Differential Gene Expression in Keloids

瘢痕疙瘩代表了一种阴险的疾病过程，主要影响年轻的少数民族患者离开他们 有缺陷，偶尔也有残疾这项研究旨在解决观察到的问题， 与正常来源的皮肤细胞相比， 皮肤细胞以应激的形式受到血清刺激和身体劳损。将对这一现象进行研究 通过关注TGF-β 2基因对血清刺激的不同转录反应， 角质形成细胞本申请的三个具体目的是确定启动子元件 对于这种不同的反应，确定介导反应的信号转导途径，并确定 可能与TGF-β 2协同调节并促成瘢痕疙瘩的其他基因 表型除了回答有关瘢痕疙瘩生物学的重要问题，该应用程序还将服务于 !为主要研究者的学术生涯提供支持。他展示了一个 长期致力于生物医学研究的记录，并选择了伤口愈合和外科手术作为他的研究领域。 希望追求。他最初的目标是建立一个伤口愈合实验室， 校外资助首席研究员的长期目标是利用他的知识， 研究伤口愈合，以更好地了解皮肤的生物学。这些信息可以转换为 通过治疗慢性伤口或过度疤痕的临床应用， 组织工程合成人工皮肤，可应用于临床重建问题， 包括烧伤职业发展计划是详细的，旨在教育校长 研究者在适当的，道德的研究行为。已经制定了提高具体技能的计划 以促进他的职业发展。这些包括实验设计，granesthetic，与 外科和科学界，以及手稿准备和审查。该项目将在 在斯坦福大学医学院一位非常成功的导师的指导下。这是其中一 领先的生物医学研究机构在全国，它已经实施了多个计划，旨在 改善转化医学。该机构拥有开展这一项目所需的一切资源， 此外还有多位该领域的专家提供咨询。

Keloids represent an insidious disease process that afflicts primarily young, minority patients leaving them with disfigurement, and occasionally, disability. This research application seeks to address the observation that keloid-derived skin cells appear to have an aberrant genotypic response compared to normal-derived skin cells to stress in the form of serum stimulation and physical strain. This phenomenon will be examined by focusing on the differential transcriptional response of the TGF-beta2 gene to serum stimulation in keratinocytes. The three specific aims of this application are to determine the promoter elements responsible for this differential response, identify the signal transduction pathways mediating the response, and identify additional genes that may be coordinately regulated with TGF-beta2 and contributing to the keloid Iphenotype. In addition to answering important questions about keloid biology, this application will also serve !to provide the nit a support for the academic career of the Principal Investigator. He has demonstrated a long record of commitment to biomedical research and has chosen wound healing and surgery as the field he wishes to pursue. His initial goal is to establish a laboratory in wound healing that is fully supported through extramural funding. The long term goal of the Principal Investigator is to use the knowledge derived from his studies in wound healing to better understand the biology of skin. This information can then translated into clinical applications through the treatment of chronic wounds or excessive scarring, and can also be used in tissue engineering to synthesize artificial skin that can be applied to clinical problems in reconstruction, including burns. A career development program is detailed that is designed to educate the Principal Investigator in the proper, ethical conduct of research. Plans have been developed to improve specific skills to promote his career development. These include experimental design, grantsmanship, interactions with the surgical and scientific community, and manuscript preparation and review. This project will be carried out under the tutelage of a highly successful Mentor at Stanford University Medical School. This is one of the leading biomedical research institutions in the nation, and it has implemented multiple programs aimed at improving translational medicine. The institution has every resource needed to carry out this project in addition to a number of experts in the field to provide advice.