Lung Desease Models Core

肺部疾病模型核心

• 批准号: 7977207
• 负责人: STEPHEN Lloyd TILLEY
• 金额: $ 22.16万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-03-01 至 2013-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7977207
• 关键词: Acute; Airway Resistance; Allergens; Allergic; Animal Model; Animals; Antigens; Asthma; Biochemical; Biology; Breeding; Cell Count; Chronic; Computers; Data; Development; Disease; Disease model; Endotoxins; Environmental Pollutants; Eosinophilia; Evaluation; Exposure to; Formalin; Freezing; Genes; Genetic; Goals; Harvest; Histology; Human; Immunobiology; Individual; Inflammation; Intervention; Laboratory mice; Lipids; Lung; Lung Inflammation; Lung Lavage Fluid; Lung diseases; Measurable; Measurement; Measures; Mechanics; Messenger RNA; Mission; Modeling; Mouse Strains; Mucous body substance; Mus; National Institute of Allergy and Infectious Disease; Obstruction; Paralysed; Pathogenesis; Peripheral; Phase; Phenotype; Physiological; Production; Proteins; Protocols documentation; Public Health; Research Personnel; Resistance; Resources; Role; Serum; Services; Signal Pathway; Structure of parenchyma of lung; Technical Expertise; Testing; Tissue Banks; Tissues; Toxic effect; Transgenic Organisms; Ventilator; Wild Type Mouse; Work; airway hyperresponsiveness; airway inflammation; airway remodeling; design; efficacy testing; embryonic stem cell; environmental agent; genetic technology; human disease; improved; in vivo; interest; meetings; methacholine; molecular phenotype; mouse model; novel; protein expression; response

The long-term objective of this core is to provide investigators interested in environmental lung disease the resources and technical expertise required to conduct in vivo mechanistic studies in mice. The following core services will be provided: 1) importation and exportation of mice, 2) breeding of wild type mice for mechanistic studies, 3) breeding and genotying of genetically manipulated mouse lines for use in mechanistic studies, 4) development of allergic mice using both acute and chronic sensitization/challenge protocols, 5) exposure of animals to model environmental pollutants, 6) treatment interventions to test the efficacy, toxicity, and mechanisms of action of novel therapies, and 7) physiological phenotyping of mice after the induction of environmental lung disease for evaluation of airway resistance, compliance, and airway hyperresponsiveness to methacholine. This core supports the mission of NIAID to study genetic and environmental interactions as they relate to environmental lung disease. Mechanistic studies in animals, particularly the use of genetically manipulated mice, is essential for the development of new therapies to improve public health.

该核心的长期目标是为对环境性肺病感兴趣的研究人员提供 开展小鼠体内机制研究所需的资源和技术专长。以下 核心服务将提供：1）小鼠进出口，2）野生型小鼠繁殖， 机制研究，3）用于以下的遗传操作的小鼠品系的育种和基因分型： 机制研究，4）使用急性和慢性致敏/激发的过敏小鼠的发展 方案，5）动物暴露于模型环境污染物，6）治疗干预，以测试 新疗法的功效、毒性和作用机制，以及7）小鼠的生理表型 在诱导环境性肺病后评估气道阻力、顺应性和气道 对乙酰甲胆碱过敏该核心支持NIAID的使命，研究遗传和 环境相互作用，因为它们与环境肺病有关。在动物中进行的机制研究， 特别是使用基因操作的小鼠，对于开发新的治疗方法至关重要， 改善公共卫生。