SCIEX 4000 QTRAP Mass Spectrometer

描述(由申请人提供)：这是一份新的申请，申请资金支持购买SCIEX 4000 QTRAP质谱仪，以增强加州大学圣迭戈分校脂质分析核心实验室的功能。这个核心实验室已经运行了5年，并不断开发新的方法学，逐步推进LC/MS在血脂测定和标准化定量中的应用。它对于成功资助两个NIH计划项目赠款(MUSC的神经鞘脂在癌症中的作用和合作机构中的肝细胞凋亡)、NIH/NCRR生物医学研究卓越中心(初始和竞争续订赠款)以及作为MUSC霍林斯癌症中心核心设施的多个个人研究项目至关重要。2006年，Lipid Analytic核心实验室记录了23名MUSC用户、17名MUSC以外用户和5名国际用户的11,500多次血脂检测。核心实验室目前依赖于两个LC/MS设置-两个都已达到容量。其中一台已使用14年以上，已超过其正常使用寿命，不再受制造商支持。因此，一个由17个主要用户组成的核心小组，代表了MUSC的7个部门，正在申请这笔赠款，以获得SCIEX 4000 QTRAP质谱仪的配置，该质谱仪具有专用的高效液相色谱，用于样品传递和成分分离。这一请求得到了全国顶尖生物医学研究机构具有代表性的7名外部用户样本的支持。分析核心实验室的技术专长包括一名具有丰富的定性和定量脂类化学的全职教员和3名工作人员。全面的行政计划确保适当的管理、监督、公平的访问和合规。强有力的机构承诺确保所要求的仪器和整体设施的长期运行和维护。

DESCRIPTION (provided by applicant): This is a new application requesting funds to support the acquisition of a SCIEX 4000 QTRAP mass spectrometer to enhance the function of the Lipid Analytic Core Lab at MUSC. This core lab has been in operation for 5 years, and has continually developed novel methodology and progressively advanced the application of LC/MS for the determination of lipids and standardized quantitation. It has been critical to the successful funding of two NIH program project grants (on sphingolipids in cancer at MUSC and apoptosis in liver cells at a collaborating institution), an NIH/NCRR Center of Biomedical Research Excellence (initial and competing renewal grants), and multiple individual investigator research projects as a core facility of the Hollings Cancer Center at MUSC. In 2006, the Lipid Analytic Core Lab logged more than 11,500 lipid assays for 23 MUSC users, 17 users outside MUSC, and 5 international users. The core lab currently relies on two LC/MS set ups ---- both are being used to capacity. One has been in service more than 14 years, has passed its normal life expectancy, and is no longer supported by the manufacturer. Therefore, a core group of 17 major users, representing 7 departments at MUSC, are requesting this grant to acquire a SCIEX 4000 QTRAP mass spectrometer in configuration with a dedicated HPLC for sample delivery and component separation. This request is supported by a representative sample of 7 outside users at premier biomedical research institutions across the nation. Technical expertise within the Analytic Core Lab includes a fulltime faculty member with extensive qualitative and quantitative lipid chemistry and 3 staff members. A comprehensive Administrative Plan ensures appropriate management, oversight, equitable access, and compliance. Strong institutional commitment ensures long term operation and maintenance of the requested instrument and overall facility.