Administrative Core

为了更好地了解HIV感染期间口腔的独特性以及随后的 治疗，这个项目将定义HIV、特定可溶性宿主之间的功能关系 防御分子和口腔细菌。以项目1(改建)获得的数据为基础 是艾滋病毒中的唾液蛋白质组)，项目2(艾滋病毒和HAART对口腔微生物多样性的影响 和定居，以及项目3(艾滋病毒对胃肠道中可溶性介质和微生物的影响)， 这个项目的重点是唾液中的抗细菌和抗病毒活性。需要解决的问题 包括(1)从口腔对艾滋病毒的反应中可以学到什么，这可能是有用的 对于其他粘膜部位？(2)宿主防御分子和细胞因子是如何在 HIV感染和后续治疗？(3)抗病毒和抗菌活性如何变化 以及(4)口腔、肠道和口腔中HIV-1变异的比较。 可能受当地宿主因素影响的血液。最终，我们希望了解为什么直肠 艾滋病毒的传播比口头传播更有效，以及这些信息是如何 可以用来降低艾滋病毒感染率。为了实现这些目标，我们有 提出了以下具体目标： 具体目标1.量化已知相互作用的可溶性先天宿主因子的子集 同时携带艾滋病毒和细菌 具体目的2.定量测定来自HIV-的样品的抗HIV和抗菌活性， HIV+、HIV+/HAART受试者以及单个纯化蛋白 并确定口腔和直肠组织在易感性方面是否存在差异 HIV在体外的感染。 具体目标3.描述中国艾滋病病毒携带者的遗传和表型特征 口腔、血液和胃肠道。

To better understand the uniqueness of the oral cavity during HIV infection and subsequent therapy, this project will define the functional relationships among HIV, specific soluble host defense molecules and oral bacteria. Building on the data obtained from Projects 1 (Alteration is the salivary proteome in HIV), Project 2 (Effect of HIV and HAART on oral microbial diversity and colonization, and Project 3 (Effect of HIV on soluble mediators and microbes in the Gl tract), this project focuses on antibacterial and antiviral activities in saliva. Questions to be addressed include (1) what can be learned from how the oral cavity responds to HIV that could be useful for other mucosal sites?, (2) how host defense molecules and cytokines are modulated during HIV infection and subsequent therapy?, (3) how antiviral and antibacterial activities change during HIV infection?, and (4) a comparison of the HIV-1 variants in the oral cavity, gut, and blood that may be influenced by local host factors. Ultimately we hope to understand why rectal transmission of HIV is much more efficient than oral transmission and how this information could be used to decrease the rate of HIV infection. In order to accomplish these goals we have proposed the following Specific Aims: Specific Aim 1. To quantitate a subset of soluble innate host factors that are known to interact with both HIV and bacteria Specific Aim 2. To quantitate the anti-HIV and antibacterial activity of samples from HIV-, HIV+, and HIV+/HAART subjects as well as from individual purified proteins and to determine whether oral and rectal tissues differ in their susceptibility to HIV infection in vitro. Specific Aim 3. To characterize genetic and phenotypic features of the HIV reservoir in the oral cavity, blood, and gastrointestinal tract.