JEM-3200FS Field Emission Electron Microscope

描述（由申请人提供）：长期目标是获得一台300千伏场发射电子显微镜（FETEM），该显微镜将增强、补充和增加成像能力，包括厚样品、EELS、STEM和EDS的低温电子显微镜。目前，美国西北大学（NU）的研究人员还没有类似的中电压显微镜系统。申请资金用于购买具有多个分析模块的JEOL 3200FS FETEM系统。我们将使用3200FS: 1)结合我们的共聚焦系统执行相关的光学和电子显微镜，2)成像和分析独特探针和纳米颗粒的细胞摄取，以及3)捕获倾斜系列厚的冷冻水合和树脂包埋样品进行断层扫描。3200FS系统可以成像厚样品（400nm-1(m)）。该FETEM提供可靠的成像和光谱分析使用的标本持有人，可以容纳各种各样的标本类型。加上EDS， EELS和STEM的分析功能，使该系统非常强大-能够调查和表征许多生物系统，这是我们目前的TEM无法做到的。

DESCRIPTION (provided by applicant): The long-term objective is to obtain a 300 kV field emission electron microscope (FETEM) that will enhance, complement, and increase repertoire of imaging abilities to include cryoelectron microscopy of thick samples, EELS, STEM, and EDS. Presently there is no comparable intermediate voltage microscope system that is available to researchers at Northwestern University (NU). Funds are requested to purchase a JEOL 3200FS FETEM system that has several analytical modules. We will use the 3200FS to: 1) perform correlative light and electron microscopy in conjunction with our confocal systems, 2) image and analyze the cellular uptake of unique probes and nanoparticles, and 3) capture tilt-series of thick frozen-hydrated and resin-embedded samples for tomography. The 3200FS system can image thick samples (400nm-1(m). This FETEM provides reliable imaging and spectral analysis using specimen holders that can accommodate a wide variety of specimen types. Adding the analytical functions of EDS, EELS, and STEM makes this system extremely powerful - capable of investigating and characterizing many biological systems that are impossible with our current TEM. Together, the eight major users listed in this proposal have over 20 currently funded NIH grants totaling more than $5M. Their research projects encompass a wide array of developmental, cell, and structural biology studies including: 1) estrogen-induced plasticity of brain synapses, 2) cellular uptake of oligonucleotide- modified nanostructures, 3) the structure, function, and organization of viral surface glycoproteins, 4) structural studies of DNA topoisomerases, and 5) peptide amphiphile self-assembly. These and many other projects will significantly benefit from the additional imaging and analytical features of the 3200FS - features that our present TEM lacks. Results obtained from these studies will have significant impact on public health research in areas such as revealing molecular/cellular targets for novel therapeutic strategies for multiple brain/behavior disorders; designing/testing innovative nanoscale technologies that significantly improve cancer prevention, diagnosis, and treatment; and understanding the process of viral entry into host cells. Based on staff experience and on demonstrations of two 300 kV FETEM units (from FEI and JEOL), we have determined that the JEOL 3200FS system best suits our needs. This equipment will be administered by BIF on the NU Evanston campus. BIF is a shared-use facility that supports over 470 Northwestern researchers. BIF is also available to neighboring academic and health care institutions, and outside individuals.