ELECTRON PARAMAGNETIC RESONANCE (EPR) SPECTROMETER

我们申请共享仪器拨款，购买一台布鲁克EMX系列电子顺磁共振（EPR）光谱仪，用于生物化学和医学研究。该系统将用于逐步淘汰老化的瓦里安E-6机器在莱斯大学，生物化学和细胞生物学。对该光谱仪的需求在多个层面上是合理的：* EPR技术在休斯敦德克萨斯大学医学院和莱斯大学生物化学和生物物理研究中的使用稳步增加。为了保证可靠的EPR设施用于正在进行的研究和培训目的，我们选择购买布鲁克EMX EPR光谱仪，因为它的耐用性和使用寿命。* EPR是一个强大的工具，在许多生物系统的结构分析，涉及有机自由基和过渡金属中心。这种EPR能力将与UT-Houston医学院的结构生物学中心整合，以补充现有的核磁共振（NMR）设施，并配合该中心即将招聘的x射线晶体学家。*所申请的EPR系统将继续并扩大服务范围，以广泛的用户加入休斯顿地区分子生物物理训练计划，从而加强UT医学院、莱斯大学、贝勒医学院和休斯顿大学的科学家之间的科学互动。

We apply for a shared instrument grant to purchase a Bruker EMX series electron paramagnetic resonance (EPR) spectrometer to be used for biochemical and medical research. This system will be used to gradually phase out the aged Varian E-6 machine at Rice University, Biochemistry and Cell Biology. Demand for this spectrometer is justified at multiple levels: * A steady increase in usage of EPR technologies in biochemical and biophysical studies at the University of Texas Medical School at Houston and at Rice University. To guarantee a reliable EPR facility for ongoing research and training purposes, we chose to buy the Bruker EMX EPR spectrometer for its durability and use of use. * EPR is a powerful tool in the structural analysis of the many biological systems that involve organic radicals and transition metal centers. This EPR capability will integrate with the Structural Biology Center at the UT-Houston Medical School to complement the existing nuclear magnetic resonance (NMR) facility and with the Center's imminent recruitment of a X-ray crystallographer. * The quested EPR system will continue and expand service to a wide spectrum of users joining the Houston Area Molecular Biophysical Training Program, thus strengthening the scientific interactions among scientists at UT Medical School, Rice University, Baylor College of Medicine and University of Houston.