscopy facilities will foster interaction between the investigators to develop and design experiments and interpret the reults. This will provide a unique environment where cell culture/biomechanical models and microscopic techniques will be refined and tailored to specific scientific needs of the projects. Thus, the tissue culture/biomechanical core facility is an integral component of the program project that ensures the most effective and economical use of the resources and expertise to meet the goals for each project.

组织培养/生物力学核心（核心B）将为程序项目提供关键支持研究人员在五个项目之间充当资源中心和整合机制。核心设施将作为一个集中资源部门，提供实验室人员，专业知识和满足计划中所有人类和鼠的内皮细胞需求的设备。作为五个项目和核心D共享细胞骨架的内皮屏障调节的共同主题，主要是该核心的重点将是培养原代人肺动脉内皮细胞（HPAEC），人类肺微血管内皮细胞（HLMVEC），并分离肺微血管内皮细胞各种转基因和遗传改变的鼠模型。核心有训练有素的人员来处理所有细胞培养的工作和分离鼠模型的肺微血管EC。我们的专业知识和培训在该计划项目的最后两个周期中已经开发了最有效的培养内皮细胞评估渗透性变化和/或病理的机制。其他该核心的关键功能将促进循环拉伸和原子力显微镜的实验（核心D）使用专用仪器，计算机和软件系统。近距离组织培养，生物物理和原子力显微镜设施将促进调查人员开发和设计实验并解释这些实验。这将提供独特的细胞培养/生物力学模型和微观技术的环境将被完善，并且根据项目的特定科学需求量身定制。因此，组织培养/生物力学核心设施是计划项目的整体组成部分，以确保最有效，最经济地使用资源和专业知识以满足每个项目的目标。