Tissue Culture Biomechanical Core

组织培养/生物力学核心（核心B）将为计划项目提供关键支持 调查员同时充当五个项目之间的资源中心和整合机制。 核心设施将作为一个集中资源单位，提供实验室人员、专业知识和 设备，以满足所有人类和小鼠内皮细胞的项目在该计划的需求。作为所有五个 项目和核心D有一个共同的主题，即细胞骨架对内皮屏障的调节，主要是 本核心的重点将是培养原代人肺动脉内皮细胞（HPAECs），人肺动脉内皮细胞（HPAECs）， 肺微血管内皮细胞（HLMVECs），并从 各种转基因和遗传改变的鼠模型。核心有训练有素的人员处理所有 细胞培养起作用并从鼠模型中分离肺微血管内皮细胞。我们的专业知识和培训 在本计划项目的最后两个周期中， 培养内皮细胞以评估渗透性变化和/或病理的机制。另一 这个核心的关键功能将是促进循环拉伸和原子力显微镜的实验 (Core D）使用专用仪器、计算机和软件系统。的紧密接近 组织培养、生物物理和原子力显微镜设施将促进 研究人员开发和设计实验并解释结果。这将提供一个独特的 细胞培养/生物力学模型和显微技术将得到完善的环境， 根据项目的具体科学需要量身定制。因此，组织培养/生物力学核心设施是一个 该计划项目的组成部分，确保最有效和最经济的使用， 资源和专业知识，以满足每个项目的目标。

The Tissue Culture/Biomechanical Core (Core B) will provide critical support to Program Project Investigators while serving as a resource center and mechanism of integration between the five Projects. The Core facility will serve as a centralized resource unit providing laboratory personnel, expertise, and equipment to meet all human and murine endothelial cell needs for projects in the program. As all the five projects and Core D share a common theme of endothelial barrier regulation by the cytoskeleton, the major emphasis of this core will be to culture primary human pulmonary artery endothelial cells (HPAECs), human lung microvascular endothelial cells (HLMVECs), and isolate lung microvascular endothelial cells from various transgenic and genetically-altered murine models. The core has well trained personnel to process all the cell culture work and isolate lung microvascular ECs from murine models. The expertise and training we have developed during the last two cycles of this program project have defined the most effective mechanisms for culturing endothelial cells to assess permeability changes and/or pathologies. Another critical function of this Core will be facilitate experiments with cyclic stretch and atomic force microscopy (Core D) using dedicated instrumentation, computers and software systems. The close proximity of the tissue culture, biophysical and atomic force microscopy facilities will foster interaction between the investigators to develop and design experiments and interpret the reults. This will provide a unique environment where cell culture/biomechanical models and microscopic techniques will be refined and tailored to specific scientific needs of the projects. Thus, the tissue culture/biomechanical core facility is an integral component of the program project that ensures the most effective and economical use of the resources and expertise to meet the goals for each project.