核心 C——氧化应激的生物标志物-猫眼课题宝

核心 C——氧化应激的生物标志物

批准号：

7454289

负责人：

Valerian E Kagan

金额：

$14.05万

依托单位：

UNIVERSITY OF PITTSBURGH AT PITTSBURGH

依托单位国家：

美国

项目类别：

财政年份：

资助国家：

美国

项目状态：

未结题

起止时间：

至

来源：

https://reporter.nih.gov/project-details/7454289

关键词：

4-anisyltetrazolium blue 8-hydroxy-2&apos -deoxyguanosine A549 Acetates Acetic Acid Acetic Acids Acetone Acetonitriles Acids Acridines Activated Charcoal Acute Lung Injury Affect Aftercare Albumins Aliquot Alkaline Phosphatase Animals Antioxidants Appearance Area Area Under Curve Arts Ascorbic Acid Azides Bee Venoms Binding Biological Biological Assay Biological Markers Biological Models Biological Sciences Biotin Biotinylation Boston Brain Buffers Butylated Hydroxytoluene Caliber Calibration Carbon Cardiolipins Cell Survival Cell membrane Cell model Cells Centrifugation Cerebrospinal Fluid Characteristics Chemiluminescence assay Chloroform Class Clinical Complex Computer software Condition Coomassie brilliant blue R Coumarins Culture Media Cultured Cells Custom Cysteine Cytolysis DNA DNA Modification Process Data Deoxyguanosine Deoxyribonuclease I Dependence Detection Detergents Development Dialysis procedure Diazomethane Diethyldithiocarbamate Digestion Dimethyl Sulfoxide Dinoprost Doxorubicin Doxycycline Dyes Edetic Acid Electrodes Electron Spin Resonance Spectroscopy Electronics Electrons Endothelial Cells Enhancers Enzymes Epithelial Epithelial Cells Equilibrium Equipment Esterified Fatty Acids Esters Ethanol Ethidium Etoposide Evaluation Exposure to FGF7 gene Fatty Acids Fiber Optics Figs - dietary Film Finland Fluorescence Fluorescence Microscopy Free Radicals Freezing Gases Gel Generations Genes Glass Glucose Glutathione HEPES HL-60 Cells Heating Hexanes High Pressure Liquid Chromatography High temperature of physical object Horseradish Peroxidase Hour Human Hydrochloride Salt Hydrogen Hydrogen Peroxide Hydrolysis Hydroxyl Radical Hyperoxia Ice Image Immunoblotting Incubated Individual Industrial Product Injection of therapeutic agent Injury Instruction Isopropanol Japan Kinetics Knockout Mice L-Ascorbate oxidase Label Laboratories Lasers Lecithin Left Length Life Light Link Lipid Peroxidation Lipids Lipoxygenase Liquid substance Longevity Luminol Lung Maleimides Manufacturer Name Measurement Measures Membrane Metabolic Metals Methanol Methodology Methods Microscope Microscopy Minor Modeling Modification Molecular Molecular Probes Molecular Sieve Chromatography Molecular Weight Monitor Monoclonal Antibodies Monophenol Monooxygenase Mus Myristates NADH NBL1 gene NOC-15 Natural regeneration Necrosis Nitrogen Nonesterified Fatty Acids Nuclease P1 Numbers Operant Conditioning Output Oxidants Oxidation-Reduction Oxidative Stress Oxides Oxygen Oxygenases PAPA NONOate PTEN gene Particle Size Performance Peroxidase Peroxidases Phase Phenols Phenolsulfonphthalein Phorbol Phorbols Phosphate Buffer Phosphatidylinositols Phospholipase A2 Phospholipids Phosphorus Physical Dialysis Plasma Potassium Phosphate Preparation Principal Investigator Procedures Process Production Prostaglandin-Endoperoxide Synthase Protein Overexpression Proteins Protocols documentation Pump Purpose Pyroxylin Quartz Rain Range Rate Rattus Reaction Reactive Oxygen Species Reader Reading Reagent Recycling Reporter Reporting Research Personnel Running Salts Sampling Sapphire Scanning Series Serum Serum Albumin Services Side Signal Transduction Site Sodium Acetate Sodium Azide Sodium Dodecyl Sulfate-PAGE Solid Solutions Sonication Source Speed Spin Trapping Staining method Stains Standards of Weights and Measures Stimulus Stream Stress Suction Sulfhydryl Compounds Superoxide Dismutase Superoxides Suspension substance Suspensions Sweden System TM 1 Techniques Teflon Temperature Testing Tetradecanoylphorbol Acetate Therapeutic Thick Time Tissue Extracts Tissue Sample Tissues Titanium Tocopherols Tokyo Training Transgenic Mice Triazoles Tube Tyrosine Ubiquinone Ultrasonics VP 16 Vertebrates Vitamin E Water Welts Width Wild Type Mouse Work adduct alpha benzopyrone alpha-(4-pyridyl-1-oxide)-N-tert-butylnitrone antitumor drug aqueous ascorbate attenuation autooxidation base biochemical model brain tissue catalase cell type cellular imaging charge coupled device camera coral cyclooxygenase 1 cyclooxygenase 2 data acquisition design detector diene digital dihydroethidium dinitrosyl iron complex ethyl acetate experience fetal fluorescence microscope gel electrophoresis human subject immunoreactivity in vivo inhibitor/antagonist inorganic phosphate instrument instrumentation irradiation isoprostaglandin F2alpha type-III keratinocyte growth factor lung injury macrophage microwave electromagnetic radiation mind control myristate neocuproine nitrone novel oxidation paraform parinaric acid perhydroxyl radical peroxidation phenoxy radical phosphoric diester hydrolase piperidine polyacrylamide gels prevent programs purge reconstitution repaired research study resorufin response sephadex G-75 sodium phosphate tetrahydrofuran time interval tissue culture trend two-photon ubiquinol ultrasphere-octadecyltrimethyloxysilane ultraviolet irradiation

