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Tissue Culture Biomechanical Core

组织培养生物力学核心

基本信息

批准号：
8214993
负责人：
VISWANATHAN NATARAJAN
金额：
$ 30.06万
依托单位：
UNIVERSITY OF ILLINOIS AT CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-02-01 至 2013-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8214993
关键词：
Actins Adherens Junction Atomic Force Microscopy Biomechanics CD44 gene Cell Culture Techniques Code Computer software Core Facility Cytoskeletal Proteins Cytoskeleton Endothelial Cells Engineering Ensure Environment Equipment Focal Adhesions Fostering Functional disorder Genetic Polymorphism Genetically Engineered Mouse Goals Growth Factor Human Human Resources Hyperoxia Individual Inflammatory Laboratory Personnel Lung MAP Kinase Gene MYLK gene Measurement Measures Mechanical Stress Mediating Membrane Microdomains Microscopic Modeling Mus NADPH Oxidase Pathology Pathway interactions Permeability Phospholipase D Phosphorylation Phosphotransferases Process Protein Isoforms Proteins Proto-Oncogene Protein pp60 (c-src)RNA Splicing Regulation Research Personnel Resources Role Services Signal Transduction Stretching Structure Techniques Tight Junctions Training Transgenic Organisms Variant Vascular Endothelial Growth Factors Work caveolin 1 coronin protein design edg-1 Protein human EMS1 protein in vivo instrumentation lung injury meetings overexpression paxillin polymerization programs protein kinase A kinase pulmonary artery endothelial cell research study rho software systems tissue culture

项目摘要

The Tissue Culture/Biomechanical Core (Core B) will provide critical support to Program Project Investigators while serving as a resource center and mechanism of integration between the five Projects. The Core facility will serve as a centralized resource unit providing laboratory personnel, expertise, and equipment to meet all human and murine endothelial cell needs for projects in the program. As all the five projects and Core D share a common theme of endothelial barrier regulation by the cytoskeleton, the major emphasis of this core will be to culture primary human pulmonary artery endothelial cells (HPAECs), human lung microvascular endothelial cells (HLMVECs), and isolate lung microvascular endothelial cells from various transgenic and genetically-altered murine models. The core has well trained personnel to process all the cell culture work and isolate lung microvascular ECs from murine models. The expertise and training we have developed during the last two cycles of this program project have defined the most effective mechanisms for culturing endothelial cells to assess permeability changes and/or pathologies. Another critical function of this Core will be facilitate experiments with cyclic stretch and atomic force microscopy (Core D) using dedicated instrumentation, computers and software systems. The close proximity of the tissue culture, biophysical and atomic force microscopy facilities will foster interaction between the investigators to develop and design experiments and interpret the reults. This will provide a unique environment where cell culture/biomechanical models and microscopic techniques will be refined and tailored to specific scientific needs of the projects. Thus, the tissue culture/biomechanical core facility is an integral component of the program project that ensures the most effective and economical use of the resources and expertise to meet the goals for each project.

组织培养/生物力学核心（核心B）将为项目提供关键支持