Lung Desease Models Core

肺部疾病模型核心

基本信息

批准号：
7476122
负责人：
STEPHEN Lloyd TILLEY
金额：
$ 21.24万
依托单位：
UNIV OF NORTH CAROLINA CHAPEL HILL
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-03-01 至 2013-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7476122
关键词：
Acute Airway Resistance Allergens Allergic Animal Model Animals Antigens Asthma Biochemical Biology Breeding Cell Count Chronic Computers Data Development Disease Disease model Endotoxins Environmental Pollutants Eosinophilia Evaluation Exposure to Formalin Freezing Genes Genetic Goals Harvest Histology Human Immunobiology Individual Inflammation Intervention Laboratory mice Lipids Lung Lung Inflammation Lung Lavage Fluid Lung diseases Measurable Measurement Measures Mechanics Messenger RNA Mission Modeling Molecular Mouse Strains Mucous body substance Mus National Institute of Allergy and Infectious Disease Numbers Obstruction Paralysed Pathogenesis Peripheral Phase Phenotype Physiological Production Proteins Protocols documentation Public Health Range Research Personnel Resistance Resources Role Serum Services Signal Pathway Structure of parenchyma of lung Technical Expertise Testing Tissue Banks Tissues Toxic effect Transgenic Organisms Ventilator Wild Type Mouse Work airway hyperresponsiveness airway inflammation airway remodeling design embryonic stem cell environmental agent genetic technology human disease human study improved in vivo interest methacholine mouse model novel protein expression response

项目摘要

The long-term objective of this core is to provide investigators interested in environmental lung disease the resources and technical expertise required to conduct in vivo mechanistic studies in mice. The following core services will be provided: 1) importation and exportation of mice, 2) breeding of wild type mice for mechanistic studies, 3) breeding and genotying of genetically manipulated mouse lines for use in mechanistic studies, 4) development of allergic mice using both acute and chronic sensitization/challenge protocols, 5) exposure of animals to model environmental pollutants, 6) treatment interventions to test the efficacy, toxicity, and mechanisms of action of novel therapies, and 7) physiological phenotyping of mice after the induction of environmental lung disease for evaluation of airway resistance, compliance, and airway hyperresponsiveness to methacholine. This core supports the mission of NIAID to study genetic and environmental interactions as they relate to environmental lung disease. Mechanistic studies in animals, particularly the use of genetically manipulated mice, is essential for the development of new therapies to improve public health.

该核心的长期目标是为对环境肺部疾病感兴趣的研究人员在小鼠中进行体内机械研究所需的资源和技术专长。下列将提供核心服务：1）进口和出口小鼠，2）野生型小鼠的育种机械研究，3）遗传操纵小鼠系的繁殖和基因构成用于用于机械研究，4）使用急性和慢性敏化/挑战的过敏小鼠的发展方案，5）动物暴露于建模环境污染物，6）治疗干预措施以测试新疗法的功效，毒性和作用机制，以及7）小鼠的生理表型诱导环境肺部疾病以评估气道阻力，合规性和气道对甲基胆碱的反应性过高。该核心支持NIAID的使命研究遗传和与环境肺部疾病有关的环境相互作用。动物的机械研究，特别是使用遗传操纵小鼠的使用，对于开发新疗法至关重要改善公共卫生。