Oxidative Lipidomics Core

目前，由于对ARDS的发病机制认识不足，目前尚无有效的药物治疗方法。P01的主要焦点是肺对一种新的肺毒素和调节因子的反应机制，这种新的肺毒素和调节因子是来自急性肺损伤期间受损宿主细胞的细菌或线粒体的阴离子磷脂(CL)。因此，阐明CL及其代谢物的不同分子物种的新的代谢和生化途径，以及它们与其他肺脂及其过氧化产物的相互作用，是P01所有四个项目的基础。氧化脂质组学核心(核心B)旨在使项目中的研究人员能够对CLS的所有主要分子物种、其他不同类别的脂质及其氧化产物进行详细的分析--鉴定、表征和成像。这将通过使用基于不同版本的质谱分析(MS)与液体色谱(LC)或高效薄层色谱(HPTLC)协议的尖端技术来实现。氧化脂质组学核心B的具体目标是：1.在设计和实施实验方面提供专业专业知识，使用适当的脂类鉴定、表征和定量技术，特别是CLS。制备和优化分析样品，以检测个别分子种类的脂类和氧化的脂类。2.为肺组织中不同类型的单个分子物种和氧化脂质提供质谱学成像的机会。3.对实验结果进行评价，并提出后续的实验方向。4.提供关于使用和获得P01项目中使用的任何仪器和技术的培训，以评估脂质组学/氧化脂质组学生物标记物。通过协助这些项目的分析工作，该核心将促进对CL作为急性肺损伤新的分子信号的机制的研究，从而有助于开发新的治疗方式。

Currently, there is no effective pharmacologic therapy against ARDS because of insufficient knowledge of the pathogenesis mechanisms. The overarching focus of the P01 is on mechanisms of pulmonary responses to a new pulmonary toxin and regulator, the anionic phosholipid, cardiolipin (CL) originating from bacteria or mitochondria of damaged host cells during acute lung injury. Thus elucidation of novel metabolic and biochemical pathways of different molecular species of CL, its metabolites and their interactions with other pulmonary lipids and their peroxidation products are fundamental to all four projects of the P01. The Oxidative Lipidomics Core (Core B) has been designed to allow researchers in the projects to perform detailed analysis - identification, characterization and imaging - of all major molecular species of Cls, other different classes of lipids as well as their oxidation products. This will be achieved by using sophisticated state-of-the art techniques based on different versions of mass-spectrometry (MS) combined with liquid¿ chromatography (LC) or high-performance thin-layer chromatography (HPTLC) protocols. Specific Aims of the Oxidative Lipidomics Core B are to: 1. Provide professional expertise in the design and implementation of experiments using adequate techniques for identification, characterization, and quantification of lipids, particularly Cls. Prepare and optimally analyze samples to detect individual molecular species of lipids and oxidized lipids. 2. Provide opportunities for mass-spectrometric imaging of different types of individual molecular species and oxidized lipids in lung tissues. 3. Evaluate experimental results and propose subsequent experimental direction. 4. Provide training in the use of and access to any instrumentations and techniques used within the P01 project to assess lipidomics/oxidative lipidomics biomarkers. By assisting the Projects in the analytical work, the Core will facilitate studies of the mechanisms through which CL functions as a new molecular signal in acute lung injury hence contributes to the development of new therapeutic modalities.