Magnetic Circular Dichroism/Circular Discroism System

磁圆二色性/圆二色性系统

• 批准号: 7389761
• 负责人: AH-LIM TSAI
• 金额: $ 31.36万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-02-01 至 2009-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7389761
• 关键词: Area; Biochemical; Biological; Biomedical Research; Biophysics; Circular Dichroism; Circular Dichroism Spectroscopy; Electron Spin Resonance Spectroscopy; Grant; Hemeproteins; Iron-Sulfur Proteins; Medicine; Molecular; Performance; Purpose; Range; Research; Research Training; Rice; Scientist; System; Techniques; Texas; Training Programs; Transition Elements; Universities; Work; aged; circular magnetic dichroism; college; design; gulf coast; instrument; macromolecule; medical schools; structural biology; tool

DESCRIPTION (provided by applicant): We are requesting a shared instrument grant to purchase a Magnetic Circular Dichroism/ Circular Dichroism (MCD/CD) system, including JASCO-815 and JASCO-730 spectrophotometers, to be used for a wide range of hemeproteins and other biomedical research. This MCD/CD system will be used to extend work previously done with an aged JASCO-500 and to begin studies beyond the older instrument's capabilities. Purchase of this system is justified on several cases: * MCD/CD spectroscopy, in combination with electron paramagnetic resonance (EPR), is a very powerful tool in structural analysis of the many biological systems that involve transition metal centers. We have expanded our research scope and now study as many as two dozens different hemeproteins and a few iron-sulfur proteins. The J-815 plus J-730 MCD/CD system, covering the 160-2000 nm range, permits full characterization of the target macromolecules and enhances ongoing projects using the EPR facility at the UT Medical School. * The requested MCD/CD capability is designed to integrate with existing NMR and crystallographic capabilities of the Structural Biology Center at the UT-Houston Medical School. The MCD/CD system will expand the spectroscopic tools available to a wide spectrum of users associated with the Gulf Coast Consortium, including the Houston Area Molecular Biophysics Training Program, thus strengthening scientific interactions among scientists at the UT Medical School, Rice University, Baylor College of Medicine and the University of Houston. * Steadily increased usage of MCD/CD techniques in biochemical and biophysical studies at the University of Texas Houston Medical School and at Rice University. To guarantee a reliable MCD/CD facility for ongoing research and training purposes, we chose to buy JASCO spectrometers for their versatile performance, durability and ease of use.

描述（由申请人提供）：我们正在申请一项共享仪器赠款，以购买磁性圆二色性/圆二色性（MCD/CD）系统，包括JASCO-815和JASCO-730荧光计，用于广泛的血红素蛋白和其他生物医学研究。该MCD/CD系统将用于扩展以前用老化的JASCO-500进行的工作，并开始超出旧仪器能力的研究。在以下几种情况下，购买该系统是合理的： * MCD/CD光谱与电子顺磁共振（EPR）相结合，是许多涉及过渡金属中心的生物系统结构分析中非常强大的工具。我们已经扩大了我们的研究范围，现在研究多达20种不同的血红素蛋白和一些铁硫蛋白。J-815加上J-730 MCD/CD系统，覆盖160-2000 nm范围，允许对目标大分子进行全面表征，并使用UT医学院的EPR设施增强正在进行的项目。 * 所要求的MCD/CD能力旨在与UT休斯顿医学院结构生物学中心现有的NMR和晶体学能力相结合。MCD/CD系统将扩大与墨西哥湾沿岸联盟有关的广泛用户可用的光谱工具，包括休斯顿地区分子生物物理学培训计划，从而加强UT医学院，赖斯大学，贝勒医学院和休斯顿大学科学家之间的科学互动。 * 在德克萨斯大学休斯顿医学院和莱斯大学的生物化学和生物物理研究中，MCD/CD技术的使用稳步增加。为了保证可靠的MCD/CD设备用于持续的研究和培训目的，我们选择购买JASCO光谱仪，因为它们具有多功能性，耐用性和易用性。