中文摘要

翻译

该计划项目(PO1)的中心主题之一是急性肺损伤与增加产生的活性氧物种，这可能是氧化损伤的至少部分原因肺细胞。这表明，监测自由基、氧化应激和抗氧化状态的生物标志物对于了解急性肺损伤的发病机制和开发新的优化治疗方法是必不可少的战略。氧化应激的分析将在氧化应激生物标记物(Cobos)的核心上进行。该核心被设计成允许项目中的研究人员使用先进的技术来评估几个不同的相关参数。核心的具体目标是： 1.提供设计和实施实验的专业知识评估氧化应激和抗氧化剂生物标记物的适当技术。 2.准备、储存和优化分析组织、细胞和生物液样本，以检测自由基中间体，以及氧化应激的生物标记物生物分子的类别(蛋白质、DNA和脂类)。 3.对实验结果进行评价，并提出后续的实验方向。 4.提供关于使用和使用任何仪器和技术的培训该项目旨在评估氧化应激和抗氧化状态的生物标记物。总体而言，Cobos将是计划和执行氧化应激实验的核心 MD计划内的抗氧化剂。

英文摘要

One of the central topics of this Program Project (PO1) is that acute lung injury is associated with increased production of reactive oxygen species that may be responsible, at least in part, for oxidative injury to pulmonary cells. This suggests that monitoring free radicals, biomarkers of oxidative stress and antioxidant status is essential for understanding the mechanisms of acute lung injury and developing novel optimized therapeutic strategies. The assays of oxidative stress will be performed in the core on Biomarkers of Oxidative Stress (COBOS). The core has been designed to allow researchers in the projects to measure biomarkers of oxidative stress using sophisticated state-of-the art techniques to assess several of the different relevant parameters. Specific Aims of the core are: 1. Provide professional expertise in the design and implementation of experiments using adequate techniques for assessment of biomarkers of oxidative stress and antioxidants. 2. Prepare, store and optimally analyze samples of tissues, cells and biological fluids to detect free radical intermediates, as well as biomarkers of oxidative stress in major classes of biomolecules (proteins, DNA, and lipids). 3. Evaluate experimental results and propose subsequent experimental direction. 4. Provide training in the use of and access to any instrumentation and techniques used with the program project to assess biomarkers of oxidative stress and antioxidant status. Overall, COBOS will be central to the planning and execution of experiments on oxidative stress md antioxidants within the program.

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