Magnetic Circular Dichroism/Circular Discroism System

描述(由申请人提供)：我们正在申请共享仪器赠款，以购买磁性圆二色/圆二色(MCD/CD)系统，包括JASCO-815和JASCO-730分光光度计，用于广泛的血红素蛋白和其他生物医学研究。这个MCD/CD系统将被用来扩展以前用陈旧的JASCO-500所做的工作，并开始超出旧仪器能力的研究。在以下几种情况下，购买该系统是合理的： *MCD/CD光谱与电子顺磁共振(EPR)相结合，是对涉及过渡金属中心的许多生物系统进行结构分析的非常强大的工具。我们扩大了研究范围，现在研究了多达20种不同的血红蛋白和几种铁硫蛋白。J-815 PLUS J-730 MCD/CD系统覆盖160-2000纳米范围，允许对目标大分子进行全面表征，并利用德克萨斯大学医学院的EPR设施增强正在进行的项目。 *所要求的MCD/CD能力旨在与德克萨斯大学休斯顿医学院结构生物学中心的现有核磁共振和结晶学能力相结合。MCD/CD系统将扩大与墨西哥湾沿岸联盟有关的广泛用户可用的光谱工具，包括休斯顿地区分子生物物理培训计划，从而加强德克萨斯大学医学院、莱斯大学、贝勒医学院和休斯顿大学科学家之间的科学互动。 *德克萨斯大学休斯顿医学院和莱斯大学在生化和生物物理研究中稳步增加MCD/CD技术的使用。为了保证可靠的MCD/CD设施用于持续的研究和培训目的，我们选择购买JASCO光谱仪，因为它们的多功能、耐用性和易用性。

DESCRIPTION (provided by applicant): We are requesting a shared instrument grant to purchase a Magnetic Circular Dichroism/ Circular Dichroism (MCD/CD) system, including JASCO-815 and JASCO-730 spectrophotometers, to be used for a wide range of hemeproteins and other biomedical research. This MCD/CD system will be used to extend work previously done with an aged JASCO-500 and to begin studies beyond the older instrument's capabilities. Purchase of this system is justified on several cases: * MCD/CD spectroscopy, in combination with electron paramagnetic resonance (EPR), is a very powerful tool in structural analysis of the many biological systems that involve transition metal centers. We have expanded our research scope and now study as many as two dozens different hemeproteins and a few iron-sulfur proteins. The J-815 plus J-730 MCD/CD system, covering the 160-2000 nm range, permits full characterization of the target macromolecules and enhances ongoing projects using the EPR facility at the UT Medical School. * The requested MCD/CD capability is designed to integrate with existing NMR and crystallographic capabilities of the Structural Biology Center at the UT-Houston Medical School. The MCD/CD system will expand the spectroscopic tools available to a wide spectrum of users associated with the Gulf Coast Consortium, including the Houston Area Molecular Biophysics Training Program, thus strengthening scientific interactions among scientists at the UT Medical School, Rice University, Baylor College of Medicine and the University of Houston. * Steadily increased usage of MCD/CD techniques in biochemical and biophysical studies at the University of Texas Houston Medical School and at Rice University. To guarantee a reliable MCD/CD facility for ongoing research and training purposes, we chose to buy JASCO spectrometers for their versatile performance, durability and ease of use.